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Characterization of p40 and IL-10 in the BALF of patients with pulmonary sarcoidosis.
J Interferon Cytokine Res. 2003 Aug; 23(8):449-56.JI

Abstract

This study investigated cytokine protein levels (interleukin-12 p70 [IL-12p70], p40, and IL-10) in bronchoalveolar lavage fluid (BALF) from patients with pulmonary sarcoidosis (n = 59), healthy control subjects (n = 17), and patients with idiopathic pulmonary fibrosis (IPF) (n = 30). The relationship between cytokine levels and clinical course of sarcoidosis was also examined. Overall, p40 was far more abundant than IL-12p70. p40 levels (pg/ml, mean +/- SEM) were significantly higher in the BALF from patients with sarcoidosis (2.97 +/- 3.69) than in IPF patients (0.83 +/- 1.57) and healthy subjects (0.78 +/- 1.00). Size exclusion chromatography indicated that p40 detected in BALF from sarcoidosis patients corresponded to p40 monomers or (p40)(2) homodimers. Further, p40 levels were associated with (paralleled) the clinical course of sarcoidosis, with the highest levels detected in BALF from patients with persistent disease. Higher p40 levels were also found in the BALF from sarcoid patients who required corticosteroid treatment compared with patients with spontaneous regression (3.51 +/- 3.83 vs. 2.01 +/- 3.43, p = 0.03). IL-10 concentrations paralleled p40 changes. No similar association was found for IL-12p70 levels. In conclusion, this report shows that the BALF from patients with sarcoidosis contains elevated levels of p40, (p40)(2), and IL-10 protein but not of IL-12p70. The present data also suggest that BALF p40 concentrations may be indicative of the sarcoidosis clinical course.

Authors+Show Affiliations

Industrial Toxicology and Occupational Medicine Unit, Université Catholique de Louvain, Brussels, Belgium. virginie.barbarin@toxi.ucl.ac.beNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

13678433

Citation

Barbarin, Virginie, et al. "Characterization of P40 and IL-10 in the BALF of Patients With Pulmonary Sarcoidosis." Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research, vol. 23, no. 8, 2003, pp. 449-56.
Barbarin V, Petrek M, Kolek V, et al. Characterization of p40 and IL-10 in the BALF of patients with pulmonary sarcoidosis. J Interferon Cytokine Res. 2003;23(8):449-56.
Barbarin, V., Petrek, M., Kolek, V., Van Snick, J., Huaux, F., & Lison, D. (2003). Characterization of p40 and IL-10 in the BALF of patients with pulmonary sarcoidosis. Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research, 23(8), 449-56.
Barbarin V, et al. Characterization of P40 and IL-10 in the BALF of Patients With Pulmonary Sarcoidosis. J Interferon Cytokine Res. 2003;23(8):449-56. PubMed PMID: 13678433.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of p40 and IL-10 in the BALF of patients with pulmonary sarcoidosis. AU - Barbarin,Virginie, AU - Petrek,Martin, AU - Kolek,Vitezslav, AU - Van Snick,Jacques, AU - Huaux,François, AU - Lison,Dominique, PY - 2003/9/19/pubmed PY - 2004/3/18/medline PY - 2003/9/19/entrez SP - 449 EP - 56 JF - Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research JO - J Interferon Cytokine Res VL - 23 IS - 8 N2 - This study investigated cytokine protein levels (interleukin-12 p70 [IL-12p70], p40, and IL-10) in bronchoalveolar lavage fluid (BALF) from patients with pulmonary sarcoidosis (n = 59), healthy control subjects (n = 17), and patients with idiopathic pulmonary fibrosis (IPF) (n = 30). The relationship between cytokine levels and clinical course of sarcoidosis was also examined. Overall, p40 was far more abundant than IL-12p70. p40 levels (pg/ml, mean +/- SEM) were significantly higher in the BALF from patients with sarcoidosis (2.97 +/- 3.69) than in IPF patients (0.83 +/- 1.57) and healthy subjects (0.78 +/- 1.00). Size exclusion chromatography indicated that p40 detected in BALF from sarcoidosis patients corresponded to p40 monomers or (p40)(2) homodimers. Further, p40 levels were associated with (paralleled) the clinical course of sarcoidosis, with the highest levels detected in BALF from patients with persistent disease. Higher p40 levels were also found in the BALF from sarcoid patients who required corticosteroid treatment compared with patients with spontaneous regression (3.51 +/- 3.83 vs. 2.01 +/- 3.43, p = 0.03). IL-10 concentrations paralleled p40 changes. No similar association was found for IL-12p70 levels. In conclusion, this report shows that the BALF from patients with sarcoidosis contains elevated levels of p40, (p40)(2), and IL-10 protein but not of IL-12p70. The present data also suggest that BALF p40 concentrations may be indicative of the sarcoidosis clinical course. SN - 1079-9907 UR - https://www.unboundmedicine.com/medline/citation/13678433/Characterization_of_p40_and_IL_10_in_the_BALF_of_patients_with_pulmonary_sarcoidosis_ L2 - https://www.liebertpub.com/doi/10.1089/107999003322277865?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -