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Sensitive determination of phenothiazines in body fluids by gas chromatography with surface ionization detection.
J Chromatogr. 1992 Sep 02; 579(2):247-52.JC

Abstract

Fourteen phenothiazine derivatives were tested for their detection by gas chromatography (GC) with surface ionization detection (SID). The sensitivity of GC-SID was highest with trimeprazine and levomepromazine, which contain aliphatic tertiary amino side chains, and lowest with thiethylperazine and thioproperazine, which contain sulphur residues. Chlorpromazine, trimeprazine and promazine showed excellent linearity between the SID response and the drug amount in the range 0.25-3.0 pmol on-column. Their detection limits were as low as ca. 5-10 pg (15-30 fmol) on-column (250-500 pg per ml of body fluid). A detailed procedure for isolation of phenothiazines from human whole blood and urine using Sep-Pak C18 cartridges, before the GC with SID, is also presented. The recoveries of the drugs (100 pmol), which were added to 1 ml of whole blood or urine, were more than 79%. The baselines remained steady as the column temperature was increased.

Authors+Show Affiliations

Department of Legal Medicine, Aichi Medical University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1358904

Citation

Hattori, H, et al. "Sensitive Determination of Phenothiazines in Body Fluids By Gas Chromatography With Surface Ionization Detection." Journal of Chromatography, vol. 579, no. 2, 1992, pp. 247-52.
Hattori H, Yamamoto S, Iwata M, et al. Sensitive determination of phenothiazines in body fluids by gas chromatography with surface ionization detection. J Chromatogr. 1992;579(2):247-52.
Hattori, H., Yamamoto, S., Iwata, M., Takashima, E., Yamada, T., & Suzuki, O. (1992). Sensitive determination of phenothiazines in body fluids by gas chromatography with surface ionization detection. Journal of Chromatography, 579(2), 247-52.
Hattori H, et al. Sensitive Determination of Phenothiazines in Body Fluids By Gas Chromatography With Surface Ionization Detection. J Chromatogr. 1992 Sep 2;579(2):247-52. PubMed PMID: 1358904.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sensitive determination of phenothiazines in body fluids by gas chromatography with surface ionization detection. AU - Hattori,H, AU - Yamamoto,S, AU - Iwata,M, AU - Takashima,E, AU - Yamada,T, AU - Suzuki,O, PY - 1992/9/2/pubmed PY - 1992/9/2/medline PY - 1992/9/2/entrez SP - 247 EP - 52 JF - Journal of chromatography JO - J Chromatogr VL - 579 IS - 2 N2 - Fourteen phenothiazine derivatives were tested for their detection by gas chromatography (GC) with surface ionization detection (SID). The sensitivity of GC-SID was highest with trimeprazine and levomepromazine, which contain aliphatic tertiary amino side chains, and lowest with thiethylperazine and thioproperazine, which contain sulphur residues. Chlorpromazine, trimeprazine and promazine showed excellent linearity between the SID response and the drug amount in the range 0.25-3.0 pmol on-column. Their detection limits were as low as ca. 5-10 pg (15-30 fmol) on-column (250-500 pg per ml of body fluid). A detailed procedure for isolation of phenothiazines from human whole blood and urine using Sep-Pak C18 cartridges, before the GC with SID, is also presented. The recoveries of the drugs (100 pmol), which were added to 1 ml of whole blood or urine, were more than 79%. The baselines remained steady as the column temperature was increased. UR - https://www.unboundmedicine.com/medline/citation/1358904/Sensitive_determination_of_phenothiazines_in_body_fluids_by_gas_chromatography_with_surface_ionization_detection_ DB - PRIME DP - Unbound Medicine ER -