Tags

Type your tag names separated by a space and hit enter

Protoporphyrin and long-wave ultraviolet light modulate metabolic events in rat peritoneal mast cells.
J Invest Dermatol. 1992 Apr; 98(4):488-93.JI

Abstract

We have previously shown that protoporphyrin (PP) plus long-wave ultraviolet light (UVA) has an inhibitory effect on the release of histamine from rat peritoneal mast cells in response to various stimuli, without compromising cell viability. In the present study, we observed that protoporphyrin at a noncytolytic dose (3 ng/ml) plus UVA irradiation (0.038 J/cm2) is also able to suppress prostaglandin D2 generation by rat peritoneal mast cells in response to calcium ionophore A23187, compound 48/80, or anti-IgE antibody by 64%, 92%, and 100%, respectively. Because of the participation of protein kinase C in stimulus-secretion coupling in mast cells, we also investigated the effect of PP plus UVA on the release of histamine induced by the protein kinase C activator, phorbol 12-myristate 13-acetate (PMA). PP plus UVA inhibited histamine release induced by PMA. The release of histamine induced by the synergistic combination of PMA (50 nM) and a low dose of calcium ionophore A23187 (0.1 microM) was also inhibited. PP plus UVA inhibited the release of histamine induced by the non-fluorescent calcium ionophore, 4-Br-A23187, by 47.8%, but had essentially no effect on changes in intracellular calcium induced by this stimulus. In contrast, both the release of histamine and changes in intracellular calcium stimulated by compound 48/80 were inhibited. We conclude from these results that PP plus UVA may affect both early and late biochemical events involved in mast cell mediator release.

Authors+Show Affiliations

Yen A
Department of Medicine, University of California, School of Medicine, San Diego 92103-8420.
Barrett KE
No affiliation info available
Gigli I
No affiliation info available

MeSH

AnimalsCalcimycinCalciumCytosolHistamine ReleaseMaleMast CellsPeritoneal CavityProstaglandin D2ProtoporphyrinsRatsRats, Inbred StrainsTetradecanoylphorbol AcetateUltraviolet Raysp-Methoxy-N-methylphenethylamine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1372341

Citation

Yen, A, et al. "Protoporphyrin and Long-wave Ultraviolet Light Modulate Metabolic Events in Rat Peritoneal Mast Cells." The Journal of Investigative Dermatology, vol. 98, no. 4, 1992, pp. 488-93.
Yen A, Barrett KE, Gigli I. Protoporphyrin and long-wave ultraviolet light modulate metabolic events in rat peritoneal mast cells. J Invest Dermatol. 1992;98(4):488-93.
Yen, A., Barrett, K. E., & Gigli, I. (1992). Protoporphyrin and long-wave ultraviolet light modulate metabolic events in rat peritoneal mast cells. The Journal of Investigative Dermatology, 98(4), 488-93.
Yen A, Barrett KE, Gigli I. Protoporphyrin and Long-wave Ultraviolet Light Modulate Metabolic Events in Rat Peritoneal Mast Cells. J Invest Dermatol. 1992;98(4):488-93. PubMed PMID: 1372341.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protoporphyrin and long-wave ultraviolet light modulate metabolic events in rat peritoneal mast cells. AU - Yen,A, AU - Barrett,K E, AU - Gigli,I, PY - 1992/4/1/pubmed PY - 1992/4/1/medline PY - 1992/4/1/entrez SP - 488 EP - 93 JF - The Journal of investigative dermatology JO - J Invest Dermatol VL - 98 IS - 4 N2 - We have previously shown that protoporphyrin (PP) plus long-wave ultraviolet light (UVA) has an inhibitory effect on the release of histamine from rat peritoneal mast cells in response to various stimuli, without compromising cell viability. In the present study, we observed that protoporphyrin at a noncytolytic dose (3 ng/ml) plus UVA irradiation (0.038 J/cm2) is also able to suppress prostaglandin D2 generation by rat peritoneal mast cells in response to calcium ionophore A23187, compound 48/80, or anti-IgE antibody by 64%, 92%, and 100%, respectively. Because of the participation of protein kinase C in stimulus-secretion coupling in mast cells, we also investigated the effect of PP plus UVA on the release of histamine induced by the protein kinase C activator, phorbol 12-myristate 13-acetate (PMA). PP plus UVA inhibited histamine release induced by PMA. The release of histamine induced by the synergistic combination of PMA (50 nM) and a low dose of calcium ionophore A23187 (0.1 microM) was also inhibited. PP plus UVA inhibited the release of histamine induced by the non-fluorescent calcium ionophore, 4-Br-A23187, by 47.8%, but had essentially no effect on changes in intracellular calcium induced by this stimulus. In contrast, both the release of histamine and changes in intracellular calcium stimulated by compound 48/80 were inhibited. We conclude from these results that PP plus UVA may affect both early and late biochemical events involved in mast cell mediator release. SN - 0022-202X UR - https://www.unboundmedicine.com/medline/citation/1372341/Protoporphyrin_and_long_wave_ultraviolet_light_modulate_metabolic_events_in_rat_peritoneal_mast_cells_ DB - PRIME DP - Unbound Medicine ER -