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The in vitro effect of aqueous extract of Nigella sativa seeds on nitric oxide production.
Phytother Res. 2003 Sep; 17(8):921-4.PR

Abstract

The in vitro effect of aqueous extract of Nigella sativa seeds on nitric oxide (NO) production by murine macrophages was studied. Murine peritoneal macrophages were pre-incubated with the extract and then activated with Escherichia coli lipopolysaccharride. NO production was measured after 24 hours by spectrophotometry. The plant extract caused a dose-dependent decrease in NO production. Dialyzed preparation of the extract did not affect NO production. However, the boiled fraction of the extract resulted in a dose-dependent inhibition of NO apparently comparable to that of the whole extract. These results indicate that the aqueous extract of N. sativa seeds exhibits an inhibitory effect on nitric oxide production by murine macrophages and the active component(s) is/are non-protein in nature. In view of the fact that nitric oxide is a pro-inflammatory mediator, this study validates the traditional use of the Nigella sativa seeds for the treatment of rheumatism.

Authors+Show Affiliations

Department of Biological and Biomedical Sciences, The Aga Khan University Medical College, Karachi, Pakistan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

13680825

Citation

Mahmood, M Saeed, et al. "The in Vitro Effect of Aqueous Extract of Nigella Sativa Seeds On Nitric Oxide Production." Phytotherapy Research : PTR, vol. 17, no. 8, 2003, pp. 921-4.
Mahmood MS, Gilani AH, Khwaja A, et al. The in vitro effect of aqueous extract of Nigella sativa seeds on nitric oxide production. Phytother Res. 2003;17(8):921-4.
Mahmood, M. S., Gilani, A. H., Khwaja, A., Rashid, A., & Ashfaq, M. K. (2003). The in vitro effect of aqueous extract of Nigella sativa seeds on nitric oxide production. Phytotherapy Research : PTR, 17(8), 921-4.
Mahmood MS, et al. The in Vitro Effect of Aqueous Extract of Nigella Sativa Seeds On Nitric Oxide Production. Phytother Res. 2003;17(8):921-4. PubMed PMID: 13680825.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The in vitro effect of aqueous extract of Nigella sativa seeds on nitric oxide production. AU - Mahmood,M Saeed, AU - Gilani,A H, AU - Khwaja,Afsheen, AU - Rashid,Ayesha, AU - Ashfaq,M K, PY - 2003/9/19/pubmed PY - 2003/12/3/medline PY - 2003/9/19/entrez SP - 921 EP - 4 JF - Phytotherapy research : PTR JO - Phytother Res VL - 17 IS - 8 N2 - The in vitro effect of aqueous extract of Nigella sativa seeds on nitric oxide (NO) production by murine macrophages was studied. Murine peritoneal macrophages were pre-incubated with the extract and then activated with Escherichia coli lipopolysaccharride. NO production was measured after 24 hours by spectrophotometry. The plant extract caused a dose-dependent decrease in NO production. Dialyzed preparation of the extract did not affect NO production. However, the boiled fraction of the extract resulted in a dose-dependent inhibition of NO apparently comparable to that of the whole extract. These results indicate that the aqueous extract of N. sativa seeds exhibits an inhibitory effect on nitric oxide production by murine macrophages and the active component(s) is/are non-protein in nature. In view of the fact that nitric oxide is a pro-inflammatory mediator, this study validates the traditional use of the Nigella sativa seeds for the treatment of rheumatism. SN - 0951-418X UR - https://www.unboundmedicine.com/medline/citation/13680825/The_in_vitro_effect_of_aqueous_extract_of_Nigella_sativa_seeds_on_nitric_oxide_production_ L2 - https://doi.org/10.1002/ptr.1251 DB - PRIME DP - Unbound Medicine ER -