Tags

Type your tag names separated by a space and hit enter

Staurosporine induces a neuronal phenotype in SH-SY5Y human neuroblastoma cells that resembles that induced by the phorbol ester 12-O-tetradecanoyl phorbol-13 acetate (TPA).
FEBS Lett. 1992 Mar 30; 300(2):114-8.FL

Abstract

Treatment of SH-SY5Y human neuroblastoma cells with the protein kinase inhibitor staurosporine, induced both morphological and functional differentiation in these cells. The effects of staurosporine were comparable to those induced by the protein kinase C (PKC) activator, 12-O-tetradecanoyl phorbol 13-acetate (TPA), with respect to induction of neuronal differentiation, i.e. neurite outgrowth, inhibition of DNA synthesis, induction and down-regulation of c-myc protein expression, induction of mRNA for both neuropeptide Y (NPY) and growth associated protein 43 (GAP-43) and stimulation of tyrosine hydroxylase expression. Staurosporine failed to translocate PKC to the membrane fraction or to stimulate phosphorylation of the endogenous PKC substrate M(r) 80,000 (p80). Instead, staurosporine inhibited TPA-induced phosphorylation of p80.

Authors+Show Affiliations

Department of Biochemistry and Pharmacy, Abo Akademi University, Turku, Finland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1348695

Citation

Jalava, A, et al. "Staurosporine Induces a Neuronal Phenotype in SH-SY5Y Human Neuroblastoma Cells That Resembles That Induced By the Phorbol Ester 12-O-tetradecanoyl Phorbol-13 Acetate (TPA)." FEBS Letters, vol. 300, no. 2, 1992, pp. 114-8.
Jalava A, Heikkilä J, Lintunen M, et al. Staurosporine induces a neuronal phenotype in SH-SY5Y human neuroblastoma cells that resembles that induced by the phorbol ester 12-O-tetradecanoyl phorbol-13 acetate (TPA). FEBS Lett. 1992;300(2):114-8.
Jalava, A., Heikkilä, J., Lintunen, M., Akerman, K., & Påhlman, S. (1992). Staurosporine induces a neuronal phenotype in SH-SY5Y human neuroblastoma cells that resembles that induced by the phorbol ester 12-O-tetradecanoyl phorbol-13 acetate (TPA). FEBS Letters, 300(2), 114-8.
Jalava A, et al. Staurosporine Induces a Neuronal Phenotype in SH-SY5Y Human Neuroblastoma Cells That Resembles That Induced By the Phorbol Ester 12-O-tetradecanoyl Phorbol-13 Acetate (TPA). FEBS Lett. 1992 Mar 30;300(2):114-8. PubMed PMID: 1348695.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Staurosporine induces a neuronal phenotype in SH-SY5Y human neuroblastoma cells that resembles that induced by the phorbol ester 12-O-tetradecanoyl phorbol-13 acetate (TPA). AU - Jalava,A, AU - Heikkilä,J, AU - Lintunen,M, AU - Akerman,K, AU - Påhlman,S, PY - 1992/3/30/pubmed PY - 1992/3/30/medline PY - 1992/3/30/entrez SP - 114 EP - 8 JF - FEBS letters JO - FEBS Lett VL - 300 IS - 2 N2 - Treatment of SH-SY5Y human neuroblastoma cells with the protein kinase inhibitor staurosporine, induced both morphological and functional differentiation in these cells. The effects of staurosporine were comparable to those induced by the protein kinase C (PKC) activator, 12-O-tetradecanoyl phorbol 13-acetate (TPA), with respect to induction of neuronal differentiation, i.e. neurite outgrowth, inhibition of DNA synthesis, induction and down-regulation of c-myc protein expression, induction of mRNA for both neuropeptide Y (NPY) and growth associated protein 43 (GAP-43) and stimulation of tyrosine hydroxylase expression. Staurosporine failed to translocate PKC to the membrane fraction or to stimulate phosphorylation of the endogenous PKC substrate M(r) 80,000 (p80). Instead, staurosporine inhibited TPA-induced phosphorylation of p80. SN - 0014-5793 UR - https://www.unboundmedicine.com/medline/citation/1348695/Staurosporine_induces_a_neuronal_phenotype_in_SH_SY5Y_human_neuroblastoma_cells_that_resembles_that_induced_by_the_phorbol_ester_12_O_tetradecanoyl_phorbol_13_acetate__TPA__ L2 - https://linkinghub.elsevier.com/retrieve/pii/0014-5793(92)80176-H DB - PRIME DP - Unbound Medicine ER -