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The micronucleus assay with peripheral blood reticulocytes by acridine orange supravital staining with 1-beta-D-arabinofuranosylcytosine.
Mutat Res. 1992 Feb-Mar; 278(2-3):131-7.MR

Abstract

Dose-dependent induction of micronuclei with 1-beta-D-arabinofuranosylcytosine (ara-C) was clearly shown in CD-1 mouse peripheral blood reticulocytes (RETs) using an acridine orange (AO) supravital staining method, as well as in the conventional bone marrow assay. The maximum frequencies of micronucleated RETs (MNRETs) in peripheral blood and of micronucleated polychromatic erythrocytes (MNPCEs) in bone marrow were comparable, as shown in two laboratories independently. The maximum frequencies of MNRETs in peripheral blood lagged about 24 and 12 h behind those of MNPCEs in bone marrow in experiments with 24- and 12-h sampling intervals, respectively. The proportion of each type of RET was examined periodically after treatment with ara-C at doses ranging from 6.25 to 50.0 mg/kg. The proportion of type I RETs among total RETs decreased 24 or 48 h after treatment according to the dose level. This suggest that this ratio could be a good indicator of the bone marrow cell toxicity of test chemicals.

Authors+Show Affiliations

Iwakura K
Research Laboratories, Nippon Shinyaku Co., Ltd., Kyoto, Japan.
Tamura H
No affiliation info available
Matsumoto A
No affiliation info available
Ajimi S
No affiliation info available
Ogura S
No affiliation info available
Kakimoto K
No affiliation info available
Matsumoto T
No affiliation info available
Hayashi M
No affiliation info available

MeSH

Acridine OrangeAnimalsCytarabineDose-Response Relationship, DrugLaboratoriesMaleMiceMice, Inbred StrainsMicronucleus TestsMitomycinMutagensReticulocytes

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

1372695

Citation

Iwakura, K, et al. "The Micronucleus Assay With Peripheral Blood Reticulocytes By Acridine Orange Supravital Staining With 1-beta-D-arabinofuranosylcytosine." Mutation Research, vol. 278, no. 2-3, 1992, pp. 131-7.
Iwakura K, Tamura H, Matsumoto A, et al. The micronucleus assay with peripheral blood reticulocytes by acridine orange supravital staining with 1-beta-D-arabinofuranosylcytosine. Mutat Res. 1992;278(2-3):131-7.
Iwakura, K., Tamura, H., Matsumoto, A., Ajimi, S., Ogura, S., Kakimoto, K., Matsumoto, T., & Hayashi, M. (1992). The micronucleus assay with peripheral blood reticulocytes by acridine orange supravital staining with 1-beta-D-arabinofuranosylcytosine. Mutation Research, 278(2-3), 131-7.
Iwakura K, et al. The Micronucleus Assay With Peripheral Blood Reticulocytes By Acridine Orange Supravital Staining With 1-beta-D-arabinofuranosylcytosine. Mutat Res. 1992 Feb-Mar;278(2-3):131-7. PubMed PMID: 1372695.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The micronucleus assay with peripheral blood reticulocytes by acridine orange supravital staining with 1-beta-D-arabinofuranosylcytosine. AU - Iwakura,K, AU - Tamura,H, AU - Matsumoto,A, AU - Ajimi,S, AU - Ogura,S, AU - Kakimoto,K, AU - Matsumoto,T, AU - Hayashi,M, PY - 1992/2/1/pubmed PY - 1992/2/1/medline PY - 1992/2/1/entrez SP - 131 EP - 7 JF - Mutation research JO - Mutat Res VL - 278 IS - 2-3 N2 - Dose-dependent induction of micronuclei with 1-beta-D-arabinofuranosylcytosine (ara-C) was clearly shown in CD-1 mouse peripheral blood reticulocytes (RETs) using an acridine orange (AO) supravital staining method, as well as in the conventional bone marrow assay. The maximum frequencies of micronucleated RETs (MNRETs) in peripheral blood and of micronucleated polychromatic erythrocytes (MNPCEs) in bone marrow were comparable, as shown in two laboratories independently. The maximum frequencies of MNRETs in peripheral blood lagged about 24 and 12 h behind those of MNPCEs in bone marrow in experiments with 24- and 12-h sampling intervals, respectively. The proportion of each type of RET was examined periodically after treatment with ara-C at doses ranging from 6.25 to 50.0 mg/kg. The proportion of type I RETs among total RETs decreased 24 or 48 h after treatment according to the dose level. This suggest that this ratio could be a good indicator of the bone marrow cell toxicity of test chemicals. SN - 0027-5107 UR - https://www.unboundmedicine.com/medline/citation/1372695/The_micronucleus_assay_with_peripheral_blood_reticulocytes_by_acridine_orange_supravital_staining_with_1_beta_D_arabinofuranosylcytosine_ DB - PRIME DP - Unbound Medicine ER -