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Rapid screening for taxanes by tandem mass spectrometry.
Anal Chem. 1992 Oct 15; 64(20):2313-5.AC

Abstract

A highly specific and sensitive method is described for determining taxol, cephalomannine, and baccatin III in crude plant extracts. Radical anions of the taxanes are formed by desorption chemical ionization, and a parent tandem mass spectrometric scan is used to recognize these compounds by their characteristic dissociations. The limit of detection of the individual taxanes in typical plant matrices is less than 500 pg when all three species are screened simultaneously. Because of the sensitivity of the method, extraction times can be shortened to 30 min and crude extracts can be examined at the rate of 6/h. Detection of all three taxanes extracted from a single Taxus cuspidata needle in a combined extraction/analysis time of less than 1 h is demonstrated.

Authors+Show Affiliations

Department of Chemistry, Purdue University, West Lafayette, Indiana 47907-1393.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1361307

Citation

Hoke, S H., et al. "Rapid Screening for Taxanes By Tandem Mass Spectrometry." Analytical Chemistry, vol. 64, no. 20, 1992, pp. 2313-5.
Hoke SH, Wood JM, Cooks RG, et al. Rapid screening for taxanes by tandem mass spectrometry. Anal Chem. 1992;64(20):2313-5.
Hoke, S. H., Wood, J. M., Cooks, R. G., Li, X. H., & Chang, C. J. (1992). Rapid screening for taxanes by tandem mass spectrometry. Analytical Chemistry, 64(20), 2313-5.
Hoke SH, et al. Rapid Screening for Taxanes By Tandem Mass Spectrometry. Anal Chem. 1992 Oct 15;64(20):2313-5. PubMed PMID: 1361307.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid screening for taxanes by tandem mass spectrometry. AU - Hoke,S H,2nd AU - Wood,J M, AU - Cooks,R G, AU - Li,X H, AU - Chang,C J, PY - 1992/10/15/pubmed PY - 1992/10/15/medline PY - 1992/10/15/entrez SP - 2313 EP - 5 JF - Analytical chemistry JO - Anal Chem VL - 64 IS - 20 N2 - A highly specific and sensitive method is described for determining taxol, cephalomannine, and baccatin III in crude plant extracts. Radical anions of the taxanes are formed by desorption chemical ionization, and a parent tandem mass spectrometric scan is used to recognize these compounds by their characteristic dissociations. The limit of detection of the individual taxanes in typical plant matrices is less than 500 pg when all three species are screened simultaneously. Because of the sensitivity of the method, extraction times can be shortened to 30 min and crude extracts can be examined at the rate of 6/h. Detection of all three taxanes extracted from a single Taxus cuspidata needle in a combined extraction/analysis time of less than 1 h is demonstrated. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/1361307/Rapid_screening_for_taxanes_by_tandem_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -