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Inhibitory NANC nerves in human tracheal smooth muscle: a quest for the neurotransmitter.
J Appl Physiol (1985). 1992 Dec; 73(6):2505-10.JA

Abstract

Inhibitory nonadrenergic noncholinergic (i-NANC) nerves are the only neural bronchodilator pathway in human airways. Possible candidates for the neurotransmitter include vasoactive intestinal peptide (VIP) and nitric oxide (NO) and purines such as ATP. We have investigated the potential role of these neurotransmitters. Phosphoramidon (10(-5) M) significantly potentiated relaxations to low doses of VIP with no effect on i-NANC responses. Relaxations induced by VIp were abolished with alpha-chymotrypsin (2 U/ml), but i-NANC responses were unaffected. Reactive blue 2 had no effect on i-NANC neural responses, indicating that endogenous ATP was not involved. The NO synthase inhibitor L-NG-nitroarginine methyl ester (L-NAME, 10(-4) M) produced a concentration-dependent inhibition of the i-NANC response, producing almost complete inhibition at every frequency studied (0.5-40 Hz), whereas L-NG-monomethyl arginine was effective only at low stimulation frequencies. The inhibitory effect of L-NAME was partially reversed by L- but not D-arginine, and D-NAME was without effect. These results suggest that in human tracheal segments the neural bronchodilator response is mediated by NO, and there is no functional evidence for implicating VIP in this response.

Authors+Show Affiliations

Department of Thoracic Medicine, National Heart and Lung Institute, London, United Kingdom.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1362724

Citation

Belvisi, M G., et al. "Inhibitory NANC Nerves in Human Tracheal Smooth Muscle: a Quest for the Neurotransmitter." Journal of Applied Physiology (Bethesda, Md. : 1985), vol. 73, no. 6, 1992, pp. 2505-10.
Belvisi MG, Stretton CD, Miura M, et al. Inhibitory NANC nerves in human tracheal smooth muscle: a quest for the neurotransmitter. J Appl Physiol (1985). 1992;73(6):2505-10.
Belvisi, M. G., Stretton, C. D., Miura, M., Verleden, G. M., Tadjkarimi, S., Yacoub, M. H., & Barnes, P. J. (1992). Inhibitory NANC nerves in human tracheal smooth muscle: a quest for the neurotransmitter. Journal of Applied Physiology (Bethesda, Md. : 1985), 73(6), 2505-10.
Belvisi MG, et al. Inhibitory NANC Nerves in Human Tracheal Smooth Muscle: a Quest for the Neurotransmitter. J Appl Physiol (1985). 1992;73(6):2505-10. PubMed PMID: 1362724.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory NANC nerves in human tracheal smooth muscle: a quest for the neurotransmitter. AU - Belvisi,M G, AU - Stretton,C D, AU - Miura,M, AU - Verleden,G M, AU - Tadjkarimi,S, AU - Yacoub,M H, AU - Barnes,P J, PY - 1992/12/1/pubmed PY - 1992/12/1/medline PY - 1992/12/1/entrez SP - 2505 EP - 10 JF - Journal of applied physiology (Bethesda, Md. : 1985) JO - J Appl Physiol (1985) VL - 73 IS - 6 N2 - Inhibitory nonadrenergic noncholinergic (i-NANC) nerves are the only neural bronchodilator pathway in human airways. Possible candidates for the neurotransmitter include vasoactive intestinal peptide (VIP) and nitric oxide (NO) and purines such as ATP. We have investigated the potential role of these neurotransmitters. Phosphoramidon (10(-5) M) significantly potentiated relaxations to low doses of VIP with no effect on i-NANC responses. Relaxations induced by VIp were abolished with alpha-chymotrypsin (2 U/ml), but i-NANC responses were unaffected. Reactive blue 2 had no effect on i-NANC neural responses, indicating that endogenous ATP was not involved. The NO synthase inhibitor L-NG-nitroarginine methyl ester (L-NAME, 10(-4) M) produced a concentration-dependent inhibition of the i-NANC response, producing almost complete inhibition at every frequency studied (0.5-40 Hz), whereas L-NG-monomethyl arginine was effective only at low stimulation frequencies. The inhibitory effect of L-NAME was partially reversed by L- but not D-arginine, and D-NAME was without effect. These results suggest that in human tracheal segments the neural bronchodilator response is mediated by NO, and there is no functional evidence for implicating VIP in this response. SN - 8750-7587 UR - https://www.unboundmedicine.com/medline/citation/1362724/Inhibitory_NANC_nerves_in_human_tracheal_smooth_muscle:_a_quest_for_the_neurotransmitter_ L2 - https://journals.physiology.org/doi/10.1152/jappl.1992.73.6.2505?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -