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Cloning and identification of the Escherichia coli murB DNA sequence, which encodes UDP-N-acetylenolpyruvoylglucosamine reductase.
J Bacteriol. 1992 Mar; 174(5):1690-3.JB

Abstract

The murB gene, which complemented the UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.1.1.158) mutation in Escherichia coli ST5, was cloned from an E. coli chromosomal library. murB was subcloned on a 2.8-kb PvuII fragment into pUC19 and sequenced. A 1,029-bp open reading frame encoded a 342-amino-acid polypeptide of 37,859 Da. A DNA sequence homology search revealed that murB had almost 100% homology with a previously reported unidentified open reading frame, ORFII, at 89.9 min. Physical and genetic mapping results were consistent with this map position, and minicell analyses of murB subclones showed a plasmid-encoded protein of approximately 37,000 Da, which closely matched the calculated size of the murB protein.

Authors+Show Affiliations

Department of Microbiology, Bristol-Myers Squibb Company, Wallingford, Connecticut 06492-7660.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1311302

Citation

Pucci, M J., et al. "Cloning and Identification of the Escherichia Coli murB DNA Sequence, Which Encodes UDP-N-acetylenolpyruvoylglucosamine Reductase." Journal of Bacteriology, vol. 174, no. 5, 1992, pp. 1690-3.
Pucci MJ, Discotto LF, Dougherty TJ. Cloning and identification of the Escherichia coli murB DNA sequence, which encodes UDP-N-acetylenolpyruvoylglucosamine reductase. J Bacteriol. 1992;174(5):1690-3.
Pucci, M. J., Discotto, L. F., & Dougherty, T. J. (1992). Cloning and identification of the Escherichia coli murB DNA sequence, which encodes UDP-N-acetylenolpyruvoylglucosamine reductase. Journal of Bacteriology, 174(5), 1690-3.
Pucci MJ, Discotto LF, Dougherty TJ. Cloning and Identification of the Escherichia Coli murB DNA Sequence, Which Encodes UDP-N-acetylenolpyruvoylglucosamine Reductase. J Bacteriol. 1992;174(5):1690-3. PubMed PMID: 1311302.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning and identification of the Escherichia coli murB DNA sequence, which encodes UDP-N-acetylenolpyruvoylglucosamine reductase. AU - Pucci,M J, AU - Discotto,L F, AU - Dougherty,T J, PY - 1992/3/1/pubmed PY - 1992/3/1/medline PY - 1992/3/1/entrez SP - 1690 EP - 3 JF - Journal of bacteriology JO - J Bacteriol VL - 174 IS - 5 N2 - The murB gene, which complemented the UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.1.1.158) mutation in Escherichia coli ST5, was cloned from an E. coli chromosomal library. murB was subcloned on a 2.8-kb PvuII fragment into pUC19 and sequenced. A 1,029-bp open reading frame encoded a 342-amino-acid polypeptide of 37,859 Da. A DNA sequence homology search revealed that murB had almost 100% homology with a previously reported unidentified open reading frame, ORFII, at 89.9 min. Physical and genetic mapping results were consistent with this map position, and minicell analyses of murB subclones showed a plasmid-encoded protein of approximately 37,000 Da, which closely matched the calculated size of the murB protein. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/1311302/Cloning_and_identification_of_the_Escherichia_coli_murB_DNA_sequence_which_encodes_UDP_N_acetylenolpyruvoylglucosamine_reductase_ L2 - https://journals.asm.org/doi/10.1128/jb.174.5.1690-1693.1992?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -