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Interaction between XIAP and Smac/DIABLO in the mouse brain after transient focal cerebral ischemia.
J Cereb Blood Flow Metab. 2003 Sep; 23(9):1010-9.JC

Abstract

The X chromosome-linked inhibitor-of-apoptosis protein (XIAP) contributes to apoptosis regulation after a variety of cell death stimuli. XIAP inhibits the caspase reaction via binding to caspases, and is inhibited via binding to the second mitochondria-derived activator of caspase (Smac)/DIABLO to tightly control apoptotic cell death. However, the interaction among XIAP, Smac/DIABLO, and caspases after in vivo cerebral ischemia is not well known. To clarify this issue, the authors examined time-dependent expression and interaction among XIAP, Smac/DIABLO, and activated caspase-9 by immunohistochemistry, Western blot analysis, and immunoprecipitation using an in vivo transient focal cerebral ischemia model. To examine the relationship of the XIAP pathway to the caspase cascade, a pan-caspase inhibitor was administered. XIAP increased concurrently with the release of Smac/DIABLO and the appearance of activated caspase-9 during the early period after reperfusion injury. The bindings of XIAP to Smac/DIABLO and to caspase-9 and the binding of Smac/DIABLO to caspase-9 reached a peak simultaneously after transient focal cerebral ischemia. Neither XIAP nor Smac/DIABLO expression was affected by caspase inhibition. These results suggest that the XIAP pathway was activated upstream of the caspase cascade and that interaction among XIAP, Smac/DIABLO, and caspase-9 plays an important role in the regulation of apoptotic neuronal cell death after transient focal cerebral ischemia.

Authors+Show Affiliations

Department of Neurosurgery, Stanford University School of Medicine, Stanford, CA 94305, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12973017

Citation

Saito, Atsushi, et al. "Interaction Between XIAP and Smac/DIABLO in the Mouse Brain After Transient Focal Cerebral Ischemia." Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism, vol. 23, no. 9, 2003, pp. 1010-9.
Saito A, Hayashi T, Okuno S, et al. Interaction between XIAP and Smac/DIABLO in the mouse brain after transient focal cerebral ischemia. J Cereb Blood Flow Metab. 2003;23(9):1010-9.
Saito, A., Hayashi, T., Okuno, S., Ferrand-Drake, M., & Chan, P. H. (2003). Interaction between XIAP and Smac/DIABLO in the mouse brain after transient focal cerebral ischemia. Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism, 23(9), 1010-9.
Saito A, et al. Interaction Between XIAP and Smac/DIABLO in the Mouse Brain After Transient Focal Cerebral Ischemia. J Cereb Blood Flow Metab. 2003;23(9):1010-9. PubMed PMID: 12973017.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interaction between XIAP and Smac/DIABLO in the mouse brain after transient focal cerebral ischemia. AU - Saito,Atsushi, AU - Hayashi,Takeshi, AU - Okuno,Shuzo, AU - Ferrand-Drake,Michel, AU - Chan,Pak H, PY - 2003/9/16/pubmed PY - 2003/10/17/medline PY - 2003/9/16/entrez SP - 1010 EP - 9 JF - Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism JO - J Cereb Blood Flow Metab VL - 23 IS - 9 N2 - The X chromosome-linked inhibitor-of-apoptosis protein (XIAP) contributes to apoptosis regulation after a variety of cell death stimuli. XIAP inhibits the caspase reaction via binding to caspases, and is inhibited via binding to the second mitochondria-derived activator of caspase (Smac)/DIABLO to tightly control apoptotic cell death. However, the interaction among XIAP, Smac/DIABLO, and caspases after in vivo cerebral ischemia is not well known. To clarify this issue, the authors examined time-dependent expression and interaction among XIAP, Smac/DIABLO, and activated caspase-9 by immunohistochemistry, Western blot analysis, and immunoprecipitation using an in vivo transient focal cerebral ischemia model. To examine the relationship of the XIAP pathway to the caspase cascade, a pan-caspase inhibitor was administered. XIAP increased concurrently with the release of Smac/DIABLO and the appearance of activated caspase-9 during the early period after reperfusion injury. The bindings of XIAP to Smac/DIABLO and to caspase-9 and the binding of Smac/DIABLO to caspase-9 reached a peak simultaneously after transient focal cerebral ischemia. Neither XIAP nor Smac/DIABLO expression was affected by caspase inhibition. These results suggest that the XIAP pathway was activated upstream of the caspase cascade and that interaction among XIAP, Smac/DIABLO, and caspase-9 plays an important role in the regulation of apoptotic neuronal cell death after transient focal cerebral ischemia. SN - 0271-678X UR - https://www.unboundmedicine.com/medline/citation/12973017/Interaction_between_XIAP_and_Smac/DIABLO_in_the_mouse_brain_after_transient_focal_cerebral_ischemia_ L2 - https://journals.sagepub.com/doi/10.1097/01.WCB.0000080702.47016.FF?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -