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Expression of CD137 and its ligand in human neurons, astrocytes, and microglia: modulation by FGF-2.
J Neurosci Res. 2003 Oct 01; 74(1):67-73.JN

Abstract

CD137 (ILA, 4-1BB), a member of the tumor necrosis factor receptor family, and its ligand CD137-L were assayed by RT-PCR and immunocytochemistry in cultured human brain cells. Results demonstrated that both neurons and astrocytes expressed specific RNA for CD137 and its protein, which was found both on the plasma membrane and in the cytoplasm. Surprisingly, microglia, which also expressed CD137 mRNA, showed negative immunostaining. CD137-L-specific RNA was detected only in astrocytes and neurons. When brain cells were treated with fibroblast growth factor-2 (FGF-2), upregulation of CD137 but not of its ligand was observed in neurons and astrocytes. Protein localization was also affected. In microglia, an inhibition of RNA expression was induced by treatment, whereas CD137-L remained negative. Our data are the first demonstration that human brain cells express a protein found thus far in activated immunocompetent cells and epithelia. Moreover, they suggest not only that CD137 and CD137-L might play a role in interaction among human brain cells, but also that FGF-2 might have an immunoregulatory function in brain, modulating interaction of the central nervous system with peripheral immunocompetent cells.

Authors+Show Affiliations

Department of Cytomorphology, School of Medicine, University of Cagliari, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

13130507

Citation

Reali, Camilla, et al. "Expression of CD137 and Its Ligand in Human Neurons, Astrocytes, and Microglia: Modulation By FGF-2." Journal of Neuroscience Research, vol. 74, no. 1, 2003, pp. 67-73.
Reali C, Curto M, Sogos V, et al. Expression of CD137 and its ligand in human neurons, astrocytes, and microglia: modulation by FGF-2. J Neurosci Res. 2003;74(1):67-73.
Reali, C., Curto, M., Sogos, V., Scintu, F., Pauly, S., Schwarz, H., & Gremo, F. (2003). Expression of CD137 and its ligand in human neurons, astrocytes, and microglia: modulation by FGF-2. Journal of Neuroscience Research, 74(1), 67-73.
Reali C, et al. Expression of CD137 and Its Ligand in Human Neurons, Astrocytes, and Microglia: Modulation By FGF-2. J Neurosci Res. 2003 Oct 1;74(1):67-73. PubMed PMID: 13130507.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of CD137 and its ligand in human neurons, astrocytes, and microglia: modulation by FGF-2. AU - Reali,Camilla, AU - Curto,Monica, AU - Sogos,Valeria, AU - Scintu,Franca, AU - Pauly,Susanne, AU - Schwarz,Herbert, AU - Gremo,Fulvia, PY - 2003/9/18/pubmed PY - 2003/11/11/medline PY - 2003/9/18/entrez SP - 67 EP - 73 JF - Journal of neuroscience research JO - J Neurosci Res VL - 74 IS - 1 N2 - CD137 (ILA, 4-1BB), a member of the tumor necrosis factor receptor family, and its ligand CD137-L were assayed by RT-PCR and immunocytochemistry in cultured human brain cells. Results demonstrated that both neurons and astrocytes expressed specific RNA for CD137 and its protein, which was found both on the plasma membrane and in the cytoplasm. Surprisingly, microglia, which also expressed CD137 mRNA, showed negative immunostaining. CD137-L-specific RNA was detected only in astrocytes and neurons. When brain cells were treated with fibroblast growth factor-2 (FGF-2), upregulation of CD137 but not of its ligand was observed in neurons and astrocytes. Protein localization was also affected. In microglia, an inhibition of RNA expression was induced by treatment, whereas CD137-L remained negative. Our data are the first demonstration that human brain cells express a protein found thus far in activated immunocompetent cells and epithelia. Moreover, they suggest not only that CD137 and CD137-L might play a role in interaction among human brain cells, but also that FGF-2 might have an immunoregulatory function in brain, modulating interaction of the central nervous system with peripheral immunocompetent cells. SN - 0360-4012 UR - https://www.unboundmedicine.com/medline/citation/13130507/Expression_of_CD137_and_its_ligand_in_human_neurons_astrocytes_and_microglia:_modulation_by_FGF_2_ L2 - https://doi.org/10.1002/jnr.10727 DB - PRIME DP - Unbound Medicine ER -