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Molecular cloning and physical mapping of the otsBA genes, which encode the osmoregulatory trehalose pathway of Escherichia coli: evidence that transcription is activated by katF (AppR)
J Bacteriol 1992; 174(3):889-98JB

Abstract

It has been shown previously that the otsA and otsB mutations block osmoregulatory trehalose synthesis in Escherichia coli. We report that the transcription of these osmoregulated ots genes is dependent on KatF (AppR), a putative sigma factor for certain stationary phase- and starvation-induced genes. The transcription of the osmoregulated bet and proU genes was not katF dependent. Our genetic analysis showed that katF carries an amber mutation in E. coli K-12 and many of its derivatives but that katF has reverted to an active form in the much-used strain MC4100. This amber mutation in katF leads to strain variations in trehalose synthesis and other katF-dependent functions of E. coli. We have performed a molecular cloning of the otsBA genes, and we present evidence that they constitute an operon encoding trehalose-6-phosphate phosphatase and trehalose-6-phosphate synthase. A cloning and restriction site analysis, performed by comparing the cloned fragments with the known physical map of the E. coli chromosome, revealed that the otsBA genes are situated on a 2.9-kb HindIII fragment located 8 to 11 kb clockwise of tar (41.6 min).

Authors+Show Affiliations

Norwegian College of Fishery Science, University of Tromsø, Norway.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1310094

Citation

Kaasen, I, et al. "Molecular Cloning and Physical Mapping of the otsBA Genes, Which Encode the Osmoregulatory Trehalose Pathway of Escherichia Coli: Evidence That Transcription Is Activated By katF (AppR)." Journal of Bacteriology, vol. 174, no. 3, 1992, pp. 889-98.
Kaasen I, Falkenberg P, Styrvold OB, et al. Molecular cloning and physical mapping of the otsBA genes, which encode the osmoregulatory trehalose pathway of Escherichia coli: evidence that transcription is activated by katF (AppR). J Bacteriol. 1992;174(3):889-98.
Kaasen, I., Falkenberg, P., Styrvold, O. B., & Strøm, A. R. (1992). Molecular cloning and physical mapping of the otsBA genes, which encode the osmoregulatory trehalose pathway of Escherichia coli: evidence that transcription is activated by katF (AppR). Journal of Bacteriology, 174(3), pp. 889-98.
Kaasen I, et al. Molecular Cloning and Physical Mapping of the otsBA Genes, Which Encode the Osmoregulatory Trehalose Pathway of Escherichia Coli: Evidence That Transcription Is Activated By katF (AppR). J Bacteriol. 1992;174(3):889-98. PubMed PMID: 1310094.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular cloning and physical mapping of the otsBA genes, which encode the osmoregulatory trehalose pathway of Escherichia coli: evidence that transcription is activated by katF (AppR) AU - Kaasen,I, AU - Falkenberg,P, AU - Styrvold,O B, AU - Strøm,A R, PY - 1992/2/1/pubmed PY - 1992/2/1/medline PY - 1992/2/1/entrez SP - 889 EP - 98 JF - Journal of bacteriology JO - J. Bacteriol. VL - 174 IS - 3 N2 - It has been shown previously that the otsA and otsB mutations block osmoregulatory trehalose synthesis in Escherichia coli. We report that the transcription of these osmoregulated ots genes is dependent on KatF (AppR), a putative sigma factor for certain stationary phase- and starvation-induced genes. The transcription of the osmoregulated bet and proU genes was not katF dependent. Our genetic analysis showed that katF carries an amber mutation in E. coli K-12 and many of its derivatives but that katF has reverted to an active form in the much-used strain MC4100. This amber mutation in katF leads to strain variations in trehalose synthesis and other katF-dependent functions of E. coli. We have performed a molecular cloning of the otsBA genes, and we present evidence that they constitute an operon encoding trehalose-6-phosphate phosphatase and trehalose-6-phosphate synthase. A cloning and restriction site analysis, performed by comparing the cloned fragments with the known physical map of the E. coli chromosome, revealed that the otsBA genes are situated on a 2.9-kb HindIII fragment located 8 to 11 kb clockwise of tar (41.6 min). SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/1310094/Molecular_cloning_and_physical_mapping_of_the_otsBA_genes_which_encode_the_osmoregulatory_trehalose_pathway_of_Escherichia_coli:_evidence_that_transcription_is_activated_by_katF__AppR_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=1310094 DB - PRIME DP - Unbound Medicine ER -