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Regional amines levels in the rat brain following intra-accumbens cholecystokinin and intraperitoneal amphetamine pre-treatment.
Arch Int Physiol Biochim Biophys 1992 May-Jun; 100(3):267-73AI

Abstract

Cholecystokinin octapeptide (CCK8) coexists with dopamine (DA) in a subpopulation of mesolimbic DA neurons including the projections to the nucleus accumbens. In this structure, CCK8 has been reported to exert agonist-like or antagonist-like effects on DA-mediated behaviours and on amphetamine's locomotor-activating effects in rodents. These findings raise the possibility that CCK8 plays a role in modulating the neurochemical mechanisms underlying the effects of DA and amphetamine in the mesolimbic DA system. The purpose of this study was to determine regional tissue monoamine levels in the rat brain and their modulation following injection of CCK8 in the nucleus accumbens. The same paradigm was used to determine the effects of this octapeptide on changes in amine levels induced by amphetamine administered intraperitoneally. DA, norepinephrine (NE), serotonin (5-HT) and their primary metabolites, dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) were measured using a reversed-phase ion pair high-pressure liquid chromatography system with electrochemical detection (HPLC-ED). Frontal cortex displayed the highest DOPAC/DA ratio and the lowest DOPAC/HVA ratio in contrast to the olfactory tubercle. The intraperitoneal injection of amphetamine (1 mg/Kg) followed by the intra-accumbens administration of 0.15M saline decreased the levels of DOPAC and increased DA, 5-HT and 5-HIAA both in nucleus caudatus and nucleus accumbens. The DA agonist induced a decrease in the level of NE in olfactory tubercle and frontal cortex. The direct administration of CCK8 (300 pmol) into the nucleus accumbens decreased the level of DA, DOPAC and 5-HT mainly in olfactory tubercle and nucleus accumbens itself.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Laboratoire de Psychobiologie, Université de Louvain, Louvain-La-Neuve, Belgium.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1382678

Citation

Heidbreder, C, et al. "Regional Amines Levels in the Rat Brain Following Intra-accumbens Cholecystokinin and Intraperitoneal Amphetamine Pre-treatment." Archives Internationales De Physiologie, De Biochimie Et De Biophysique, vol. 100, no. 3, 1992, pp. 267-73.
Heidbreder C, Gewiss M, De Potter W, et al. Regional amines levels in the rat brain following intra-accumbens cholecystokinin and intraperitoneal amphetamine pre-treatment. Arch Int Physiol Biochim Biophys. 1992;100(3):267-73.
Heidbreder, C., Gewiss, M., De Potter, W., & De Witte, P. (1992). Regional amines levels in the rat brain following intra-accumbens cholecystokinin and intraperitoneal amphetamine pre-treatment. Archives Internationales De Physiologie, De Biochimie Et De Biophysique, 100(3), pp. 267-73.
Heidbreder C, et al. Regional Amines Levels in the Rat Brain Following Intra-accumbens Cholecystokinin and Intraperitoneal Amphetamine Pre-treatment. Arch Int Physiol Biochim Biophys. 1992;100(3):267-73. PubMed PMID: 1382678.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regional amines levels in the rat brain following intra-accumbens cholecystokinin and intraperitoneal amphetamine pre-treatment. AU - Heidbreder,C, AU - Gewiss,M, AU - De Potter,W, AU - De Witte,P, PY - 1992/5/1/pubmed PY - 1992/5/1/medline PY - 1992/5/1/entrez SP - 267 EP - 73 JF - Archives internationales de physiologie, de biochimie et de biophysique JO - Arch Int Physiol Biochim Biophys VL - 100 IS - 3 N2 - Cholecystokinin octapeptide (CCK8) coexists with dopamine (DA) in a subpopulation of mesolimbic DA neurons including the projections to the nucleus accumbens. In this structure, CCK8 has been reported to exert agonist-like or antagonist-like effects on DA-mediated behaviours and on amphetamine's locomotor-activating effects in rodents. These findings raise the possibility that CCK8 plays a role in modulating the neurochemical mechanisms underlying the effects of DA and amphetamine in the mesolimbic DA system. The purpose of this study was to determine regional tissue monoamine levels in the rat brain and their modulation following injection of CCK8 in the nucleus accumbens. The same paradigm was used to determine the effects of this octapeptide on changes in amine levels induced by amphetamine administered intraperitoneally. DA, norepinephrine (NE), serotonin (5-HT) and their primary metabolites, dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) were measured using a reversed-phase ion pair high-pressure liquid chromatography system with electrochemical detection (HPLC-ED). Frontal cortex displayed the highest DOPAC/DA ratio and the lowest DOPAC/HVA ratio in contrast to the olfactory tubercle. The intraperitoneal injection of amphetamine (1 mg/Kg) followed by the intra-accumbens administration of 0.15M saline decreased the levels of DOPAC and increased DA, 5-HT and 5-HIAA both in nucleus caudatus and nucleus accumbens. The DA agonist induced a decrease in the level of NE in olfactory tubercle and frontal cortex. The direct administration of CCK8 (300 pmol) into the nucleus accumbens decreased the level of DA, DOPAC and 5-HT mainly in olfactory tubercle and nucleus accumbens itself.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0778-3124 UR - https://www.unboundmedicine.com/medline/citation/1382678/Regional_amines_levels_in_the_rat_brain_following_intra_accumbens_cholecystokinin_and_intraperitoneal_amphetamine_pre_treatment_ DB - PRIME DP - Unbound Medicine ER -