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Rrp47p is an exosome-associated protein required for the 3' processing of stable RNAs.
Mol Cell Biol. 2003 Oct; 23(19):6982-92.MC

Abstract

Related exosome complexes of 3'-->5' exonucleases are present in the nucleus and the cytoplasm. Purification of exosome complexes from whole-cell lysates identified a Mg(2+)-labile factor present in substoichiometric amounts. This protein was identified as the nuclear protein Yhr081p, the homologue of human C1D, which we have designated Rrp47p (for rRNA processing). Immunoprecipitation of epitope-tagged Rrp47p confirmed its interaction with the exosome and revealed its association with Rrp6p, a 3'-->5' exonuclease specific to the nuclear exosome fraction. Northern analyses demonstrated that Rrp47p is required for the exosome-dependent processing of rRNA and small nucleolar RNA (snoRNA) precursors. Rrp47p also participates in the 3' processing of U4 and U5 small nuclear RNAs (snRNAs). The defects in the processing of stable RNAs seen in rrp47-Delta strains closely resemble those of strains lacking Rrp6p. In contrast, Rrp47p is not required for the Rrp6p-dependent degradation of 3'-extended nuclear pre-mRNAs or the cytoplasmic 3'-->5' mRNA decay pathway. We propose that Rrp47p functions as a substrate-specific nuclear cofactor for exosome activity in the processing of stable RNAs.

Authors+Show Affiliations

Wellcome Trust Centre for Cell Biology, Institute for Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, United Kingdom. pmitch@holyrood.ed.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12972615

Citation

Mitchell, Philip, et al. "Rrp47p Is an Exosome-associated Protein Required for the 3' Processing of Stable RNAs." Molecular and Cellular Biology, vol. 23, no. 19, 2003, pp. 6982-92.
Mitchell P, Petfalski E, Houalla R, et al. Rrp47p is an exosome-associated protein required for the 3' processing of stable RNAs. Mol Cell Biol. 2003;23(19):6982-92.
Mitchell, P., Petfalski, E., Houalla, R., Podtelejnikov, A., Mann, M., & Tollervey, D. (2003). Rrp47p is an exosome-associated protein required for the 3' processing of stable RNAs. Molecular and Cellular Biology, 23(19), 6982-92.
Mitchell P, et al. Rrp47p Is an Exosome-associated Protein Required for the 3' Processing of Stable RNAs. Mol Cell Biol. 2003;23(19):6982-92. PubMed PMID: 12972615.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rrp47p is an exosome-associated protein required for the 3' processing of stable RNAs. AU - Mitchell,Philip, AU - Petfalski,Elisabeth, AU - Houalla,Rym, AU - Podtelejnikov,Alexandre, AU - Mann,Matthias, AU - Tollervey,David, PY - 2003/9/16/pubmed PY - 2003/11/5/medline PY - 2003/9/16/entrez SP - 6982 EP - 92 JF - Molecular and cellular biology JO - Mol Cell Biol VL - 23 IS - 19 N2 - Related exosome complexes of 3'-->5' exonucleases are present in the nucleus and the cytoplasm. Purification of exosome complexes from whole-cell lysates identified a Mg(2+)-labile factor present in substoichiometric amounts. This protein was identified as the nuclear protein Yhr081p, the homologue of human C1D, which we have designated Rrp47p (for rRNA processing). Immunoprecipitation of epitope-tagged Rrp47p confirmed its interaction with the exosome and revealed its association with Rrp6p, a 3'-->5' exonuclease specific to the nuclear exosome fraction. Northern analyses demonstrated that Rrp47p is required for the exosome-dependent processing of rRNA and small nucleolar RNA (snoRNA) precursors. Rrp47p also participates in the 3' processing of U4 and U5 small nuclear RNAs (snRNAs). The defects in the processing of stable RNAs seen in rrp47-Delta strains closely resemble those of strains lacking Rrp6p. In contrast, Rrp47p is not required for the Rrp6p-dependent degradation of 3'-extended nuclear pre-mRNAs or the cytoplasmic 3'-->5' mRNA decay pathway. We propose that Rrp47p functions as a substrate-specific nuclear cofactor for exosome activity in the processing of stable RNAs. SN - 0270-7306 UR - https://www.unboundmedicine.com/medline/citation/12972615/Rrp47p_is_an_exosome_associated_protein_required_for_the_3'_processing_of_stable_RNAs_ DB - PRIME DP - Unbound Medicine ER -