Tags

Type your tag names separated by a space and hit enter

HMW and LMW glutenin alleles among putative tetraploid and hexaploid European spelt wheat (Triticum spelta L.) progenitors.
Theor Appl Genet. 2003 Nov; 107(7):1321-30.TA

Abstract

The allelic compositions of high- and low-molecular-weight subunits of glutenins (HMW-GS and LMW-GS) among European spelt (Triticum spelta L.) and related hexaploid and tetraploid Triticum species were investigated by one- and two-dimensional polyacrylamide-gel electrophoresis (PAGE) and capillary electrophoresis (CE). A total of seven novel glutenin alleles (designated A1a*, B1d*, B1g*, B1f*, B1j*, D1a* at Glu-1 and A3h at the Glu-3 loci, respectively) in European spelt wheat were detected by SDS-PAGE, which were confirmed further by employing A-PAGE and CE methods. Particularly, two HMW-GS alleles, Glu-B1d* coding the subunits 6.1 and 22.1, and Glu-B1f* coding the subunits 13 and 22*, were found to occur in European spelt with frequencies of 32.34% and 5.11%, respectively. These two alleles were present in cultivated emmer (Triticum dicoccum), but they were not observed in bread wheat (Triticum aestivum L.). The allele Glu-B1g* coding for 13* and 19* subunits found in spelt wheat was also detected in club wheat (Triticum compactum L.). Additionally, two alleles coding for LMW-GS, Glu-A3h and Glu-B3d, occurred with high frequencies in spelt, club and cultivated emmer wheat, whereas these were not found or present with very low frequencies in bread wheat. Our results strongly support the secondary origin hypothesis, namely European spelt wheat originated from hybridization between cultivated emmer and club wheat. This is also confirmed experimentally by the artificial synthesis of spelt through crossing between old European emmer wheat, T. dicoccum and club wheat, T. compactum.

Authors+Show Affiliations

Technical University of Munich, Division of Plant Breeding and Applied Genetics, D-85350 Freising-Weihenstephan, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

13679994

Citation

Yan, Y, et al. "HMW and LMW Glutenin Alleles Among Putative Tetraploid and Hexaploid European Spelt Wheat (Triticum Spelta L.) Progenitors." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 107, no. 7, 2003, pp. 1321-30.
Yan Y, Hsam SL, Yu JZ, et al. HMW and LMW glutenin alleles among putative tetraploid and hexaploid European spelt wheat (Triticum spelta L.) progenitors. Theor Appl Genet. 2003;107(7):1321-30.
Yan, Y., Hsam, S. L., Yu, J. Z., Jiang, Y., Ohtsuka, I., & Zeller, F. J. (2003). HMW and LMW glutenin alleles among putative tetraploid and hexaploid European spelt wheat (Triticum spelta L.) progenitors. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 107(7), 1321-30.
Yan Y, et al. HMW and LMW Glutenin Alleles Among Putative Tetraploid and Hexaploid European Spelt Wheat (Triticum Spelta L.) Progenitors. Theor Appl Genet. 2003;107(7):1321-30. PubMed PMID: 13679994.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HMW and LMW glutenin alleles among putative tetraploid and hexaploid European spelt wheat (Triticum spelta L.) progenitors. AU - Yan,Y, AU - Hsam,S L K, AU - Yu,J Z, AU - Jiang,Y, AU - Ohtsuka,I, AU - Zeller,F J, Y1 - 2003/09/13/ PY - 2003/01/25/received PY - 2003/03/31/accepted PY - 2003/9/19/pubmed PY - 2004/2/18/medline PY - 2003/9/19/entrez SP - 1321 EP - 30 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 107 IS - 7 N2 - The allelic compositions of high- and low-molecular-weight subunits of glutenins (HMW-GS and LMW-GS) among European spelt (Triticum spelta L.) and related hexaploid and tetraploid Triticum species were investigated by one- and two-dimensional polyacrylamide-gel electrophoresis (PAGE) and capillary electrophoresis (CE). A total of seven novel glutenin alleles (designated A1a*, B1d*, B1g*, B1f*, B1j*, D1a* at Glu-1 and A3h at the Glu-3 loci, respectively) in European spelt wheat were detected by SDS-PAGE, which were confirmed further by employing A-PAGE and CE methods. Particularly, two HMW-GS alleles, Glu-B1d* coding the subunits 6.1 and 22.1, and Glu-B1f* coding the subunits 13 and 22*, were found to occur in European spelt with frequencies of 32.34% and 5.11%, respectively. These two alleles were present in cultivated emmer (Triticum dicoccum), but they were not observed in bread wheat (Triticum aestivum L.). The allele Glu-B1g* coding for 13* and 19* subunits found in spelt wheat was also detected in club wheat (Triticum compactum L.). Additionally, two alleles coding for LMW-GS, Glu-A3h and Glu-B3d, occurred with high frequencies in spelt, club and cultivated emmer wheat, whereas these were not found or present with very low frequencies in bread wheat. Our results strongly support the secondary origin hypothesis, namely European spelt wheat originated from hybridization between cultivated emmer and club wheat. This is also confirmed experimentally by the artificial synthesis of spelt through crossing between old European emmer wheat, T. dicoccum and club wheat, T. compactum. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/13679994/HMW_and_LMW_glutenin_alleles_among_putative_tetraploid_and_hexaploid_European_spelt_wheat__Triticum_spelta_L___progenitors_ L2 - https://dx.doi.org/10.1007/s00122-003-1315-z DB - PRIME DP - Unbound Medicine ER -