Simultaneous quantitation of delta-9-tetrahydrocannabinol (THC) and 11-nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in serum by GC/MS using deuterated internal standards and its application to a smoking study and forensic cases.
J Forensic Sci. 1992 Jul; 37(4):969-83.JF

Abstract

A new procedure for the simultaneous detection of delta-9-tetrahydrocannabinol (THC) and its major metabolite, 11-nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in serum has been evaluated. The method combines rapid, efficient, solid-phase extraction and simple derivatization by methylation. Analysis and quantitation is performed by gas chromatography/mass spectrometry (GC/MS) using deuterated cannabinoids as internal standards (IS). Reproducibility and sensitivity of the method are good. The procedure is applied to serum specimens collected from a smoking study with 24 volunteers and 212 forensic cases. Results are interpreted based upon the current knowledge about THC metabolism and pharmacokinetics.

Links

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Authors+Show Affiliations

Moeller MR

Institute of Legal Medicine, State University of Saarland, Homburg, Germany.

Doerr G

No affiliation info available

Warth S

No affiliation info available

MeSH

CalibrationDronabinolFemaleForensic MedicineGas Chromatography-Mass SpectrometryHalf-LifeHumansImmunoenzyme TechniquesMaleMarijuana SmokingReproducibility of Results

Pub Type(s)

Comparative Study

Journal Article

Language

eng

PubMed ID

1324293

Citation

Moeller, M R., et al. "Simultaneous Quantitation of Delta-9-tetrahydrocannabinol (THC) and 11-nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in Serum By GC/MS Using Deuterated Internal Standards and Its Application to a Smoking Study and Forensic Cases." Journal of Forensic Sciences, vol. 37, no. 4, 1992, pp. 969-83.

Moeller MR, Doerr G, Warth S. Simultaneous quantitation of delta-9-tetrahydrocannabinol (THC) and 11-nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in serum by GC/MS using deuterated internal standards and its application to a smoking study and forensic cases. J Forensic Sci. 1992;37(4):969-83.

Moeller, M. R., Doerr, G., & Warth, S. (1992). Simultaneous quantitation of delta-9-tetrahydrocannabinol (THC) and 11-nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in serum by GC/MS using deuterated internal standards and its application to a smoking study and forensic cases. Journal of Forensic Sciences, 37(4), 969-83.

Moeller MR, Doerr G, Warth S. Simultaneous Quantitation of Delta-9-tetrahydrocannabinol (THC) and 11-nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in Serum By GC/MS Using Deuterated Internal Standards and Its Application to a Smoking Study and Forensic Cases. J Forensic Sci. 1992;37(4):969-83. PubMed PMID: 1324293.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Simultaneous quantitation of delta-9-tetrahydrocannabinol (THC) and 11-nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in serum by GC/MS using deuterated internal standards and its application to a smoking study and forensic cases. AU - Moeller,M R, AU - Doerr,G, AU - Warth,S, PY - 1992/7/1/pubmed PY - 1992/7/1/medline PY - 1992/7/1/entrez SP - 969 EP - 83 JF - Journal of forensic sciences JO - J Forensic Sci VL - 37 IS - 4 N2 - A new procedure for the simultaneous detection of delta-9-tetrahydrocannabinol (THC) and its major metabolite, 11-nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in serum has been evaluated. The method combines rapid, efficient, solid-phase extraction and simple derivatization by methylation. Analysis and quantitation is performed by gas chromatography/mass spectrometry (GC/MS) using deuterated cannabinoids as internal standards (IS). Reproducibility and sensitivity of the method are good. The procedure is applied to serum specimens collected from a smoking study with 24 volunteers and 212 forensic cases. Results are interpreted based upon the current knowledge about THC metabolism and pharmacokinetics. SN - 0022-1198 UR - https://www.unboundmedicine.com/medline/citation/1324293/Simultaneous_quantitation_of_delta_9_tetrahydrocannabinol__THC__and_11_nor_9_carboxy_delta_9_tetrahydrocannabinol__THC_COOH__in_serum_by_GC/MS_using_deuterated_internal_standards_and_its_application_to_a_smoking_study_and_forensic_cases_ L2 - https://www.lens.org/lens/search/patent/list?q=citation_id:1324293 DB - PRIME DP - Unbound Medicine ER -

Tags

Simultaneous quantitation of delta-9-tetrahydrocannabinol (THC) and 11-nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in serum by GC/MS using deuterated internal standards and its application to a smoking study and forensic cases.J Forensic Sci. 1992 Jul; 37(4):969-83.JF