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Protective effect of supplementation of fish oil with high n-3 polyunsaturated fatty acids against oxidative stress-induced DNA damage of rat liver in vivo.
J Agric Food Chem. 2003 Sep 24; 51(20):6073-9.JA

Abstract

The present study was undertaken to know the effect of supplementation of fish oil with high n-3 polyunsaturated fatty acids (PUFA) on oxidative stress-induced DNA damage of rat liver in vivo. Male Wistar rats were fed a diet containing fish oil or safflower oil with high n-6 PUFA at 50 g/kg of diet and an equal amount of vitamin E at 59 mg/kg of diet for 6 weeks. Livers of rats fed fish oil were rich in n-3 PUFA, whereas those of rats fed safflower oil were rich in n-6 PUFA. Ferric nitrilotriacetate was intraperitoneally injected to induce oxidative stress. The degree of lipid peroxidation of the liver was assessed by the levels of phospholipid hydroperoxides and thiobarbituric acid-reactive substances (TBARS), and the degree of oxidative DNA damage was assessed by comet type characterization in alkaline single-cell gel electrophoresis and 8-hydroxy-2'-deoxyguanosine levels. The levels of TBARS of the livers of the fish oil diet group increased to a greater extent than those of the safflower oil diet group, whereas the levels of the hydroperoxides of the livers of both diet groups increased to a similar extent. The vitamin E level of livers of the fish oil diet group was remarkably decreased. The degree of DNA damage of both diet groups was increased, but the increased level of the fish oil diet group was remarkably lower than that of the safflower oil diet group. The above results indicate that fish oil supplementation does not enhance but appears to protect against oxidative stress-induced DNA damage and suggest that lipid peroxidation does not enhance but lowers the DNA damage.

Authors+Show Affiliations

School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan. kikugawa@ps.toyaku.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

13129319

Citation

Kikugawa, Kiyomi, et al. "Protective Effect of Supplementation of Fish Oil With High N-3 Polyunsaturated Fatty Acids Against Oxidative Stress-induced DNA Damage of Rat Liver in Vivo." Journal of Agricultural and Food Chemistry, vol. 51, no. 20, 2003, pp. 6073-9.
Kikugawa K, Yasuhara Y, Ando K, et al. Protective effect of supplementation of fish oil with high n-3 polyunsaturated fatty acids against oxidative stress-induced DNA damage of rat liver in vivo. J Agric Food Chem. 2003;51(20):6073-9.
Kikugawa, K., Yasuhara, Y., Ando, K., Koyama, K., Hiramoto, K., & Suzuki, M. (2003). Protective effect of supplementation of fish oil with high n-3 polyunsaturated fatty acids against oxidative stress-induced DNA damage of rat liver in vivo. Journal of Agricultural and Food Chemistry, 51(20), 6073-9.
Kikugawa K, et al. Protective Effect of Supplementation of Fish Oil With High N-3 Polyunsaturated Fatty Acids Against Oxidative Stress-induced DNA Damage of Rat Liver in Vivo. J Agric Food Chem. 2003 Sep 24;51(20):6073-9. PubMed PMID: 13129319.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of supplementation of fish oil with high n-3 polyunsaturated fatty acids against oxidative stress-induced DNA damage of rat liver in vivo. AU - Kikugawa,Kiyomi, AU - Yasuhara,Yoshinobu, AU - Ando,Ken, AU - Koyama,Keiko, AU - Hiramoto,Kazuyuki, AU - Suzuki,Masao, PY - 2003/9/18/pubmed PY - 2003/10/28/medline PY - 2003/9/18/entrez SP - 6073 EP - 9 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 51 IS - 20 N2 - The present study was undertaken to know the effect of supplementation of fish oil with high n-3 polyunsaturated fatty acids (PUFA) on oxidative stress-induced DNA damage of rat liver in vivo. Male Wistar rats were fed a diet containing fish oil or safflower oil with high n-6 PUFA at 50 g/kg of diet and an equal amount of vitamin E at 59 mg/kg of diet for 6 weeks. Livers of rats fed fish oil were rich in n-3 PUFA, whereas those of rats fed safflower oil were rich in n-6 PUFA. Ferric nitrilotriacetate was intraperitoneally injected to induce oxidative stress. The degree of lipid peroxidation of the liver was assessed by the levels of phospholipid hydroperoxides and thiobarbituric acid-reactive substances (TBARS), and the degree of oxidative DNA damage was assessed by comet type characterization in alkaline single-cell gel electrophoresis and 8-hydroxy-2'-deoxyguanosine levels. The levels of TBARS of the livers of the fish oil diet group increased to a greater extent than those of the safflower oil diet group, whereas the levels of the hydroperoxides of the livers of both diet groups increased to a similar extent. The vitamin E level of livers of the fish oil diet group was remarkably decreased. The degree of DNA damage of both diet groups was increased, but the increased level of the fish oil diet group was remarkably lower than that of the safflower oil diet group. The above results indicate that fish oil supplementation does not enhance but appears to protect against oxidative stress-induced DNA damage and suggest that lipid peroxidation does not enhance but lowers the DNA damage. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/13129319/Protective_effect_of_supplementation_of_fish_oil_with_high_n_3_polyunsaturated_fatty_acids_against_oxidative_stress_induced_DNA_damage_of_rat_liver_in_vivo_ L2 - https://dx.doi.org/10.1021/jf030141v DB - PRIME DP - Unbound Medicine ER -