Tags

Type your tag names separated by a space and hit enter

Analysis of direct tissue isoelectric focused protein profiles of resected intestinal mucosa and endoscopic biopsies from patients with inflammatory bowel disease.
Clin Invest Med. 1992 Feb; 15(1):49-59.CI

Abstract

Direct tissue isoelectric focusing was used as a procedure to analyze differences in soluble tissue protein profiles of resected intestinal segments and endoscopic biopsies from patients with ulcerative colitis, Crohn's disease, and colonic cancer. Extraction of tissue proteins was accomplished by electrophoresis of mucosal cryostat sections on agarose gels across a broad pH gradient. The inflamed colonic mucosa from Crohn's disease patients showed similar isoelectric focusing protein patterns. Small bowel mucosa from a patient with both colonic diverticular disease and Crohn's disease showed protein patterns identical with that of the mucosa from a patient with only Crohn's disease. The inflamed mucosae from ulcerative colitis patients revealed identical protein patterns but were distinct from those of non-inflamed ulcerative colitis mucosa and from the inflamed mucosae from Crohn's disease patients. Non-inflamed small bowel mucosae from cancer, ulcerative colitis, and Crohn's disease patients showed distinct protein patterns which were absent in the non-inflamed large bowel mucosae. The inflamed resected ileum of a Crohn's disease patient exhibited protein patterns similar to those of the biopsy of an inflamed mid-transverse large bowel. Mucosal biopsies from inflamed sigmoid colon of a Crohn's disease patient showed different protein patterns than those in biopsies from the inflamed mid-transverse colon. Thus, distinctive isoelectric focusing protein patterns may be useful in differentiating Crohn's colitis and ulcerative colitis when granulomata are absent, and in resolving indeterminant colitis to one of these classic inflammatory bowel diseases.

Authors+Show Affiliations

Gastrointestinal Diseases Research Unit, Hôtel-Dieu Hospital, Kingston, Ontario.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1374000

Citation

Szewczuk, M R., and W T. Depew. "Analysis of Direct Tissue Isoelectric Focused Protein Profiles of Resected Intestinal Mucosa and Endoscopic Biopsies From Patients With Inflammatory Bowel Disease." Clinical and Investigative Medicine. Medecine Clinique Et Experimentale, vol. 15, no. 1, 1992, pp. 49-59.
Szewczuk MR, Depew WT. Analysis of direct tissue isoelectric focused protein profiles of resected intestinal mucosa and endoscopic biopsies from patients with inflammatory bowel disease. Clin Invest Med. 1992;15(1):49-59.
Szewczuk, M. R., & Depew, W. T. (1992). Analysis of direct tissue isoelectric focused protein profiles of resected intestinal mucosa and endoscopic biopsies from patients with inflammatory bowel disease. Clinical and Investigative Medicine. Medecine Clinique Et Experimentale, 15(1), 49-59.
Szewczuk MR, Depew WT. Analysis of Direct Tissue Isoelectric Focused Protein Profiles of Resected Intestinal Mucosa and Endoscopic Biopsies From Patients With Inflammatory Bowel Disease. Clin Invest Med. 1992;15(1):49-59. PubMed PMID: 1374000.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of direct tissue isoelectric focused protein profiles of resected intestinal mucosa and endoscopic biopsies from patients with inflammatory bowel disease. AU - Szewczuk,M R, AU - Depew,W T, PY - 1992/2/1/pubmed PY - 1992/2/1/medline PY - 1992/2/1/entrez SP - 49 EP - 59 JF - Clinical and investigative medicine. Medecine clinique et experimentale JO - Clin Invest Med VL - 15 IS - 1 N2 - Direct tissue isoelectric focusing was used as a procedure to analyze differences in soluble tissue protein profiles of resected intestinal segments and endoscopic biopsies from patients with ulcerative colitis, Crohn's disease, and colonic cancer. Extraction of tissue proteins was accomplished by electrophoresis of mucosal cryostat sections on agarose gels across a broad pH gradient. The inflamed colonic mucosa from Crohn's disease patients showed similar isoelectric focusing protein patterns. Small bowel mucosa from a patient with both colonic diverticular disease and Crohn's disease showed protein patterns identical with that of the mucosa from a patient with only Crohn's disease. The inflamed mucosae from ulcerative colitis patients revealed identical protein patterns but were distinct from those of non-inflamed ulcerative colitis mucosa and from the inflamed mucosae from Crohn's disease patients. Non-inflamed small bowel mucosae from cancer, ulcerative colitis, and Crohn's disease patients showed distinct protein patterns which were absent in the non-inflamed large bowel mucosae. The inflamed resected ileum of a Crohn's disease patient exhibited protein patterns similar to those of the biopsy of an inflamed mid-transverse large bowel. Mucosal biopsies from inflamed sigmoid colon of a Crohn's disease patient showed different protein patterns than those in biopsies from the inflamed mid-transverse colon. Thus, distinctive isoelectric focusing protein patterns may be useful in differentiating Crohn's colitis and ulcerative colitis when granulomata are absent, and in resolving indeterminant colitis to one of these classic inflammatory bowel diseases. SN - 0147-958X UR - https://www.unboundmedicine.com/medline/citation/1374000/Analysis_of_direct_tissue_isoelectric_focused_protein_profiles_of_resected_intestinal_mucosa_and_endoscopic_biopsies_from_patients_with_inflammatory_bowel_disease_ DB - PRIME DP - Unbound Medicine ER -