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Validation of the mouse peripheral blood micronucleus assay using acridine orange supravital staining with urethane.
Mutat Res. 1992 Feb-Mar; 278(2-3):205-8.MR

Abstract

The mouse peripheral blood micronucleus assay using acridine orange supravital staining was compared with the standard bone marrow assay using urethane (ethyl carbamate)-treated mice. Urethane was intraperitoneally injected to CD-1 and BDF1 mice at doses ranging from 62 to 1000 and 62 to 250 mg/kg, respectively. Peripheral blood was collected from the tail 0, 24, 48, and 72 h and bone marrow cells were smeared at 24 and 42 h after the treatment. Although the response of micronucleus induction in peripheral reticulocytes was delayed by about 24 h compared to that in bone marrow polychromatic erythrocytes, the maximum frequencies of micronucleated young erythrocytes were comparable. Therefore, the peripheral blood micronucleus assay using the acridine orange supravital staining method may provide a good alternative to the conventional bone marrow assay.

Authors+Show Affiliations

Kishi M
Section of Pharmacology and Toxicology, Kanagawa Prefectural Public Health Laboratories, Yokohama, Japan.
Horiguchi Y
No affiliation info available
Watanabe S
No affiliation info available
Hayashi M
No affiliation info available

MeSH

Acridine OrangeAnimalsBone MarrowDose-Response Relationship, DrugMaleMiceMice, Inbred StrainsMicronucleus TestsMutagensReticulocytesSpecies SpecificityUrethane

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

1372707

Citation

Kishi, M, et al. "Validation of the Mouse Peripheral Blood Micronucleus Assay Using Acridine Orange Supravital Staining With Urethane." Mutation Research, vol. 278, no. 2-3, 1992, pp. 205-8.
Kishi M, Horiguchi Y, Watanabe S, et al. Validation of the mouse peripheral blood micronucleus assay using acridine orange supravital staining with urethane. Mutat Res. 1992;278(2-3):205-8.
Kishi, M., Horiguchi, Y., Watanabe, S., & Hayashi, M. (1992). Validation of the mouse peripheral blood micronucleus assay using acridine orange supravital staining with urethane. Mutation Research, 278(2-3), 205-8.
Kishi M, et al. Validation of the Mouse Peripheral Blood Micronucleus Assay Using Acridine Orange Supravital Staining With Urethane. Mutat Res. 1992 Feb-Mar;278(2-3):205-8. PubMed PMID: 1372707.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Validation of the mouse peripheral blood micronucleus assay using acridine orange supravital staining with urethane. AU - Kishi,M, AU - Horiguchi,Y, AU - Watanabe,S, AU - Hayashi,M, PY - 1992/2/1/pubmed PY - 1992/2/1/medline PY - 1992/2/1/entrez SP - 205 EP - 8 JF - Mutation research JO - Mutat Res VL - 278 IS - 2-3 N2 - The mouse peripheral blood micronucleus assay using acridine orange supravital staining was compared with the standard bone marrow assay using urethane (ethyl carbamate)-treated mice. Urethane was intraperitoneally injected to CD-1 and BDF1 mice at doses ranging from 62 to 1000 and 62 to 250 mg/kg, respectively. Peripheral blood was collected from the tail 0, 24, 48, and 72 h and bone marrow cells were smeared at 24 and 42 h after the treatment. Although the response of micronucleus induction in peripheral reticulocytes was delayed by about 24 h compared to that in bone marrow polychromatic erythrocytes, the maximum frequencies of micronucleated young erythrocytes were comparable. Therefore, the peripheral blood micronucleus assay using the acridine orange supravital staining method may provide a good alternative to the conventional bone marrow assay. SN - 0027-5107 UR - https://www.unboundmedicine.com/medline/citation/1372707/Validation_of_the_mouse_peripheral_blood_micronucleus_assay_using_acridine_orange_supravital_staining_with_urethane_ DB - PRIME DP - Unbound Medicine ER -