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Release of vasoactive intestinal polypeptide from the rat gastric fundus.
Br J Pharmacol. 1992 Mar; 105(3):691-5.BJ

Abstract

1. Auxotonic responses and release of vasoactive intestinal polypeptide-like immunoreactivity (VIP-LI) induced by electrical field stimulation (EFS) were studied in longitudinal muscle strips from the gastric fundus of reserpinized rats suspended between parallel platinum electrodes in Krebs solution containing atropine (1 microM), 5-hydroxytryptamine (3 microM) and bovine serum albumin (50 mg l-1). 2. EFS (supramaximal voltage, 1 ms, 0.25-32.0 Hz, trains of 2 min) induced frequency-dependent relaxations. 3. EFS at frequencies greater than or equal to 8 Hz also produced significant increases in VIP-LI release. 4. VIP-LI release induced by EFS at 16 Hz no longer occurred in the presence of tetrodotoxin (1 microM) or a Ca(2+)-free medium. 5. Detection of VIP-LI upon activation of inhibitory non-adrenergic, non-cholinergic neurones indicates that VIP meets the 'detectable release' criterion for an inhibitory neurotransmitter in the rat gastric fundus.

Authors+Show Affiliations

Institute of Pharmacology, Catholic University School of Medicine, Rome, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1352721

Citation

D'Amato, M, et al. "Release of Vasoactive Intestinal Polypeptide From the Rat Gastric Fundus." British Journal of Pharmacology, vol. 105, no. 3, 1992, pp. 691-5.
D'Amato M, Currò D, Montuschi P, et al. Release of vasoactive intestinal polypeptide from the rat gastric fundus. Br J Pharmacol. 1992;105(3):691-5.
D'Amato, M., Currò, D., Montuschi, P., Ciabattoni, G., Ragazzoni, E., & Lefebvre, R. A. (1992). Release of vasoactive intestinal polypeptide from the rat gastric fundus. British Journal of Pharmacology, 105(3), 691-5.
D'Amato M, et al. Release of Vasoactive Intestinal Polypeptide From the Rat Gastric Fundus. Br J Pharmacol. 1992;105(3):691-5. PubMed PMID: 1352721.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Release of vasoactive intestinal polypeptide from the rat gastric fundus. AU - D'Amato,M, AU - Currò,D, AU - Montuschi,P, AU - Ciabattoni,G, AU - Ragazzoni,E, AU - Lefebvre,R A, PY - 1992/3/1/pubmed PY - 1992/3/1/medline PY - 1992/3/1/entrez SP - 691 EP - 5 JF - British journal of pharmacology JO - Br. J. Pharmacol. VL - 105 IS - 3 N2 - 1. Auxotonic responses and release of vasoactive intestinal polypeptide-like immunoreactivity (VIP-LI) induced by electrical field stimulation (EFS) were studied in longitudinal muscle strips from the gastric fundus of reserpinized rats suspended between parallel platinum electrodes in Krebs solution containing atropine (1 microM), 5-hydroxytryptamine (3 microM) and bovine serum albumin (50 mg l-1). 2. EFS (supramaximal voltage, 1 ms, 0.25-32.0 Hz, trains of 2 min) induced frequency-dependent relaxations. 3. EFS at frequencies greater than or equal to 8 Hz also produced significant increases in VIP-LI release. 4. VIP-LI release induced by EFS at 16 Hz no longer occurred in the presence of tetrodotoxin (1 microM) or a Ca(2+)-free medium. 5. Detection of VIP-LI upon activation of inhibitory non-adrenergic, non-cholinergic neurones indicates that VIP meets the 'detectable release' criterion for an inhibitory neurotransmitter in the rat gastric fundus. SN - 0007-1188 UR - https://www.unboundmedicine.com/medline/citation/1352721/Release_of_vasoactive_intestinal_polypeptide_from_the_rat_gastric_fundus_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0007-1188&date=1992&volume=105&issue=3&spage=691 DB - PRIME DP - Unbound Medicine ER -