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Expression profile of the telomeric complex discriminates between benign and malignant pheochromocytoma.
J Clin Endocrinol Metab. 2003 Sep; 88(9):4280-6.JC

Abstract

Telomerase, a ribonucleoprotein complex that includes the telomerase RNA component, the telomerase-associated protein (TP1), the telomerase catalytic subunit (hTERT), and the heat shock protein 90 (HSP90), is closely related to the malignant potential of human tumors. In pheochromocytomas (PC) it is very difficult to predict malignant potential by conventional histology or immunohistochemical and molecular markers. To test whether the expression of telomerase subunits is reflected in the malignant transition of PCs, we determined their mRNA and/or protein expression in 28 benign and nine malignant PCs and compared the results with telomerase activity. RT-PCR analysis revealed that TP1 was ubiquitously expressed. The telomerase RNA component was found in all malignant (100%) and in 13 of 28 (46%) benign PCs. In contrast, hTERT was clearly associated with aggressive biological behavior. All of the malignant (100%), but only two of 28 benign (7%) PCs expressed hTERT. HSP90 was increased in malignant PCs, but was also expressed at a lower level in benign tumors. High telomerase activity was measurable in hTERT-positive tissues only. Our data indicate that hTERT, HSP90, and telomerase activity are up-regulated in malignant cells of the adrenal medulla. The common expression of hTERT and telomerase activity thus represents an additional prognostic marker that may identify more aggressive tumors.

Authors+Show Affiliations

Department of Pathology,Otto von Guericke University, D-39120 Magdeburg, Germany. carsten.boltze@medizin.uni-magdeburg.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12970299

Citation

Boltze, Carsten, et al. "Expression Profile of the Telomeric Complex Discriminates Between Benign and Malignant Pheochromocytoma." The Journal of Clinical Endocrinology and Metabolism, vol. 88, no. 9, 2003, pp. 4280-6.
Boltze C, Mundschenk J, Unger N, et al. Expression profile of the telomeric complex discriminates between benign and malignant pheochromocytoma. J Clin Endocrinol Metab. 2003;88(9):4280-6.
Boltze, C., Mundschenk, J., Unger, N., Schneider-Stock, R., Peters, B., Mawrin, C., Hoang-Vu, C., Roessner, A., & Lehnert, H. (2003). Expression profile of the telomeric complex discriminates between benign and malignant pheochromocytoma. The Journal of Clinical Endocrinology and Metabolism, 88(9), 4280-6.
Boltze C, et al. Expression Profile of the Telomeric Complex Discriminates Between Benign and Malignant Pheochromocytoma. J Clin Endocrinol Metab. 2003;88(9):4280-6. PubMed PMID: 12970299.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression profile of the telomeric complex discriminates between benign and malignant pheochromocytoma. AU - Boltze,Carsten, AU - Mundschenk,Jochen, AU - Unger,Nicole, AU - Schneider-Stock,Regine, AU - Peters,Brigitte, AU - Mawrin,Christian, AU - Hoang-Vu,Cuong, AU - Roessner,Albert, AU - Lehnert,Hendrik, PY - 2003/9/13/pubmed PY - 2003/10/11/medline PY - 2003/9/13/entrez SP - 4280 EP - 6 JF - The Journal of clinical endocrinology and metabolism JO - J Clin Endocrinol Metab VL - 88 IS - 9 N2 - Telomerase, a ribonucleoprotein complex that includes the telomerase RNA component, the telomerase-associated protein (TP1), the telomerase catalytic subunit (hTERT), and the heat shock protein 90 (HSP90), is closely related to the malignant potential of human tumors. In pheochromocytomas (PC) it is very difficult to predict malignant potential by conventional histology or immunohistochemical and molecular markers. To test whether the expression of telomerase subunits is reflected in the malignant transition of PCs, we determined their mRNA and/or protein expression in 28 benign and nine malignant PCs and compared the results with telomerase activity. RT-PCR analysis revealed that TP1 was ubiquitously expressed. The telomerase RNA component was found in all malignant (100%) and in 13 of 28 (46%) benign PCs. In contrast, hTERT was clearly associated with aggressive biological behavior. All of the malignant (100%), but only two of 28 benign (7%) PCs expressed hTERT. HSP90 was increased in malignant PCs, but was also expressed at a lower level in benign tumors. High telomerase activity was measurable in hTERT-positive tissues only. Our data indicate that hTERT, HSP90, and telomerase activity are up-regulated in malignant cells of the adrenal medulla. The common expression of hTERT and telomerase activity thus represents an additional prognostic marker that may identify more aggressive tumors. SN - 0021-972X UR - https://www.unboundmedicine.com/medline/citation/12970299/Expression_profile_of_the_telomeric_complex_discriminates_between_benign_and_malignant_pheochromocytoma_ DB - PRIME DP - Unbound Medicine ER -