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One-carbon metabolism in Neurospora crassa wild-type and in mutants partially deficient in serine hydroxymethyltransferase.
Biochem J. 1976 Nov 15; 160(2):305-14.BJ

Abstract

1. The concentrations of folate-dependent enzymes in Neurospora crassa Lindegren A wild type (FGSC no. 853), Ser-l mutant, strain H605a (FGSC no. 118), and for mutant, strain C-24 (FGSC no. 9), were compared during exponential growth on defined minimal media. Both mutants were partially lacking in serine hydroxymethyltransferase, but contained higher concentrations of 10-formyltetrahydrofolate synthetase than did the wild type. Mycelia of the mutants contained higher concentrations of these enzymes when growth media were supplemented with 1mM-glycine. In the wild-type, this glycine supplement also increased the specific activities of 5,10-methylenetetrahydrofolate dehydrogenase and 5,10-methylenetetrahydrofolate reductase. 5. During growth, total folate and polyglutamyl folate concentrations were greatest in the wild-type. Methylfolates were not detected in mutant Ser-l, and were only present in the for mutant after growth in glycine-supplemented media. Exogenous glycine increased folate concentration threefold in the wild type, mainly owing to increases in unsubstituted polyglutamyl derivatives. 3. Feeding experiments using 14C-labelled substrates showed that C1 units were generated from formate, glycine and serine in the wild type. Greater incorporation of 14C occurred when mycelia were cultured in glycine-supplemented media. Formate and serine were precursors of C1 units in the mutants, but the ability to cleave glycine was slight or lacking.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

137722

Citation

Cossins, E A., et al. "One-carbon Metabolism in Neurospora Crassa Wild-type and in Mutants Partially Deficient in Serine Hydroxymethyltransferase." The Biochemical Journal, vol. 160, no. 2, 1976, pp. 305-14.
Cossins EA, Chan PY, Combepine G. One-carbon metabolism in Neurospora crassa wild-type and in mutants partially deficient in serine hydroxymethyltransferase. Biochem J. 1976;160(2):305-14.
Cossins, E. A., Chan, P. Y., & Combepine, G. (1976). One-carbon metabolism in Neurospora crassa wild-type and in mutants partially deficient in serine hydroxymethyltransferase. The Biochemical Journal, 160(2), 305-14.
Cossins EA, Chan PY, Combepine G. One-carbon Metabolism in Neurospora Crassa Wild-type and in Mutants Partially Deficient in Serine Hydroxymethyltransferase. Biochem J. 1976 Nov 15;160(2):305-14. PubMed PMID: 137722.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - One-carbon metabolism in Neurospora crassa wild-type and in mutants partially deficient in serine hydroxymethyltransferase. AU - Cossins,E A, AU - Chan,P Y, AU - Combepine,G, PY - 1976/11/15/pubmed PY - 1976/11/15/medline PY - 1976/11/15/entrez SP - 305 EP - 14 JF - The Biochemical journal JO - Biochem. J. VL - 160 IS - 2 N2 - 1. The concentrations of folate-dependent enzymes in Neurospora crassa Lindegren A wild type (FGSC no. 853), Ser-l mutant, strain H605a (FGSC no. 118), and for mutant, strain C-24 (FGSC no. 9), were compared during exponential growth on defined minimal media. Both mutants were partially lacking in serine hydroxymethyltransferase, but contained higher concentrations of 10-formyltetrahydrofolate synthetase than did the wild type. Mycelia of the mutants contained higher concentrations of these enzymes when growth media were supplemented with 1mM-glycine. In the wild-type, this glycine supplement also increased the specific activities of 5,10-methylenetetrahydrofolate dehydrogenase and 5,10-methylenetetrahydrofolate reductase. 5. During growth, total folate and polyglutamyl folate concentrations were greatest in the wild-type. Methylfolates were not detected in mutant Ser-l, and were only present in the for mutant after growth in glycine-supplemented media. Exogenous glycine increased folate concentration threefold in the wild type, mainly owing to increases in unsubstituted polyglutamyl derivatives. 3. Feeding experiments using 14C-labelled substrates showed that C1 units were generated from formate, glycine and serine in the wild type. Greater incorporation of 14C occurred when mycelia were cultured in glycine-supplemented media. Formate and serine were precursors of C1 units in the mutants, but the ability to cleave glycine was slight or lacking. SN - 0264-6021 UR - https://www.unboundmedicine.com/medline/citation/137722/One_carbon_metabolism_in_Neurospora_crassa_wild_type_and_in_mutants_partially_deficient_in_serine_hydroxymethyltransferase_ L2 - https://portlandpress.com/biochemj/article-lookup/doi/10.1042/bj1600305 DB - PRIME DP - Unbound Medicine ER -