Tags

Type your tag names separated by a space and hit enter

Histamine and calcium are independently regulated intracellular mediators of lymphocyte mitogenesis.
Biochem Biophys Res Commun. 1992 Jan 31; 182(2):786-93.BB

Abstract

In addition to cytosolic calcium ([Ca2+]i), intracellular histamine has been implicated as a mediator of mitogenesis in normal mouse spleen cells stimulated by the plant lectin, concanavalin (Con) A. We have linked the growth-promoting action of this amine with its binding to distinct intracellular sites, designated HIC, in microsomes and nuclei and shown that the proliferative response of lymphocytes can be blocked by antagonizing the binding of histamine to HIC by N,N-diethyl-2-[4-(phenylmethyl)phenoxy]ethanamine.HCl (DPPE), or by depleting intracellular histamine levels by the specific irreversible inhibitor of histidine decarboxylase, alpha-FMH. We now demonstrate that, at a concentration which completely inhibits both 3H-histamine binding to HIC and 3H-thymidine incorporation into DNA, DPPE fails to block the acute (30 seconds) rise in [Ca2+]i in spleen cells exposed to Con A. Conversely, the calcium channel antagonist, verapamil, suppresses the Con A-induced rise in [Ca2+]i at a concentration which correlates with its inhibition of thymidine incorporation into DNA, but does not prevent histamine synthesis or bind to HIC. Thus, in Con A-stimulated lymphocytes, intracellular histamine and calcium appear to be independently regulated, but essential, mediators of the proliferative response.

Authors+Show Affiliations

Brandes LJ
Department of Medicine, Faculty of Medicine, University of Manitoba.
LaBella FS
No affiliation info available

MeSH

AnimalsCalciumCalcium ChannelsCell MembraneCells, CulturedCerebral CortexCimetidineConcanavalin ADNA ReplicationHistamineHistamine AntagonistsKineticsLymphocytesMiceMice, Inbred BALB CPhenyl EthersPyrilamineRatsReceptors, Histamine H1Receptors, NicotinicSpleenVerapamil

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1310395

Citation

Brandes, L J., and F S. LaBella. "Histamine and Calcium Are Independently Regulated Intracellular Mediators of Lymphocyte Mitogenesis." Biochemical and Biophysical Research Communications, vol. 182, no. 2, 1992, pp. 786-93.
Brandes LJ, LaBella FS. Histamine and calcium are independently regulated intracellular mediators of lymphocyte mitogenesis. Biochem Biophys Res Commun. 1992;182(2):786-93.
Brandes, L. J., & LaBella, F. S. (1992). Histamine and calcium are independently regulated intracellular mediators of lymphocyte mitogenesis. Biochemical and Biophysical Research Communications, 182(2), 786-93.
Brandes LJ, LaBella FS. Histamine and Calcium Are Independently Regulated Intracellular Mediators of Lymphocyte Mitogenesis. Biochem Biophys Res Commun. 1992 Jan 31;182(2):786-93. PubMed PMID: 1310395.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Histamine and calcium are independently regulated intracellular mediators of lymphocyte mitogenesis. AU - Brandes,L J, AU - LaBella,F S, PY - 1992/1/31/pubmed PY - 1992/1/31/medline PY - 1992/1/31/entrez SP - 786 EP - 93 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 182 IS - 2 N2 - In addition to cytosolic calcium ([Ca2+]i), intracellular histamine has been implicated as a mediator of mitogenesis in normal mouse spleen cells stimulated by the plant lectin, concanavalin (Con) A. We have linked the growth-promoting action of this amine with its binding to distinct intracellular sites, designated HIC, in microsomes and nuclei and shown that the proliferative response of lymphocytes can be blocked by antagonizing the binding of histamine to HIC by N,N-diethyl-2-[4-(phenylmethyl)phenoxy]ethanamine.HCl (DPPE), or by depleting intracellular histamine levels by the specific irreversible inhibitor of histidine decarboxylase, alpha-FMH. We now demonstrate that, at a concentration which completely inhibits both 3H-histamine binding to HIC and 3H-thymidine incorporation into DNA, DPPE fails to block the acute (30 seconds) rise in [Ca2+]i in spleen cells exposed to Con A. Conversely, the calcium channel antagonist, verapamil, suppresses the Con A-induced rise in [Ca2+]i at a concentration which correlates with its inhibition of thymidine incorporation into DNA, but does not prevent histamine synthesis or bind to HIC. Thus, in Con A-stimulated lymphocytes, intracellular histamine and calcium appear to be independently regulated, but essential, mediators of the proliferative response. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/1310395/Histamine_and_calcium_are_independently_regulated_intracellular_mediators_of_lymphocyte_mitogenesis_ DB - PRIME DP - Unbound Medicine ER -