Tags

Type your tag names separated by a space and hit enter

Antibody responses to the 18-kDa protein of Mycobacterium leprae in leprosy and tuberculosis patients.
Int J Lepr Other Mycobact Dis. 1992 Jun; 60(2):201-7.IJ

Abstract

The 18-kDa protein of Mycobacterium leprae, as recognized by the monoclonal antibody L5, has a restricted species distribution, being confined to M. leprae and M. habana. We have developed a solid-phase ELISA using purified, recombinant M. leprae 18-kDa protein and compared the serological responses of Nepali leprosy and tuberculosis patients and endemic control subjects to the protein and the M. leprae phenolic glycolipid-I (PGL-I). Few control subjects had anti-18-kDa antibodies. A small proportion of paucibacillary (PB) leprosy and 42% of multibacillary (MB) leprosy patients had IgG anti-M. leprae antibodies. A similar proportion (47%) of Nepali tuberculosis (TB) patients were seropositive, and IgG anti-18-kDa antibody levels were significantly higher in MB and TB patients than in control subjects. By comparison, IgM anti-PGL-I antibodies were detected in 88% of MB leprosy patients and only 7% of TB patients. The possible reasons for the 18-kDa protein seroreactivity in TB patients are discussed, and the anti-18-kDa assay is compared with other antibody assays for protein and nonprotein antigens of M. leprae. It is concluded that the sensitivity and specificity of the anti-M. leprae 18-kDa ELISA are insufficient for the assay to be of clinical utility in leprosy patients.

Authors+Show Affiliations

Roche PW
Mycobacterial Research Laboratory, Anandaban Leprosy Hospital, Kathmandu, Nepal.
Prestidge RL
No affiliation info available
Watson JD
No affiliation info available
Britton WJ
No affiliation info available

MeSH

AdolescentAdultAgedAged, 80 and overAntibodies, BacterialAntibodies, MonoclonalAntigens, BacterialBacterial ProteinsChildEpitopesFemaleHumansImmunoglobulin GImmunoglobulin MLeprosyMaleMiddle AgedMycobacterium lepraeRecombinant ProteinsTuberculosis, Pulmonary

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1381739

Citation

Roche, P W., et al. "Antibody Responses to the 18-kDa Protein of Mycobacterium Leprae in Leprosy and Tuberculosis Patients." International Journal of Leprosy and Other Mycobacterial Diseases : Official Organ of the International Leprosy Association, vol. 60, no. 2, 1992, pp. 201-7.
Roche PW, Prestidge RL, Watson JD, et al. Antibody responses to the 18-kDa protein of Mycobacterium leprae in leprosy and tuberculosis patients. Int J Lepr Other Mycobact Dis. 1992;60(2):201-7.
Roche, P. W., Prestidge, R. L., Watson, J. D., & Britton, W. J. (1992). Antibody responses to the 18-kDa protein of Mycobacterium leprae in leprosy and tuberculosis patients. International Journal of Leprosy and Other Mycobacterial Diseases : Official Organ of the International Leprosy Association, 60(2), 201-7.
Roche PW, et al. Antibody Responses to the 18-kDa Protein of Mycobacterium Leprae in Leprosy and Tuberculosis Patients. Int J Lepr Other Mycobact Dis. 1992;60(2):201-7. PubMed PMID: 1381739.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibody responses to the 18-kDa protein of Mycobacterium leprae in leprosy and tuberculosis patients. AU - Roche,P W, AU - Prestidge,R L, AU - Watson,J D, AU - Britton,W J, PY - 1992/6/1/pubmed PY - 1992/6/1/medline PY - 1992/6/1/entrez SP - 201 EP - 7 JF - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association JO - Int J Lepr Other Mycobact Dis VL - 60 IS - 2 N2 - The 18-kDa protein of Mycobacterium leprae, as recognized by the monoclonal antibody L5, has a restricted species distribution, being confined to M. leprae and M. habana. We have developed a solid-phase ELISA using purified, recombinant M. leprae 18-kDa protein and compared the serological responses of Nepali leprosy and tuberculosis patients and endemic control subjects to the protein and the M. leprae phenolic glycolipid-I (PGL-I). Few control subjects had anti-18-kDa antibodies. A small proportion of paucibacillary (PB) leprosy and 42% of multibacillary (MB) leprosy patients had IgG anti-M. leprae antibodies. A similar proportion (47%) of Nepali tuberculosis (TB) patients were seropositive, and IgG anti-18-kDa antibody levels were significantly higher in MB and TB patients than in control subjects. By comparison, IgM anti-PGL-I antibodies were detected in 88% of MB leprosy patients and only 7% of TB patients. The possible reasons for the 18-kDa protein seroreactivity in TB patients are discussed, and the anti-18-kDa assay is compared with other antibody assays for protein and nonprotein antigens of M. leprae. It is concluded that the sensitivity and specificity of the anti-M. leprae 18-kDa ELISA are insufficient for the assay to be of clinical utility in leprosy patients. SN - 0148-916X UR - https://www.unboundmedicine.com/medline/citation/1381739/Antibody_responses_to_the_18_kDa_protein_of_Mycobacterium_leprae_in_leprosy_and_tuberculosis_patients_ DB - PRIME DP - Unbound Medicine ER -