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Hypoxia injures endothelial cells by increasing endogenous xanthine oxidase activity.
Proc Natl Acad Sci U S A. 1992 Apr 15; 89(8):3362-6.PN

Abstract

Exposure to decreasing oxygen tensions progressively increased xanthine dehydrogenase (XD) and xanthine oxidase (XO) activities over 48 hr in cultured pulmonary artery endothelial cells (EC) without altering XD/XO ratios. Increases in XD and XO activity in EC induced by hypoxia were associated upon reoxygenation with increased (P less than 0.05) extracellular superoxide anion (O2-.) levels that were inhibited by treatment with XO inhibitors (tungsten, allopurinol) or an anion-channel blocker (4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid). EC monolayers subjected to hypoxia/reoxygenation also leaked more preloaded 51Cr, were more adherent to neutrophils, and permitted greater albumin transit than control monolayers. Treatment with tungsten, allopurinol, and/or superoxide dismutase decreased (P less than 0.05) 51Cr release, neutrophil adherence, and albumin transit in EC monolayers exposed to hypoxia/reoxygenation. We conclude that prolonged hypoxia increases both XO and XD activity in EC and may predispose the endothelium to oxidative and inflammatory damage.

Authors+Show Affiliations

Department of Medicine, University of Colorado Health Sciences Center, Denver.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1314387

Citation

Terada, L S., et al. "Hypoxia Injures Endothelial Cells By Increasing Endogenous Xanthine Oxidase Activity." Proceedings of the National Academy of Sciences of the United States of America, vol. 89, no. 8, 1992, pp. 3362-6.
Terada LS, Guidot DM, Leff JA, et al. Hypoxia injures endothelial cells by increasing endogenous xanthine oxidase activity. Proc Natl Acad Sci U S A. 1992;89(8):3362-6.
Terada, L. S., Guidot, D. M., Leff, J. A., Willingham, I. R., Hanley, M. E., Piermattei, D., & Repine, J. E. (1992). Hypoxia injures endothelial cells by increasing endogenous xanthine oxidase activity. Proceedings of the National Academy of Sciences of the United States of America, 89(8), 3362-6.
Terada LS, et al. Hypoxia Injures Endothelial Cells By Increasing Endogenous Xanthine Oxidase Activity. Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3362-6. PubMed PMID: 1314387.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hypoxia injures endothelial cells by increasing endogenous xanthine oxidase activity. AU - Terada,L S, AU - Guidot,D M, AU - Leff,J A, AU - Willingham,I R, AU - Hanley,M E, AU - Piermattei,D, AU - Repine,J E, PY - 1992/4/15/pubmed PY - 1992/4/15/medline PY - 1992/4/15/entrez SP - 3362 EP - 6 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 89 IS - 8 N2 - Exposure to decreasing oxygen tensions progressively increased xanthine dehydrogenase (XD) and xanthine oxidase (XO) activities over 48 hr in cultured pulmonary artery endothelial cells (EC) without altering XD/XO ratios. Increases in XD and XO activity in EC induced by hypoxia were associated upon reoxygenation with increased (P less than 0.05) extracellular superoxide anion (O2-.) levels that were inhibited by treatment with XO inhibitors (tungsten, allopurinol) or an anion-channel blocker (4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid). EC monolayers subjected to hypoxia/reoxygenation also leaked more preloaded 51Cr, were more adherent to neutrophils, and permitted greater albumin transit than control monolayers. Treatment with tungsten, allopurinol, and/or superoxide dismutase decreased (P less than 0.05) 51Cr release, neutrophil adherence, and albumin transit in EC monolayers exposed to hypoxia/reoxygenation. We conclude that prolonged hypoxia increases both XO and XD activity in EC and may predispose the endothelium to oxidative and inflammatory damage. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/1314387/Hypoxia_injures_endothelial_cells_by_increasing_endogenous_xanthine_oxidase_activity_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=1314387 DB - PRIME DP - Unbound Medicine ER -