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Monoclonal antibody to Pneumocystis carinii. Comparison with silver stain in bronchial lavage specimens.
Am J Clin Pathol. 1992 May; 97(5):619-24.AJ

Abstract

Monoclonal 3F6 anti-Pneumocystis carinii antibody (MAB-3F6) was used to stain cell blocks from 164 bronchial lavage specimens from patients with the acquired immune deficiency syndrome (AIDS) and AIDS-related complex and compared with slides stained with Grocott's modification of the Gomori methenamine silver stain. Pneumocystis organisms were present in 83 of 164 cases using MAB-3F6 stain, whereas Grocott's modified silver stain demonstrated Pneumocystis organisms in 48. MAB-3F6 demonstrated Pneumocystis organisms in 38 cases with negative silver stains, whereas silver stain identified Pneumocystis organisms in only three MAB-3F6-negative cases. Of 70 patients with clinical Pneumocystis pneumonia at the time of the specimen was obtained, 59 had MAB-3F6-positive specimens, whereas 39 had organisms detected using Grocott's modified silver stain. Of 37 patients without clinically apparent Pneumocystis pneumonia any time in their course, 4 had abundant organisms and 33 had negative stains with MAB-3F6. MAB-3F6 detected Pneumocystis organisms in 22 of 31 cases of Pneumocystis pneumonia that had no organisms identified using Grocott's silver stain (X2 = 5.76, P = 0.016). MAB-3F6 immunochemical staining is a more sensitive method than Grocott's modified silver stain to detect Pneumocystis organisms.

Authors+Show Affiliations

Department of Pathology, Parkland Memorial Hospital, University of Texas Southwestern Medical Center, Dallas 75235-9072.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1374213

Citation

Homer, K S., et al. "Monoclonal Antibody to Pneumocystis Carinii. Comparison With Silver Stain in Bronchial Lavage Specimens." American Journal of Clinical Pathology, vol. 97, no. 5, 1992, pp. 619-24.
Homer KS, Wiley EL, Smith AL, et al. Monoclonal antibody to Pneumocystis carinii. Comparison with silver stain in bronchial lavage specimens. Am J Clin Pathol. 1992;97(5):619-24.
Homer, K. S., Wiley, E. L., Smith, A. L., McCollough, L., Clark, D., Nightingale, S. D., & Vuitch, F. (1992). Monoclonal antibody to Pneumocystis carinii. Comparison with silver stain in bronchial lavage specimens. American Journal of Clinical Pathology, 97(5), 619-24.
Homer KS, et al. Monoclonal Antibody to Pneumocystis Carinii. Comparison With Silver Stain in Bronchial Lavage Specimens. Am J Clin Pathol. 1992;97(5):619-24. PubMed PMID: 1374213.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Monoclonal antibody to Pneumocystis carinii. Comparison with silver stain in bronchial lavage specimens. AU - Homer,K S, AU - Wiley,E L, AU - Smith,A L, AU - McCollough,L, AU - Clark,D, AU - Nightingale,S D, AU - Vuitch,F, PY - 1992/5/1/pubmed PY - 1992/5/1/medline PY - 1992/5/1/entrez SP - 619 EP - 24 JF - American journal of clinical pathology JO - Am J Clin Pathol VL - 97 IS - 5 N2 - Monoclonal 3F6 anti-Pneumocystis carinii antibody (MAB-3F6) was used to stain cell blocks from 164 bronchial lavage specimens from patients with the acquired immune deficiency syndrome (AIDS) and AIDS-related complex and compared with slides stained with Grocott's modification of the Gomori methenamine silver stain. Pneumocystis organisms were present in 83 of 164 cases using MAB-3F6 stain, whereas Grocott's modified silver stain demonstrated Pneumocystis organisms in 48. MAB-3F6 demonstrated Pneumocystis organisms in 38 cases with negative silver stains, whereas silver stain identified Pneumocystis organisms in only three MAB-3F6-negative cases. Of 70 patients with clinical Pneumocystis pneumonia at the time of the specimen was obtained, 59 had MAB-3F6-positive specimens, whereas 39 had organisms detected using Grocott's modified silver stain. Of 37 patients without clinically apparent Pneumocystis pneumonia any time in their course, 4 had abundant organisms and 33 had negative stains with MAB-3F6. MAB-3F6 detected Pneumocystis organisms in 22 of 31 cases of Pneumocystis pneumonia that had no organisms identified using Grocott's silver stain (X2 = 5.76, P = 0.016). MAB-3F6 immunochemical staining is a more sensitive method than Grocott's modified silver stain to detect Pneumocystis organisms. SN - 0002-9173 UR - https://www.unboundmedicine.com/medline/citation/1374213/Monoclonal_antibody_to_Pneumocystis_carinii__Comparison_with_silver_stain_in_bronchial_lavage_specimens_ L2 - https://academic.oup.com/ajcp/article-lookup/doi/10.1093/ajcp/97.5.619 DB - PRIME DP - Unbound Medicine ER -