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Coagulation dynamics after hemodilution with polyhemoglobin.
Surg Gynecol Obstet. 1992 Sep; 175(3):219-26.SG

Abstract

The effect of polyhemoglobin solution (PHS), a candidate erythrocyte cell substitute, on blood coagulation, was investigated. Whole blood samples from six male Sprague-Dawley rats were diluted in vitro 3:1, 1:1 and 1:3 with normal saline solution, stroma-free hemoglobin (SFH) (7 grams of hemoglobin per deciliter), PHS (14 grams of hemoglobin per deciliter) or 5 percent bovine albumin (ALB) and tested with a thrombelastograph (TEG) for effects of hemodilution on coagulation. For in vivo tests, 24 rats were randomly divided into four groups of six each and 50 percent of the estimated blood volume was replaced with SFH, PHS, ALB or 6 percent hydroxyethyl starch. Blood samples collected before and after the hemodilution were tested with a TEG. The TEG parameters (r, k, Ma, and fi) were determined and analyzed statistically. At 50 percent in vitro or in vivo hemodilution, PHS did not cause significant alteration (p greater than 0.05) in initial coagulation mechanism, while SFH slightly, but significantly (p less than 0.05), accelerated coagulation. Tensile strength of formed clot (Ma) did decrease after hemodilution with PHS (p less than 0.05), but the effect was attributable to dilutional effect. At moderate hemodilution (50 percent), PHS does not seem to cause undue coagulopathy in this rat model.

Authors+Show Affiliations

Department of Surgery, Miriam Hospital, Providence, Rhode Island 02906.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1381112

Citation

Kim, H W., et al. "Coagulation Dynamics After Hemodilution With Polyhemoglobin." Surgery, Gynecology & Obstetrics, vol. 175, no. 3, 1992, pp. 219-26.
Kim HW, Stubdal H, Greenburg AG. Coagulation dynamics after hemodilution with polyhemoglobin. Surg Gynecol Obstet. 1992;175(3):219-26.
Kim, H. W., Stubdal, H., & Greenburg, A. G. (1992). Coagulation dynamics after hemodilution with polyhemoglobin. Surgery, Gynecology & Obstetrics, 175(3), 219-26.
Kim HW, Stubdal H, Greenburg AG. Coagulation Dynamics After Hemodilution With Polyhemoglobin. Surg Gynecol Obstet. 1992;175(3):219-26. PubMed PMID: 1381112.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Coagulation dynamics after hemodilution with polyhemoglobin. AU - Kim,H W, AU - Stubdal,H, AU - Greenburg,A G, PY - 1992/9/1/pubmed PY - 1992/9/1/medline PY - 1992/9/1/entrez SP - 219 EP - 26 JF - Surgery, gynecology & obstetrics JO - Surg Gynecol Obstet VL - 175 IS - 3 N2 - The effect of polyhemoglobin solution (PHS), a candidate erythrocyte cell substitute, on blood coagulation, was investigated. Whole blood samples from six male Sprague-Dawley rats were diluted in vitro 3:1, 1:1 and 1:3 with normal saline solution, stroma-free hemoglobin (SFH) (7 grams of hemoglobin per deciliter), PHS (14 grams of hemoglobin per deciliter) or 5 percent bovine albumin (ALB) and tested with a thrombelastograph (TEG) for effects of hemodilution on coagulation. For in vivo tests, 24 rats were randomly divided into four groups of six each and 50 percent of the estimated blood volume was replaced with SFH, PHS, ALB or 6 percent hydroxyethyl starch. Blood samples collected before and after the hemodilution were tested with a TEG. The TEG parameters (r, k, Ma, and fi) were determined and analyzed statistically. At 50 percent in vitro or in vivo hemodilution, PHS did not cause significant alteration (p greater than 0.05) in initial coagulation mechanism, while SFH slightly, but significantly (p less than 0.05), accelerated coagulation. Tensile strength of formed clot (Ma) did decrease after hemodilution with PHS (p less than 0.05), but the effect was attributable to dilutional effect. At moderate hemodilution (50 percent), PHS does not seem to cause undue coagulopathy in this rat model. SN - 0039-6087 UR - https://www.unboundmedicine.com/medline/citation/1381112/Coagulation_dynamics_after_hemodilution_with_polyhemoglobin_ DB - PRIME DP - Unbound Medicine ER -