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Evidence for protein kinase-C mediation of the neurotensin-induced activation of tyrosine hydroxylase in tuberoinfundibular dopaminergic neurons.
Endocrinology. 1992 Sep; 131(3):1207-11.E

Abstract

The purpose of the present study was to determine whether neurotensin acts within the arcuate nucleus/median eminence to activate tyrosine hydroxylase (TH) within tuberoinfundibular dopamine neurons. The role of Ca2+/phospholipid-dependent protein kinase (protein kinase-C) in the regulation of TH and its involvement in the neurotensin-induced activation of TH within tuberoinfundibular dopamine (TIDA) neurons also was investigated. The activity of TH within TIDA neurons was assessed by quantification of the formation of 3,4-dihydroxyphenylalanine in the arcuate nucleus/median eminence after inhibition of 3,4-dihydroxyphenylalanine decarboxylase. Neurotensin (0.1-10 nM) increased the activity of TH within the arcuate nucleus/median eminence under in vitro conditions by approximately 80%. The activity of TH in the arcuate nucleus/median eminence also was increased approximately 55% by the phorbol ester 12-O-tetradecanoyl(phorbol-13-acetate) (1-100 nM), which activates protein kinase-C. Sphingosine (10 microM), an inhibitor of protein kinase-C, attenuated the activation of TH within TIDA neurons that was induced by both 12-O-tetradecanoyl(phorbol-13-acetate) and neurotensin. Sphingosine alone did not alter the activity of TH, nor did it alter the (Bu)2cAMP-induced activation of TH in the arcuate nucleus/median eminence. It is concluded that neurotensin acts directly within the arcuate nucleus/median eminence to activate TIDA neurons. Furthermore, it is suggested that the activity of TH within these neurons is enhanced after the activation of protein kinase-C and that protein kinase-C may mediate the neurotensin-induced activation of TH within these hypothalamic dopamine neurons.

Authors+Show Affiliations

Berry SA
Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio 44106.
Gudelsky GA
No affiliation info available

MeSH

AnimalsArcuate Nucleus of HypothalamusBucladesineDihydroxyphenylalanineDopamineEnzyme ActivationHydrazinesHypothalamusIn Vitro TechniquesMaleMedian EminenceNeuronsNeurotensinProtein Kinase CRatsRats, Inbred StrainsSphingosineTetradecanoylphorbol AcetateTyrosine 3-Monooxygenase

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1354601

Citation

Berry, S A., and G A. Gudelsky. "Evidence for Protein kinase-C Mediation of the Neurotensin-induced Activation of Tyrosine Hydroxylase in Tuberoinfundibular Dopaminergic Neurons." Endocrinology, vol. 131, no. 3, 1992, pp. 1207-11.
Berry SA, Gudelsky GA. Evidence for protein kinase-C mediation of the neurotensin-induced activation of tyrosine hydroxylase in tuberoinfundibular dopaminergic neurons. Endocrinology. 1992;131(3):1207-11.
Berry, S. A., & Gudelsky, G. A. (1992). Evidence for protein kinase-C mediation of the neurotensin-induced activation of tyrosine hydroxylase in tuberoinfundibular dopaminergic neurons. Endocrinology, 131(3), 1207-11.
Berry SA, Gudelsky GA. Evidence for Protein kinase-C Mediation of the Neurotensin-induced Activation of Tyrosine Hydroxylase in Tuberoinfundibular Dopaminergic Neurons. Endocrinology. 1992;131(3):1207-11. PubMed PMID: 1354601.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence for protein kinase-C mediation of the neurotensin-induced activation of tyrosine hydroxylase in tuberoinfundibular dopaminergic neurons. AU - Berry,S A, AU - Gudelsky,G A, PY - 1992/9/1/pubmed PY - 1992/9/1/medline PY - 1992/9/1/entrez SP - 1207 EP - 11 JF - Endocrinology JO - Endocrinology VL - 131 IS - 3 N2 - The purpose of the present study was to determine whether neurotensin acts within the arcuate nucleus/median eminence to activate tyrosine hydroxylase (TH) within tuberoinfundibular dopamine neurons. The role of Ca2+/phospholipid-dependent protein kinase (protein kinase-C) in the regulation of TH and its involvement in the neurotensin-induced activation of TH within tuberoinfundibular dopamine (TIDA) neurons also was investigated. The activity of TH within TIDA neurons was assessed by quantification of the formation of 3,4-dihydroxyphenylalanine in the arcuate nucleus/median eminence after inhibition of 3,4-dihydroxyphenylalanine decarboxylase. Neurotensin (0.1-10 nM) increased the activity of TH within the arcuate nucleus/median eminence under in vitro conditions by approximately 80%. The activity of TH in the arcuate nucleus/median eminence also was increased approximately 55% by the phorbol ester 12-O-tetradecanoyl(phorbol-13-acetate) (1-100 nM), which activates protein kinase-C. Sphingosine (10 microM), an inhibitor of protein kinase-C, attenuated the activation of TH within TIDA neurons that was induced by both 12-O-tetradecanoyl(phorbol-13-acetate) and neurotensin. Sphingosine alone did not alter the activity of TH, nor did it alter the (Bu)2cAMP-induced activation of TH in the arcuate nucleus/median eminence. It is concluded that neurotensin acts directly within the arcuate nucleus/median eminence to activate TIDA neurons. Furthermore, it is suggested that the activity of TH within these neurons is enhanced after the activation of protein kinase-C and that protein kinase-C may mediate the neurotensin-induced activation of TH within these hypothalamic dopamine neurons. SN - 0013-7227 UR - https://www.unboundmedicine.com/medline/citation/1354601/Evidence_for_protein_kinase_C_mediation_of_the_neurotensin_induced_activation_of_tyrosine_hydroxylase_in_tuberoinfundibular_dopaminergic_neurons_ DB - PRIME DP - Unbound Medicine ER -