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The mouse peripheral blood micronucleus test with 2-acetylaminofluorene using the acridine orange supravital staining method.
Mutat Res. 1992 Feb-Mar; 278(2-3):153-7.MR

Abstract

The peripheral blood micronucleus test using the acridine orange (AO) supravital staining method was validated with the potent bone marrow clastogen 2-acetylaminofluorene (2-AAF). 2-AAF induced micronuclei in peripheral blood reticulocytes dose-dependently as well as in bone marrow polychromatic erythrocytes. The incidence of micronucleated reticulocytes (MNRETs) peaked 48 h after a single treatment in both CD-1 and BDF1 mice, and the incidence of micronucleated polychromatic erythrocytes (MNPCEs) peaked 24 or 48 h after treatment. The maximum incidences of MNRETs were always higher than those of MNPCEs in both mouse strains treated once. In the double-treatment regime, the maximum incidence of MNRETs was observed at 24 h after the second treatment in each strain. The incidences of MNRETs in BDF1 mice were higher than in CD-1 mice after a single treatment but were comparable after double treatment. These results indicate that the peripheral blood micronucleus test using AO supravital staining is as sensitive as the conventional bone marrow assay. The new staining method can be performed more easily than the original smear method using either bone marrow or peripheral blood cells. Thus, the peripheral blood method using AO supravital staining is a possible alternative to the conventional bone marrow assay.

Authors+Show Affiliations

Asano N
Toxicological Research Division, Nitto Denko Corporation, Osaka, Japan.
Hagiwara T
No affiliation info available

MeSH

2-AcetylaminofluoreneAcridine OrangeAnimalsBone MarrowDose-Response Relationship, DrugErythrocytesMaleMiceMice, Inbred StrainsMicronucleus TestsMutagensReticulocytesSpecies Specificity

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

1372698

Citation

Asano, N, and T Hagiwara. "The Mouse Peripheral Blood Micronucleus Test With 2-acetylaminofluorene Using the Acridine Orange Supravital Staining Method." Mutation Research, vol. 278, no. 2-3, 1992, pp. 153-7.
Asano N, Hagiwara T. The mouse peripheral blood micronucleus test with 2-acetylaminofluorene using the acridine orange supravital staining method. Mutat Res. 1992;278(2-3):153-7.
Asano, N., & Hagiwara, T. (1992). The mouse peripheral blood micronucleus test with 2-acetylaminofluorene using the acridine orange supravital staining method. Mutation Research, 278(2-3), 153-7.
Asano N, Hagiwara T. The Mouse Peripheral Blood Micronucleus Test With 2-acetylaminofluorene Using the Acridine Orange Supravital Staining Method. Mutat Res. 1992 Feb-Mar;278(2-3):153-7. PubMed PMID: 1372698.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The mouse peripheral blood micronucleus test with 2-acetylaminofluorene using the acridine orange supravital staining method. AU - Asano,N, AU - Hagiwara,T, PY - 1992/2/1/pubmed PY - 1992/2/1/medline PY - 1992/2/1/entrez SP - 153 EP - 7 JF - Mutation research JO - Mutat Res VL - 278 IS - 2-3 N2 - The peripheral blood micronucleus test using the acridine orange (AO) supravital staining method was validated with the potent bone marrow clastogen 2-acetylaminofluorene (2-AAF). 2-AAF induced micronuclei in peripheral blood reticulocytes dose-dependently as well as in bone marrow polychromatic erythrocytes. The incidence of micronucleated reticulocytes (MNRETs) peaked 48 h after a single treatment in both CD-1 and BDF1 mice, and the incidence of micronucleated polychromatic erythrocytes (MNPCEs) peaked 24 or 48 h after treatment. The maximum incidences of MNRETs were always higher than those of MNPCEs in both mouse strains treated once. In the double-treatment regime, the maximum incidence of MNRETs was observed at 24 h after the second treatment in each strain. The incidences of MNRETs in BDF1 mice were higher than in CD-1 mice after a single treatment but were comparable after double treatment. These results indicate that the peripheral blood micronucleus test using AO supravital staining is as sensitive as the conventional bone marrow assay. The new staining method can be performed more easily than the original smear method using either bone marrow or peripheral blood cells. Thus, the peripheral blood method using AO supravital staining is a possible alternative to the conventional bone marrow assay. SN - 0027-5107 UR - https://www.unboundmedicine.com/medline/citation/1372698/The_mouse_peripheral_blood_micronucleus_test_with_2_acetylaminofluorene_using_the_acridine_orange_supravital_staining_method_ DB - PRIME DP - Unbound Medicine ER -