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Characterization of [125I]Tyr11-somatostatin binding sites in the rabbit retina.
Neuropeptides. 1992 Jan; 21(1):13-9.N

Abstract

We have identified specific receptors for somatostatin (SS) in the rabbit retina using the radioligand [125I]Tyr11-Somatostatin. [125I]Tyr11-SS bound with high affinity to retinal membranes as was ascertained by both kinetic and saturation experiments. Scatchard analysis of the saturation data for [125I]Tyr11-SS binding to retinal membranes suggest a single population of sites with an apparent affinity constant (KD) of 0.90 +/- 0.20 nM and a maximum number of binding sites (Bmax) of 104 +/- 52 fmol/mg protein. The specific binding of [125I]Tyr11-SS was displaced in a dose-dependent manner by SS, Tyr11-SS, SMS 201-995, SS-28 and D-Trp8-SS. The inactive SS analog SS28(1-14) as well as the peptides CRF and bombesin had no effect. In addition, the specific binding of [125I]Tyr11-SS was attenuated by GTPgS. These findings demonstrate the presence of a selective receptor for SS in the rabbit retina that is coupled to guanine nucleotide binding protein(s).

Authors+Show Affiliations

Liapakis G
Department of Basic Sciences, School of Health Sciences, University of Crete, Heraklion, Greece.
Thermos K
No affiliation info available

MeSH

AnimalsBinding SitesIodine RadioisotopesMaleRabbitsReceptors, NeurotransmitterReceptors, SomatostatinRetinaSomatostatin

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1346715

Citation

Liapakis, G, and K Thermos. "Characterization of [125I]Tyr11-somatostatin Binding Sites in the Rabbit Retina." Neuropeptides, vol. 21, no. 1, 1992, pp. 13-9.
Liapakis G, Thermos K. Characterization of [125I]Tyr11-somatostatin binding sites in the rabbit retina. Neuropeptides. 1992;21(1):13-9.
Liapakis, G., & Thermos, K. (1992). Characterization of [125I]Tyr11-somatostatin binding sites in the rabbit retina. Neuropeptides, 21(1), 13-9.
Liapakis G, Thermos K. Characterization of [125I]Tyr11-somatostatin Binding Sites in the Rabbit Retina. Neuropeptides. 1992;21(1):13-9. PubMed PMID: 1346715.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of [125I]Tyr11-somatostatin binding sites in the rabbit retina. AU - Liapakis,G, AU - Thermos,K, PY - 1992/1/1/pubmed PY - 1992/1/1/medline PY - 1992/1/1/entrez SP - 13 EP - 9 JF - Neuropeptides JO - Neuropeptides VL - 21 IS - 1 N2 - We have identified specific receptors for somatostatin (SS) in the rabbit retina using the radioligand [125I]Tyr11-Somatostatin. [125I]Tyr11-SS bound with high affinity to retinal membranes as was ascertained by both kinetic and saturation experiments. Scatchard analysis of the saturation data for [125I]Tyr11-SS binding to retinal membranes suggest a single population of sites with an apparent affinity constant (KD) of 0.90 +/- 0.20 nM and a maximum number of binding sites (Bmax) of 104 +/- 52 fmol/mg protein. The specific binding of [125I]Tyr11-SS was displaced in a dose-dependent manner by SS, Tyr11-SS, SMS 201-995, SS-28 and D-Trp8-SS. The inactive SS analog SS28(1-14) as well as the peptides CRF and bombesin had no effect. In addition, the specific binding of [125I]Tyr11-SS was attenuated by GTPgS. These findings demonstrate the presence of a selective receptor for SS in the rabbit retina that is coupled to guanine nucleotide binding protein(s). SN - 0143-4179 UR - https://www.unboundmedicine.com/medline/citation/1346715/Characterization_of_[125I]Tyr11_somatostatin_binding_sites_in_the_rabbit_retina_ DB - PRIME DP - Unbound Medicine ER -