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Adenylate cyclase modulation of endocochlear potential during suppression of strial Na(+)-K+ ATPase.
Hear Res. 1992 Mar; 58(2):221-6.HR

Abstract

Forskolin, an adenylate cyclase activator, produces a reversible elevation of the endocochlear potential (EP) (Doi et al., 1990a). To determine whether strial Na(+)-K+ ATPase activity is essential for the forskolin-dependent EP elevation, we examined, by means of K(+)-selective microelectrodes, the effects of forskolin on the EP and the endolymphatic K+ activity ([K+]) while strial Na(+)-K+ ATPase was suppressed by ouabain. Perilymphatic perfusion with ouabain (10(-3) M) decreased the EP from 78.5 +/- 2.4 mV to -27.6 +/- 2.4 mV (N = 8) at 37.9 +/- 3.7 min after the start of perfusion and decreased the [K+] from 138.7 +/- 5.4 mM to 103.7 +/- 3.7 mM (N = 3). Successive perfusion with forskolin (2 x 10(-4) M) with ouabain (10(-3) M) increased the EP by 15.1 +/- 1.5 mV (N = 8) but did not influence the [K+] decrease from 101 +/- 3.6 mM to 95 +/- 1.3 mM (N = 3). Forskolin (2 x 10(-4) M) with ouabain (10(-3) M) without a preceding ouabain perfusion decreased the EP from 76.2 +/- 2.3 mV to -12.9 +/- 1.8 mV (N = 6) at 65.3 +/- 2.1 min after the start of perfusion. These results indicate that adenylate cyclase can modulate the EP in the absence of strial Na(+)-K+ ATPase activity and that adenylate cyclase activation can attenuate the EP drop induced by strial Na(+)-K+ ATPase suppression.

Authors+Show Affiliations

Department of Otolaryngology, Osaka University Medical School, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1314796

Citation

Doi, K, et al. "Adenylate Cyclase Modulation of Endocochlear Potential During Suppression of Strial Na(+)-K+ ATPase." Hearing Research, vol. 58, no. 2, 1992, pp. 221-6.
Doi K, Kitano I, Mori N. Adenylate cyclase modulation of endocochlear potential during suppression of strial Na(+)-K+ ATPase. Hear Res. 1992;58(2):221-6.
Doi, K., Kitano, I., & Mori, N. (1992). Adenylate cyclase modulation of endocochlear potential during suppression of strial Na(+)-K+ ATPase. Hearing Research, 58(2), 221-6.
Doi K, Kitano I, Mori N. Adenylate Cyclase Modulation of Endocochlear Potential During Suppression of Strial Na(+)-K+ ATPase. Hear Res. 1992;58(2):221-6. PubMed PMID: 1314796.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adenylate cyclase modulation of endocochlear potential during suppression of strial Na(+)-K+ ATPase. AU - Doi,K, AU - Kitano,I, AU - Mori,N, PY - 1992/3/1/pubmed PY - 1992/3/1/medline PY - 1992/3/1/entrez SP - 221 EP - 6 JF - Hearing research JO - Hear Res VL - 58 IS - 2 N2 - Forskolin, an adenylate cyclase activator, produces a reversible elevation of the endocochlear potential (EP) (Doi et al., 1990a). To determine whether strial Na(+)-K+ ATPase activity is essential for the forskolin-dependent EP elevation, we examined, by means of K(+)-selective microelectrodes, the effects of forskolin on the EP and the endolymphatic K+ activity ([K+]) while strial Na(+)-K+ ATPase was suppressed by ouabain. Perilymphatic perfusion with ouabain (10(-3) M) decreased the EP from 78.5 +/- 2.4 mV to -27.6 +/- 2.4 mV (N = 8) at 37.9 +/- 3.7 min after the start of perfusion and decreased the [K+] from 138.7 +/- 5.4 mM to 103.7 +/- 3.7 mM (N = 3). Successive perfusion with forskolin (2 x 10(-4) M) with ouabain (10(-3) M) increased the EP by 15.1 +/- 1.5 mV (N = 8) but did not influence the [K+] decrease from 101 +/- 3.6 mM to 95 +/- 1.3 mM (N = 3). Forskolin (2 x 10(-4) M) with ouabain (10(-3) M) without a preceding ouabain perfusion decreased the EP from 76.2 +/- 2.3 mV to -12.9 +/- 1.8 mV (N = 6) at 65.3 +/- 2.1 min after the start of perfusion. These results indicate that adenylate cyclase can modulate the EP in the absence of strial Na(+)-K+ ATPase activity and that adenylate cyclase activation can attenuate the EP drop induced by strial Na(+)-K+ ATPase suppression. SN - 0378-5955 UR - https://www.unboundmedicine.com/medline/citation/1314796/Adenylate_cyclase_modulation_of_endocochlear_potential_during_suppression_of_strial_Na_+__K+_ATPase_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0378-5955(92)90131-6 DB - PRIME DP - Unbound Medicine ER -