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[Second generation hepatitis-C virus test and polymerase chain reaction in anti-C 100 negative patients with chronic non-A, non-B hepatitis].
Schweiz Med Wochenschr 1992; 122(11):363-8SM

Abstract

The aim of our study was to evaluate whether a negative HCV test of the first generation (HCV-ELISA 1) using the antigen C100-3 excludes chronic HCV infection, or whether patients exist who are negative for antibodies to C100-3 in spite of chronic hepatitis C. 27 patients with histologically proven chronic non-A, non-B hepatitis, all of whom were HCV-ELISA 1 negative, were tested by the HCV test systems of the second generation (Ortho-HCV-ELISA 2 and Chiron-HCV-RIBA 2) based on the distinct HCV antigens 5-1-1, C100-3, C33c and C22-3. To determine the presence of viremia, serum samples were also tested for HCV-RNA with "nested" PCR. 10 of 27 patients proved to be persistently negative when tested with the second generation assays. One patient showed low grade reactivity by HCV-ELISA 2, but non-reactivity by HCV-RIBA 2. In none of these 11 patients was HCV-RNA detected. 16 (60%) of 27 patients negative with HCV-ELISA 1 were positive with HCV-ELISA 2. HCV-RIBA 2 detected antibodies to the structural core antigen C22-3 in all of these 16 patients and antibodies to the non-structural antigen C33c in 14 of them, while antibodies to 5-1-1 or C100-3 were not found in any of these cases. 10 (63%) of the 16 HCV-ELISA 1 negative, but HCV-ELISA 2 and HCV-RIBA 2 positive patients were positive for HCV-RNA by "nested" PCR.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Medizinische Klinik, Stadtspital Waid, Zürich.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
English Abstract
Journal Article

Language

ger

PubMed ID

1313601

Citation

Zala, G, et al. "[Second Generation hepatitis-C Virus Test and Polymerase Chain Reaction in anti-C 100 Negative Patients With Chronic non-A, non-B Hepatitis]." Schweizerische Medizinische Wochenschrift, vol. 122, no. 11, 1992, pp. 363-8.
Zala G, Havelka J, Wicki AN, et al. [Second generation hepatitis-C virus test and polymerase chain reaction in anti-C 100 negative patients with chronic non-A, non-B hepatitis]. Schweiz Med Wochenschr. 1992;122(11):363-8.
Zala, G., Havelka, J., Wicki, A. N., Joller-Jemelka, H. I., Meier, B., Schmid, M., & Bühler, H. (1992). [Second generation hepatitis-C virus test and polymerase chain reaction in anti-C 100 negative patients with chronic non-A, non-B hepatitis]. Schweizerische Medizinische Wochenschrift, 122(11), pp. 363-8.
Zala G, et al. [Second Generation hepatitis-C Virus Test and Polymerase Chain Reaction in anti-C 100 Negative Patients With Chronic non-A, non-B Hepatitis]. Schweiz Med Wochenschr. 1992 Mar 14;122(11):363-8. PubMed PMID: 1313601.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Second generation hepatitis-C virus test and polymerase chain reaction in anti-C 100 negative patients with chronic non-A, non-B hepatitis]. AU - Zala,G, AU - Havelka,J, AU - Wicki,A N, AU - Joller-Jemelka,H I, AU - Meier,B, AU - Schmid,M, AU - Bühler,H, PY - 1992/3/14/pubmed PY - 1992/3/14/medline PY - 1992/3/14/entrez SP - 363 EP - 8 JF - Schweizerische medizinische Wochenschrift JO - Schweiz Med Wochenschr VL - 122 IS - 11 N2 - The aim of our study was to evaluate whether a negative HCV test of the first generation (HCV-ELISA 1) using the antigen C100-3 excludes chronic HCV infection, or whether patients exist who are negative for antibodies to C100-3 in spite of chronic hepatitis C. 27 patients with histologically proven chronic non-A, non-B hepatitis, all of whom were HCV-ELISA 1 negative, were tested by the HCV test systems of the second generation (Ortho-HCV-ELISA 2 and Chiron-HCV-RIBA 2) based on the distinct HCV antigens 5-1-1, C100-3, C33c and C22-3. To determine the presence of viremia, serum samples were also tested for HCV-RNA with "nested" PCR. 10 of 27 patients proved to be persistently negative when tested with the second generation assays. One patient showed low grade reactivity by HCV-ELISA 2, but non-reactivity by HCV-RIBA 2. In none of these 11 patients was HCV-RNA detected. 16 (60%) of 27 patients negative with HCV-ELISA 1 were positive with HCV-ELISA 2. HCV-RIBA 2 detected antibodies to the structural core antigen C22-3 in all of these 16 patients and antibodies to the non-structural antigen C33c in 14 of them, while antibodies to 5-1-1 or C100-3 were not found in any of these cases. 10 (63%) of the 16 HCV-ELISA 1 negative, but HCV-ELISA 2 and HCV-RIBA 2 positive patients were positive for HCV-RNA by "nested" PCR.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0036-7672 UR - https://www.unboundmedicine.com/medline/citation/1313601/[Second_generation_hepatitis_C_virus_test_and_polymerase_chain_reaction_in_anti_C_100_negative_patients_with_chronic_non_A_non_B_hepatitis]_ L2 - http://www.diseaseinfosearch.org/result/3332 DB - PRIME DP - Unbound Medicine ER -