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In vitro induction of CD8 expression on thymic pre-T cells. I. Transforming growth factor-beta and tumor necrosis factor-alpha induce CD8 expression on CD8- thymic subsets including the CD25+CD3-CD4-CD8- pre-T cell subset.
J Immunol. 1992 Mar 15; 148(6):1737-45.JI

Abstract

We previously reported that IL-7 maintains the viability and differentiation potential of CD25 (IL-2R p55) positive CD3-CD4-CD8- thymic pre-T cells in vitro. This culture system is suitable for studying signals that regulate differentiation of T cell precursors in the thymus. In this study, we screened cytokines for their capacity to induce CD4 or CD8 in murine thymic pre-T cells cultured with IL-7. Of 15 cytokines tested, only transforming growth factor (TGF-beta) and TNF-alpha induced CD8 (Lyt-2), while no cytokine was able to induce CD4 on CD25+CD3-CD4-CD8- thymocytes. The combination of TGF-beta and TNF-alpha was synergistic, and the majority of cells recovered after 2 to 3 days in culture expressed CD8 (but not CD3 or CD4). A similar effect of TGF-beta and TNF-alpha was observed using day-15 fetal thymocytes, CD3+CD4-CD8- or CD3+CD4+CD8- adult thymocytes, although the combination of these cytokines resulted in an additive rather than a synergistic effect in these subsets. In contrast, neither TGF-beta nor TNF-alpha induced CD8 expression on splenic CD4+CD8- T cells. These observations suggest a role for these cytokines in the induction of CD8 expression in CD8- thymocyte subsets including CD3-CD4-CD8- thymic pre-T cells.

Authors+Show Affiliations

DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1347307

Citation

Suda, T, and A Zlotnik. "In Vitro Induction of CD8 Expression On Thymic pre-T Cells. I. Transforming Growth Factor-beta and Tumor Necrosis Factor-alpha Induce CD8 Expression On CD8- Thymic Subsets Including the CD25+CD3-CD4-CD8- pre-T Cell Subset." Journal of Immunology (Baltimore, Md. : 1950), vol. 148, no. 6, 1992, pp. 1737-45.
Suda T, Zlotnik A. In vitro induction of CD8 expression on thymic pre-T cells. I. Transforming growth factor-beta and tumor necrosis factor-alpha induce CD8 expression on CD8- thymic subsets including the CD25+CD3-CD4-CD8- pre-T cell subset. J Immunol. 1992;148(6):1737-45.
Suda, T., & Zlotnik, A. (1992). In vitro induction of CD8 expression on thymic pre-T cells. I. Transforming growth factor-beta and tumor necrosis factor-alpha induce CD8 expression on CD8- thymic subsets including the CD25+CD3-CD4-CD8- pre-T cell subset. Journal of Immunology (Baltimore, Md. : 1950), 148(6), 1737-45.
Suda T, Zlotnik A. In Vitro Induction of CD8 Expression On Thymic pre-T Cells. I. Transforming Growth Factor-beta and Tumor Necrosis Factor-alpha Induce CD8 Expression On CD8- Thymic Subsets Including the CD25+CD3-CD4-CD8- pre-T Cell Subset. J Immunol. 1992 Mar 15;148(6):1737-45. PubMed PMID: 1347307.
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TY - JOUR T1 - In vitro induction of CD8 expression on thymic pre-T cells. I. Transforming growth factor-beta and tumor necrosis factor-alpha induce CD8 expression on CD8- thymic subsets including the CD25+CD3-CD4-CD8- pre-T cell subset. AU - Suda,T, AU - Zlotnik,A, PY - 1992/3/15/pubmed PY - 1992/3/15/medline PY - 1992/3/15/entrez SP - 1737 EP - 45 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 148 IS - 6 N2 - We previously reported that IL-7 maintains the viability and differentiation potential of CD25 (IL-2R p55) positive CD3-CD4-CD8- thymic pre-T cells in vitro. This culture system is suitable for studying signals that regulate differentiation of T cell precursors in the thymus. In this study, we screened cytokines for their capacity to induce CD4 or CD8 in murine thymic pre-T cells cultured with IL-7. Of 15 cytokines tested, only transforming growth factor (TGF-beta) and TNF-alpha induced CD8 (Lyt-2), while no cytokine was able to induce CD4 on CD25+CD3-CD4-CD8- thymocytes. The combination of TGF-beta and TNF-alpha was synergistic, and the majority of cells recovered after 2 to 3 days in culture expressed CD8 (but not CD3 or CD4). A similar effect of TGF-beta and TNF-alpha was observed using day-15 fetal thymocytes, CD3+CD4-CD8- or CD3+CD4+CD8- adult thymocytes, although the combination of these cytokines resulted in an additive rather than a synergistic effect in these subsets. In contrast, neither TGF-beta nor TNF-alpha induced CD8 expression on splenic CD4+CD8- T cells. These observations suggest a role for these cytokines in the induction of CD8 expression in CD8- thymocyte subsets including CD3-CD4-CD8- thymic pre-T cells. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/1347307/In_vitro_induction_of_CD8_expression_on_thymic_pre_T_cells__I__Transforming_growth_factor_beta_and_tumor_necrosis_factor_alpha_induce_CD8_expression_on_CD8__thymic_subsets_including_the_CD25+CD3_CD4_CD8__pre_T_cell_subset_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=1347307 DB - PRIME DP - Unbound Medicine ER -