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Relative proportions of pathogen-related oral spirochetes (PROS) and Treponema denticola in supragingival and subgingival plaque from patients with periodontitis.
J Periodontol. 1992 Feb; 63(2):131-6.JP

Abstract

The purpose of this study was to use monoclonal antibodies to enumerate spirochetes in dental plaque, including the newly recognized pathogen-related oral spirochete (PROS) and specific serovars of Treponema denticola. Plaque was collected from control subjects with no apparent periodontal disease and from sites of moderate to severe chronic periodontitis in patients with inflammatory periodontal disease. Individual monoclonal antibodies were used to determine whether spirochetes were present and then a double-staining protocol was employed to count total spirochetes and specific treponemes in individual microscopic fields. Results indicate that spirochetes are more common at diseased sites and in subgingival plaque than at healthy sites or in supragingival plaque. Together PROS and T. denticola comprised the majority of all spirochetes in all samples and PROS and T. denticola serovars "B" and D were most numerous in plaque from patients with periodontitis. PROS were the majority of all spirochetes in supragingival plaque (76.2% +/- 23.8%) and subgingival plaque (60.9% +/- 19.1%) from periodontitis patients, significantly larger than the percentage of T. denticola serovar "B" (P less than .001 for both supragingival and subgingival plaque) and serovar D (P less than .01 for supragingival and P less than .001 for subgingival plaque). These observations indicate that PROS are the predominant spirochete in plaque from sites of patients with periodontitis, but other analytical approaches are necessary to determine if PROS or T. denticola are pathogenic.

Authors+Show Affiliations

Department of Pediatric Dentistry, Oregon Health Sciences University, Portland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1372653

Citation

Riviere, G R., et al. "Relative Proportions of Pathogen-related Oral Spirochetes (PROS) and Treponema Denticola in Supragingival and Subgingival Plaque From Patients With Periodontitis." Journal of Periodontology, vol. 63, no. 2, 1992, pp. 131-6.
Riviere GR, Elliot KS, Adams DF, et al. Relative proportions of pathogen-related oral spirochetes (PROS) and Treponema denticola in supragingival and subgingival plaque from patients with periodontitis. J Periodontol. 1992;63(2):131-6.
Riviere, G. R., Elliot, K. S., Adams, D. F., Simonson, L. G., Forgas, L. B., Nilius, A. M., & Lukehart, S. A. (1992). Relative proportions of pathogen-related oral spirochetes (PROS) and Treponema denticola in supragingival and subgingival plaque from patients with periodontitis. Journal of Periodontology, 63(2), 131-6.
Riviere GR, et al. Relative Proportions of Pathogen-related Oral Spirochetes (PROS) and Treponema Denticola in Supragingival and Subgingival Plaque From Patients With Periodontitis. J Periodontol. 1992;63(2):131-6. PubMed PMID: 1372653.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relative proportions of pathogen-related oral spirochetes (PROS) and Treponema denticola in supragingival and subgingival plaque from patients with periodontitis. AU - Riviere,G R, AU - Elliot,K S, AU - Adams,D F, AU - Simonson,L G, AU - Forgas,L B, AU - Nilius,A M, AU - Lukehart,S A, PY - 1992/2/1/pubmed PY - 1992/2/1/medline PY - 1992/2/1/entrez SP - 131 EP - 6 JF - Journal of periodontology JO - J. Periodontol. VL - 63 IS - 2 N2 - The purpose of this study was to use monoclonal antibodies to enumerate spirochetes in dental plaque, including the newly recognized pathogen-related oral spirochete (PROS) and specific serovars of Treponema denticola. Plaque was collected from control subjects with no apparent periodontal disease and from sites of moderate to severe chronic periodontitis in patients with inflammatory periodontal disease. Individual monoclonal antibodies were used to determine whether spirochetes were present and then a double-staining protocol was employed to count total spirochetes and specific treponemes in individual microscopic fields. Results indicate that spirochetes are more common at diseased sites and in subgingival plaque than at healthy sites or in supragingival plaque. Together PROS and T. denticola comprised the majority of all spirochetes in all samples and PROS and T. denticola serovars "B" and D were most numerous in plaque from patients with periodontitis. PROS were the majority of all spirochetes in supragingival plaque (76.2% +/- 23.8%) and subgingival plaque (60.9% +/- 19.1%) from periodontitis patients, significantly larger than the percentage of T. denticola serovar "B" (P less than .001 for both supragingival and subgingival plaque) and serovar D (P less than .01 for supragingival and P less than .001 for subgingival plaque). These observations indicate that PROS are the predominant spirochete in plaque from sites of patients with periodontitis, but other analytical approaches are necessary to determine if PROS or T. denticola are pathogenic. SN - 0022-3492 UR - https://www.unboundmedicine.com/medline/citation/1372653/Relative_proportions_of_pathogen_related_oral_spirochetes__PROS__and_Treponema_denticola_in_supragingival_and_subgingival_plaque_from_patients_with_periodontitis_ L2 - https://doi.org/10.1902/jop.1992.63.2.131 DB - PRIME DP - Unbound Medicine ER -