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Down-modulation of cell surface expression of p80 form of the tumor necrosis factor receptor by human immunodeficiency virus-1 tat gene.
Lymphokine Cytokine Res. 1992 Dec; 11(6):317-25.LC

Abstract

Tumor necrosis factor-alpha (TNF-alpha) induces the expression of human immunodeficiency virus type-1 (HIV-1) in vitro in chronically infected cells of T and monocytic origin. The tat protein from the HIV-1 virus has been shown to be essential for HIV replication and in the immunosuppression associated with the virus infection. Previous studies in our laboratory have shown that HIV-1 tat gene induces TNF-beta (lymphotoxin) in human B-lymphoblastoid cells (Sastry et al., 1990, J. Biol. Chem. 265, 20091-20093). In an attempt to characterize further the relationship between the host and HIV-1, we investigated the effect of the functional HIV-1 tat gene on the expression of TNF receptors in a human B lymphoblastoid cell line (Raji). We report here that Raji cells transfected with HIV-1 tat gene express fewer cell surface TNF receptors than control cells. At least a 5-fold decrease in the receptor number without any significant change in receptor affinity was observed. The decrease in TNF receptors in tat-transfected Raji cells (Raji-tat cells) was found not to be due to receptor occupancy by the autocrine production of TNF-beta. The decrease in the cell surface expression of TNF receptors in Raji-tat cells was also found to be not due to a decrease in the gene expression of the receptor. The kinetics, amount of TNF binding and its internalization were temperature dependent, and it was different in Raji-tat cells than in the control cells.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Department of Clinical Immunology and Biological Therapy, University of Texas M. D. Anderson Cancer, Houston 77030.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1335762

Citation

Pocsik, E, et al. "Down-modulation of Cell Surface Expression of P80 Form of the Tumor Necrosis Factor Receptor By Human Immunodeficiency Virus-1 Tat Gene." Lymphokine and Cytokine Research, vol. 11, no. 6, 1992, pp. 317-25.
Pocsik E, Higuchi M, Aggarwal BB. Down-modulation of cell surface expression of p80 form of the tumor necrosis factor receptor by human immunodeficiency virus-1 tat gene. Lymphokine Cytokine Res. 1992;11(6):317-25.
Pocsik, E., Higuchi, M., & Aggarwal, B. B. (1992). Down-modulation of cell surface expression of p80 form of the tumor necrosis factor receptor by human immunodeficiency virus-1 tat gene. Lymphokine and Cytokine Research, 11(6), 317-25.
Pocsik E, Higuchi M, Aggarwal BB. Down-modulation of Cell Surface Expression of P80 Form of the Tumor Necrosis Factor Receptor By Human Immunodeficiency Virus-1 Tat Gene. Lymphokine Cytokine Res. 1992;11(6):317-25. PubMed PMID: 1335762.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Down-modulation of cell surface expression of p80 form of the tumor necrosis factor receptor by human immunodeficiency virus-1 tat gene. AU - Pocsik,E, AU - Higuchi,M, AU - Aggarwal,B B, PY - 1992/12/1/pubmed PY - 1992/12/1/medline PY - 1992/12/1/entrez SP - 317 EP - 25 JF - Lymphokine and cytokine research JO - Lymphokine Cytokine Res. VL - 11 IS - 6 N2 - Tumor necrosis factor-alpha (TNF-alpha) induces the expression of human immunodeficiency virus type-1 (HIV-1) in vitro in chronically infected cells of T and monocytic origin. The tat protein from the HIV-1 virus has been shown to be essential for HIV replication and in the immunosuppression associated with the virus infection. Previous studies in our laboratory have shown that HIV-1 tat gene induces TNF-beta (lymphotoxin) in human B-lymphoblastoid cells (Sastry et al., 1990, J. Biol. Chem. 265, 20091-20093). In an attempt to characterize further the relationship between the host and HIV-1, we investigated the effect of the functional HIV-1 tat gene on the expression of TNF receptors in a human B lymphoblastoid cell line (Raji). We report here that Raji cells transfected with HIV-1 tat gene express fewer cell surface TNF receptors than control cells. At least a 5-fold decrease in the receptor number without any significant change in receptor affinity was observed. The decrease in TNF receptors in tat-transfected Raji cells (Raji-tat cells) was found not to be due to receptor occupancy by the autocrine production of TNF-beta. The decrease in the cell surface expression of TNF receptors in Raji-tat cells was also found to be not due to a decrease in the gene expression of the receptor. The kinetics, amount of TNF binding and its internalization were temperature dependent, and it was different in Raji-tat cells than in the control cells.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 1056-5477 UR - https://www.unboundmedicine.com/medline/citation/1335762/Down_modulation_of_cell_surface_expression_of_p80_form_of_the_tumor_necrosis_factor_receptor_by_human_immunodeficiency_virus_1_tat_gene_ DB - PRIME DP - Unbound Medicine ER -