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Determination of diphenylmethane antihistaminic drugs and their analogues in body fluids by gas chromatography with surface ionization detection.
J Chromatogr. 1992 Oct 23; 581(2):213-8.JC

Abstract

Eleven diphenylmethane antihistaminic drugs and their analogues were tested for their detection by capillary gas chromatography (GC) with surface ionization detection (SID). The GC-SID response was highest for doxylamine, diphenhydramine and orphenadrine and lowest for terodiline, clemastine and pipethanate. The detection limits for drugs with the highest response were 2-5 pg (ca. 6-20 fmol) on-column (100-250 pg/ml of body fluid). The detection limits with GC-SID were 10-100 times higher than those with GC with nitrogen-phosphorus detection. A detailed procedure for the isolation of the antihistaminics from human whole blood and urine by the use of Sep-Pak C18 cartridges, prior to GC-SID, is also presented. The recoveries of the drugs (50 or 500 pmol), which had been added to 1 ml of body fluids, were > 60%. The baselines remained steady as the column temperature was increased and the background was clean, especially for whole blood extracts.

Authors+Show Affiliations

Department of Legal Medicine, Aichi Medical University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1360473

Citation

Hattori, H, et al. "Determination of Diphenylmethane Antihistaminic Drugs and Their Analogues in Body Fluids By Gas Chromatography With Surface Ionization Detection." Journal of Chromatography, vol. 581, no. 2, 1992, pp. 213-8.
Hattori H, Yamamoto S, Iwata M, et al. Determination of diphenylmethane antihistaminic drugs and their analogues in body fluids by gas chromatography with surface ionization detection. J Chromatogr. 1992;581(2):213-8.
Hattori, H., Yamamoto, S., Iwata, M., Takashima, E., Yamada, T., & Suzuki, O. (1992). Determination of diphenylmethane antihistaminic drugs and their analogues in body fluids by gas chromatography with surface ionization detection. Journal of Chromatography, 581(2), 213-8.
Hattori H, et al. Determination of Diphenylmethane Antihistaminic Drugs and Their Analogues in Body Fluids By Gas Chromatography With Surface Ionization Detection. J Chromatogr. 1992 Oct 23;581(2):213-8. PubMed PMID: 1360473.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of diphenylmethane antihistaminic drugs and their analogues in body fluids by gas chromatography with surface ionization detection. AU - Hattori,H, AU - Yamamoto,S, AU - Iwata,M, AU - Takashima,E, AU - Yamada,T, AU - Suzuki,O, PY - 1992/10/23/pubmed PY - 1992/10/23/medline PY - 1992/10/23/entrez SP - 213 EP - 8 JF - Journal of chromatography JO - J Chromatogr VL - 581 IS - 2 N2 - Eleven diphenylmethane antihistaminic drugs and their analogues were tested for their detection by capillary gas chromatography (GC) with surface ionization detection (SID). The GC-SID response was highest for doxylamine, diphenhydramine and orphenadrine and lowest for terodiline, clemastine and pipethanate. The detection limits for drugs with the highest response were 2-5 pg (ca. 6-20 fmol) on-column (100-250 pg/ml of body fluid). The detection limits with GC-SID were 10-100 times higher than those with GC with nitrogen-phosphorus detection. A detailed procedure for the isolation of the antihistaminics from human whole blood and urine by the use of Sep-Pak C18 cartridges, prior to GC-SID, is also presented. The recoveries of the drugs (50 or 500 pmol), which had been added to 1 ml of body fluids, were > 60%. The baselines remained steady as the column temperature was increased and the background was clean, especially for whole blood extracts. UR - https://www.unboundmedicine.com/medline/citation/1360473/Determination_of_diphenylmethane_antihistaminic_drugs_and_their_analogues_in_body_fluids_by_gas_chromatography_with_surface_ionization_detection_ L2 - http://RD3FS2PT9J.search.serialssolutions.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pqm&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&genre=article&issn=&eissn=&volume=581&issue=2&spage=213&date=1992 DB - PRIME DP - Unbound Medicine ER -