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High incidence of EBV genome in CD30-positive non-Hodgkin's lymphomas.
J Pathol. 1992 Nov; 168(3):307-15.JP

Abstract

In Hodgkin's disease, Epstein-Barr virus (EBV) is found in CD30-positive Reed-Sternberg cells. We therefore studied 60 CD30-positive non-Hodgkin's lymphomas (NHLs) for the presence of EBV by the polymerase chain reaction (PCR) and DNA in situ hybridization (DISH), and by immunohistochemistry for the latent EBV proteins LMP and EBNA-2. CD30-negative NHLs and reactive lymph nodes served as controls. The CD30-positive cases comprised 17 anaplastic large cell lymphomas (ALCLs) (> 75 per cent CD30-positive cells) and 43 non-ALCLs (with 5-35 per cent CD30-positive cells). By PCR, 40 of 60 CD30-positive NHLs (67 per cent) were EBV-positive; in CD30-negative cases, 6/29 (21 per cent) were EBV-positive, as were 12/50 (24 per cent) reactive lymph nodes. The DISH procedure demonstrated the EBV genome exclusively in the nuclei of tumour cells in 23 of the 37 PCR EBV-positive cases that were tested. PCR-negative cases were always DISH-negative, as were the PCR-positive reactive lymph nodes and CD30-negative NHLs. Immunohistochemistry demonstrated LMP in neoplastic cells of 7/47 (15 per cent) CD30-positive NHLs, both ALCL and non-ALCL always in PCR EBV-positive cases, but never in the two control groups. EBNA-2 staining could not be detected. It is concluded that EBV is present (and transcriptionally active) in a sizeable number of NHLs and an association between the presence of the EBV genome and CD30 expression seems likely.

Authors+Show Affiliations

Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1335046

Citation

Kanavaros, P, et al. "High Incidence of EBV Genome in CD30-positive non-Hodgkin's Lymphomas." The Journal of Pathology, vol. 168, no. 3, 1992, pp. 307-15.
Kanavaros P, Jiwa NM, de Bruin PC, et al. High incidence of EBV genome in CD30-positive non-Hodgkin's lymphomas. J Pathol. 1992;168(3):307-15.
Kanavaros, P., Jiwa, N. M., de Bruin, P. C., van der Valk, P., Noorduyn, L. A., van Heerde, P., Gordijn, R., Horstman, A., Mullink, R., & Willemze, R. (1992). High incidence of EBV genome in CD30-positive non-Hodgkin's lymphomas. The Journal of Pathology, 168(3), 307-15.
Kanavaros P, et al. High Incidence of EBV Genome in CD30-positive non-Hodgkin's Lymphomas. J Pathol. 1992;168(3):307-15. PubMed PMID: 1335046.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High incidence of EBV genome in CD30-positive non-Hodgkin's lymphomas. A1 - Kanavaros,P, AU - Jiwa,N M, AU - de Bruin,P C, AU - van der Valk,P, AU - Noorduyn,L A, AU - van Heerde,P, AU - Gordijn,R, AU - Horstman,A, AU - Mullink,R, AU - Willemze,R, PY - 1992/11/1/pubmed PY - 1992/11/1/medline PY - 1992/11/1/entrez SP - 307 EP - 15 JF - The Journal of pathology JO - J. Pathol. VL - 168 IS - 3 N2 - In Hodgkin's disease, Epstein-Barr virus (EBV) is found in CD30-positive Reed-Sternberg cells. We therefore studied 60 CD30-positive non-Hodgkin's lymphomas (NHLs) for the presence of EBV by the polymerase chain reaction (PCR) and DNA in situ hybridization (DISH), and by immunohistochemistry for the latent EBV proteins LMP and EBNA-2. CD30-negative NHLs and reactive lymph nodes served as controls. The CD30-positive cases comprised 17 anaplastic large cell lymphomas (ALCLs) (> 75 per cent CD30-positive cells) and 43 non-ALCLs (with 5-35 per cent CD30-positive cells). By PCR, 40 of 60 CD30-positive NHLs (67 per cent) were EBV-positive; in CD30-negative cases, 6/29 (21 per cent) were EBV-positive, as were 12/50 (24 per cent) reactive lymph nodes. The DISH procedure demonstrated the EBV genome exclusively in the nuclei of tumour cells in 23 of the 37 PCR EBV-positive cases that were tested. PCR-negative cases were always DISH-negative, as were the PCR-positive reactive lymph nodes and CD30-negative NHLs. Immunohistochemistry demonstrated LMP in neoplastic cells of 7/47 (15 per cent) CD30-positive NHLs, both ALCL and non-ALCL always in PCR EBV-positive cases, but never in the two control groups. EBNA-2 staining could not be detected. It is concluded that EBV is present (and transcriptionally active) in a sizeable number of NHLs and an association between the presence of the EBV genome and CD30 expression seems likely. SN - 0022-3417 UR - https://www.unboundmedicine.com/medline/citation/1335046/High_incidence_of_EBV_genome_in_CD30_positive_non_Hodgkin's_lymphomas_ L2 - https://doi.org/10.1002/path.1711680311 DB - PRIME DP - Unbound Medicine ER -