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Shuttling of pre-mRNA binding proteins between nucleus and cytoplasm.
Nature. 1992 Feb 20; 355(6362):730-2.Nat

Abstract

RNA polymerase II transcripts, heterogeneous nuclear RNAs (hnRNAs), associate in the nucleus with specific proteins that bind premessenger RNA (hnRNP proteins) and with small nuclear ribonucleoprotein particles (snRNPs). These hnRNA-hnRNP-snRNP complexes assemble on nascent transcripts and hnRNA is processed to mRNA in them. HnRNP proteins have been localized to the nucleoplasm and their functions were presumed to be limited to nuclear events in mRNA biogenesis. It was proposed that an exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. We show here that several of the abundant hnRNP proteins, including A1, shuttle between the nucleus and the cytoplasm. HnRNP proteins may thus also have cytoplasmic functions. Furthermore, when in the cytoplasm, A1 is bound to mRNA and RNA polymerase II transcription is necessary before it can return to the nucleus. We propose that the cytoplasmic ribonucleoprotein complex of mRNA with hnRNP proteins is the substrate of nuclear-cytoplasmic transport of mRNA.

Authors+Show Affiliations

Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104-6148.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1371331

Citation

Piñol-Roma, S, and G Dreyfuss. "Shuttling of pre-mRNA Binding Proteins Between Nucleus and Cytoplasm." Nature, vol. 355, no. 6362, 1992, pp. 730-2.
Piñol-Roma S, Dreyfuss G. Shuttling of pre-mRNA binding proteins between nucleus and cytoplasm. Nature. 1992;355(6362):730-2.
Piñol-Roma, S., & Dreyfuss, G. (1992). Shuttling of pre-mRNA binding proteins between nucleus and cytoplasm. Nature, 355(6362), 730-2.
Piñol-Roma S, Dreyfuss G. Shuttling of pre-mRNA Binding Proteins Between Nucleus and Cytoplasm. Nature. 1992 Feb 20;355(6362):730-2. PubMed PMID: 1371331.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Shuttling of pre-mRNA binding proteins between nucleus and cytoplasm. AU - Piñol-Roma,S, AU - Dreyfuss,G, PY - 1992/2/20/pubmed PY - 1992/2/20/medline PY - 1992/2/20/entrez SP - 730 EP - 2 JF - Nature JO - Nature VL - 355 IS - 6362 N2 - RNA polymerase II transcripts, heterogeneous nuclear RNAs (hnRNAs), associate in the nucleus with specific proteins that bind premessenger RNA (hnRNP proteins) and with small nuclear ribonucleoprotein particles (snRNPs). These hnRNA-hnRNP-snRNP complexes assemble on nascent transcripts and hnRNA is processed to mRNA in them. HnRNP proteins have been localized to the nucleoplasm and their functions were presumed to be limited to nuclear events in mRNA biogenesis. It was proposed that an exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. We show here that several of the abundant hnRNP proteins, including A1, shuttle between the nucleus and the cytoplasm. HnRNP proteins may thus also have cytoplasmic functions. Furthermore, when in the cytoplasm, A1 is bound to mRNA and RNA polymerase II transcription is necessary before it can return to the nucleus. We propose that the cytoplasmic ribonucleoprotein complex of mRNA with hnRNP proteins is the substrate of nuclear-cytoplasmic transport of mRNA. SN - 0028-0836 UR - https://www.unboundmedicine.com/medline/citation/1371331/Shuttling_of_pre_mRNA_binding_proteins_between_nucleus_and_cytoplasm_ L2 - https://doi.org/10.1038/355730a0 DB - PRIME DP - Unbound Medicine ER -