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Isolation, characterization, and antitumor activities of the cell wall polysaccharides from Elsinoe leucospila.
Biosci Biotechnol Biochem. 1992 Jan; 56(1):29-33.BB

Abstract

A cell-wall preparation from the cells of Elsinoe leucospila, which produces elsinan extracellularly when grown on sucrose or glucose-potato extract medium, was fractionated systematically. The heteropolysaccharide that was released by treatment with Actinase E digestion, comprised D-mannose, D-galactose, and D-glucose (molar ratio, 1.5:1.0:0.1). Methylation, mild acid hydrolysis, and 13C-NMR studies suggested that the polysaccharide contains a backbone of alpha-(1----6)-linked D-mannose residues having two kinds of side chains, one attached at the O-4 with single or short beta-(1----6)-linked D-galactofuranosyl residues, and the other attached at O-2 with short side chains, most probably, of alpha-(1----3)-linked D-mannopyranosyl residues. A moderately branched D-glucan fraction, obtained from the cold alkali extract, was fractionated to give an antitumor-active purified beta-(1----3)-glucan having branches of single beta-D-glucosyl groups, one out of eight D-glucose residues being substituted at the O-6.

Authors+Show Affiliations

Department of Food and Nutrition, Faculty of Science of Living, Osaka City University, Japan.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1368133

Citation

Shirasugi, N, and A Misaki. "Isolation, Characterization, and Antitumor Activities of the Cell Wall Polysaccharides From Elsinoe Leucospila." Bioscience, Biotechnology, and Biochemistry, vol. 56, no. 1, 1992, pp. 29-33.
Shirasugi N, Misaki A. Isolation, characterization, and antitumor activities of the cell wall polysaccharides from Elsinoe leucospila. Biosci Biotechnol Biochem. 1992;56(1):29-33.
Shirasugi, N., & Misaki, A. (1992). Isolation, characterization, and antitumor activities of the cell wall polysaccharides from Elsinoe leucospila. Bioscience, Biotechnology, and Biochemistry, 56(1), 29-33.
Shirasugi N, Misaki A. Isolation, Characterization, and Antitumor Activities of the Cell Wall Polysaccharides From Elsinoe Leucospila. Biosci Biotechnol Biochem. 1992;56(1):29-33. PubMed PMID: 1368133.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation, characterization, and antitumor activities of the cell wall polysaccharides from Elsinoe leucospila. AU - Shirasugi,N, AU - Misaki,A, PY - 1992/1/1/pubmed PY - 1992/1/1/medline PY - 1992/1/1/entrez SP - 29 EP - 33 JF - Bioscience, biotechnology, and biochemistry JO - Biosci Biotechnol Biochem VL - 56 IS - 1 N2 - A cell-wall preparation from the cells of Elsinoe leucospila, which produces elsinan extracellularly when grown on sucrose or glucose-potato extract medium, was fractionated systematically. The heteropolysaccharide that was released by treatment with Actinase E digestion, comprised D-mannose, D-galactose, and D-glucose (molar ratio, 1.5:1.0:0.1). Methylation, mild acid hydrolysis, and 13C-NMR studies suggested that the polysaccharide contains a backbone of alpha-(1----6)-linked D-mannose residues having two kinds of side chains, one attached at the O-4 with single or short beta-(1----6)-linked D-galactofuranosyl residues, and the other attached at O-2 with short side chains, most probably, of alpha-(1----3)-linked D-mannopyranosyl residues. A moderately branched D-glucan fraction, obtained from the cold alkali extract, was fractionated to give an antitumor-active purified beta-(1----3)-glucan having branches of single beta-D-glucosyl groups, one out of eight D-glucose residues being substituted at the O-6. SN - 0916-8451 UR - https://www.unboundmedicine.com/medline/citation/1368133/Isolation_characterization_and_antitumor_activities_of_the_cell_wall_polysaccharides_from_Elsinoe_leucospila_ DB - PRIME DP - Unbound Medicine ER -