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A survey of Epstein-Barr virus gene expression in sporadic non-Hodgkin's lymphomas. Detection of Epstein-Barr virus in a subset of peripheral T-cell lymphomas.
Am J Pathol. 1992 Jun; 140(6):1315-25.AJ

Abstract

This study analyzes the association of Epstein-Barr virus (EBV) with non-Hodgkin's lymphoma (NHL) arising in patients without pre-existing overt immunodeficiency. The authors examined 201 lymphomas (105 high-grade B-cell, 82 peripheral T-cell, 7 high-grade non-B-cell, non-T-cell, and 7 hairy-cell leukemia) for EBV gene expression by immunohistologic procedures using monoclonal antibodies to EBV latent, immediate early, and replicative infection antigens. Transformation-associated EBV latent membrane protein 1 (LMP 1) was detected in 13 (6%) NHL, comprising 4 (4%) high-grade B-cell, 8 (10%) peripheral T-cell, and 1 non-B-cell, non-T-cell lymphomas. Anaplastic large-cell lymphoma of T-cell type was consistently LMP 1-negative. EBV nuclear antigen 2 was demonstrated in only three (1%) cases. Induction of replication as defined by expression of the immediate early BamHI Z leftward reading frame 1 (BZLF1) protein was detected in five cases, but early (EA) and late (VCA and MA) lytic cycle antigens were only found in two cases and in one case, respectively. The presence of EBV was confirmed by in situ DNA hybridization in 9 of 11 EBV antigen-positive lymphomas. This study shows the surprisingly frequent presence of EBV in peripheral T-cell NHL in European patients without pre-existing overt immunodeficiency. Interestingly, most sporadic B-cell NHL are not associated with the virus. Furthermore, the usefulness of selected monoclonal antibodies for the routine immunohistological diagnosis of EBV infection was confirmed.

Authors+Show Affiliations

Laboratory of immunohistology, Aarhus University Hospital, Denmark.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1318639

Citation

Hamilton-Dutoit, S J., and G Pallesen. "A Survey of Epstein-Barr Virus Gene Expression in Sporadic non-Hodgkin's Lymphomas. Detection of Epstein-Barr Virus in a Subset of Peripheral T-cell Lymphomas." The American Journal of Pathology, vol. 140, no. 6, 1992, pp. 1315-25.
Hamilton-Dutoit SJ, Pallesen G. A survey of Epstein-Barr virus gene expression in sporadic non-Hodgkin's lymphomas. Detection of Epstein-Barr virus in a subset of peripheral T-cell lymphomas. Am J Pathol. 1992;140(6):1315-25.
Hamilton-Dutoit, S. J., & Pallesen, G. (1992). A survey of Epstein-Barr virus gene expression in sporadic non-Hodgkin's lymphomas. Detection of Epstein-Barr virus in a subset of peripheral T-cell lymphomas. The American Journal of Pathology, 140(6), 1315-25.
Hamilton-Dutoit SJ, Pallesen G. A Survey of Epstein-Barr Virus Gene Expression in Sporadic non-Hodgkin's Lymphomas. Detection of Epstein-Barr Virus in a Subset of Peripheral T-cell Lymphomas. Am J Pathol. 1992;140(6):1315-25. PubMed PMID: 1318639.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A survey of Epstein-Barr virus gene expression in sporadic non-Hodgkin's lymphomas. Detection of Epstein-Barr virus in a subset of peripheral T-cell lymphomas. AU - Hamilton-Dutoit,S J, AU - Pallesen,G, PY - 1992/6/1/pubmed PY - 1992/6/1/medline PY - 1992/6/1/entrez SP - 1315 EP - 25 JF - The American journal of pathology JO - Am. J. Pathol. VL - 140 IS - 6 N2 - This study analyzes the association of Epstein-Barr virus (EBV) with non-Hodgkin's lymphoma (NHL) arising in patients without pre-existing overt immunodeficiency. The authors examined 201 lymphomas (105 high-grade B-cell, 82 peripheral T-cell, 7 high-grade non-B-cell, non-T-cell, and 7 hairy-cell leukemia) for EBV gene expression by immunohistologic procedures using monoclonal antibodies to EBV latent, immediate early, and replicative infection antigens. Transformation-associated EBV latent membrane protein 1 (LMP 1) was detected in 13 (6%) NHL, comprising 4 (4%) high-grade B-cell, 8 (10%) peripheral T-cell, and 1 non-B-cell, non-T-cell lymphomas. Anaplastic large-cell lymphoma of T-cell type was consistently LMP 1-negative. EBV nuclear antigen 2 was demonstrated in only three (1%) cases. Induction of replication as defined by expression of the immediate early BamHI Z leftward reading frame 1 (BZLF1) protein was detected in five cases, but early (EA) and late (VCA and MA) lytic cycle antigens were only found in two cases and in one case, respectively. The presence of EBV was confirmed by in situ DNA hybridization in 9 of 11 EBV antigen-positive lymphomas. This study shows the surprisingly frequent presence of EBV in peripheral T-cell NHL in European patients without pre-existing overt immunodeficiency. Interestingly, most sporadic B-cell NHL are not associated with the virus. Furthermore, the usefulness of selected monoclonal antibodies for the routine immunohistological diagnosis of EBV infection was confirmed. SN - 0002-9440 UR - https://www.unboundmedicine.com/medline/citation/1318639/A_survey_of_Epstein_Barr_virus_gene_expression_in_sporadic_non_Hodgkin's_lymphomas__Detection_of_Epstein_Barr_virus_in_a_subset_of_peripheral_T_cell_lymphomas_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/1318639/ DB - PRIME DP - Unbound Medicine ER -