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Mouse peritoneal macrophage prostaglandin E1 synthesis is altered by dietary gamma-linolenic acid.
J Nutr 1992; 122(8):1600-6JN

Abstract

The ability of dietary gamma-linolenic acid [18:3(n-6)] to modulate prostaglandin biosynthesis in mouse resident peritoneal macrophages was determined. Mice were fed diets containing corn oil, borage oil or evening primrose oil or a mixture of borage and fish oils. After 2 wk, resident peritoneal macrophages were isolated and stimulated with unopsonized zymosan to induce prostaglandin synthesis. Borage oil, primrose oil and fish-borage oil mixture dietary groups (containing 25.6, 11.9 and 19.5 g gamma-linolenic acid/100 g fatty acids, respectively) had significantly (P less than 0.05) enhanced prostaglandin E1 synthesis (39.7, 29.4 and 73.0 nmol prostaglandin E1/mg protein, respectively) compared with corn oil-fed (containing less than 0.1 g gamma-linolenic acid/100 g fatty acids) animals, which synthesized less than 0.1 nmol prostaglandin E1/mg protein. Borage oil- and fish-borage oil mixture-fed mice had the highest biosynthetic ratio of prostaglandin E1/prostaglandin E2 (E1/E2 approximately 0.2). Macrophages from borage oil-fed mice synthesized the lowest amount of prostacyclin (198.7 nmol 6-keto-prostaglandin F1 alpha/mg protein) compared with corn oil-, primrose oil- and fish-borage oil mixture-fed mice (379.7, 764.8 and 384.2 nmol 6-keto-prostaglandin F1 alpha/mg protein, respectively). In addition, borage oil-, primrose oil- and fish-borage oil mixture-fed mice had significantly (P less than 0.05) higher levels of dihomo-gamma-linolenic acid [20:3(n-6)] in membrane phospholipids (5.5, 3.5 and 5.7 mol/100 mol, respectively) relative to corn oil-fed mice (2.0 mol/100 mol).(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Department of Animal Science, Texas A&M University, College Station 77843-2471.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1322453

Citation

Fan, Y Y., and R S. Chapkin. "Mouse Peritoneal Macrophage Prostaglandin E1 Synthesis Is Altered By Dietary Gamma-linolenic Acid." The Journal of Nutrition, vol. 122, no. 8, 1992, pp. 1600-6.
Fan YY, Chapkin RS. Mouse peritoneal macrophage prostaglandin E1 synthesis is altered by dietary gamma-linolenic acid. J Nutr. 1992;122(8):1600-6.
Fan, Y. Y., & Chapkin, R. S. (1992). Mouse peritoneal macrophage prostaglandin E1 synthesis is altered by dietary gamma-linolenic acid. The Journal of Nutrition, 122(8), pp. 1600-6.
Fan YY, Chapkin RS. Mouse Peritoneal Macrophage Prostaglandin E1 Synthesis Is Altered By Dietary Gamma-linolenic Acid. J Nutr. 1992;122(8):1600-6. PubMed PMID: 1322453.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mouse peritoneal macrophage prostaglandin E1 synthesis is altered by dietary gamma-linolenic acid. AU - Fan,Y Y, AU - Chapkin,R S, PY - 1992/8/1/pubmed PY - 1992/8/1/medline PY - 1992/8/1/entrez SP - 1600 EP - 6 JF - The Journal of nutrition JO - J. Nutr. VL - 122 IS - 8 N2 - The ability of dietary gamma-linolenic acid [18:3(n-6)] to modulate prostaglandin biosynthesis in mouse resident peritoneal macrophages was determined. Mice were fed diets containing corn oil, borage oil or evening primrose oil or a mixture of borage and fish oils. After 2 wk, resident peritoneal macrophages were isolated and stimulated with unopsonized zymosan to induce prostaglandin synthesis. Borage oil, primrose oil and fish-borage oil mixture dietary groups (containing 25.6, 11.9 and 19.5 g gamma-linolenic acid/100 g fatty acids, respectively) had significantly (P less than 0.05) enhanced prostaglandin E1 synthesis (39.7, 29.4 and 73.0 nmol prostaglandin E1/mg protein, respectively) compared with corn oil-fed (containing less than 0.1 g gamma-linolenic acid/100 g fatty acids) animals, which synthesized less than 0.1 nmol prostaglandin E1/mg protein. Borage oil- and fish-borage oil mixture-fed mice had the highest biosynthetic ratio of prostaglandin E1/prostaglandin E2 (E1/E2 approximately 0.2). Macrophages from borage oil-fed mice synthesized the lowest amount of prostacyclin (198.7 nmol 6-keto-prostaglandin F1 alpha/mg protein) compared with corn oil-, primrose oil- and fish-borage oil mixture-fed mice (379.7, 764.8 and 384.2 nmol 6-keto-prostaglandin F1 alpha/mg protein, respectively). In addition, borage oil-, primrose oil- and fish-borage oil mixture-fed mice had significantly (P less than 0.05) higher levels of dihomo-gamma-linolenic acid [20:3(n-6)] in membrane phospholipids (5.5, 3.5 and 5.7 mol/100 mol, respectively) relative to corn oil-fed mice (2.0 mol/100 mol).(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0022-3166 UR - https://www.unboundmedicine.com/medline/citation/1322453/Mouse_peritoneal_macrophage_prostaglandin_E1_synthesis_is_altered_by_dietary_gamma_linolenic_acid_ L2 - https://academic.oup.com/jn/article-lookup/doi/10.1093/jn/122.8.1600 DB - PRIME DP - Unbound Medicine ER -