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A ligand-epitope in vitro analysis of major histocompatibility determinants expressed on B and T lymphocytes.
Immunology. 1992 Sep; 77(1):116-22.I

Abstract

Human histocompatibility leucocyte antigen (HLA)-specific monoclonal antibody probes were used to determine the affinity constant, and cell-surface density of HLA class I and class II determinants. The measurements were estimated for single-cell units of B-lymphoblastoid cell line (B-LCL) and cloned activated T cells in different functional states. Each HLA subset showed unimodal affinity constant values for the interaction with the corresponding HLA-specific antibodies. Such values ranged between 2.2 x 10(7) M-1 (class I) and 4.0 x 10(7) M-1 (class II) for different histocompatibility epitopes. In both B and T cells there was a rank order of epitope expression, class I being highly expressed (5 x 10(6) epitopes/cell) followed by DR, DQ and DP, (1.1-3.0 x 10(6) epitopes/cell). Suppressive clones carrying functionally defined stimulating determinants previously designated 'DY' carried similar numbers of DR, DQ and DP binding sites to DY- non-suppressive clones, but showed selective increases of class II determinants reactive with broad class II-specific antibodies. The results are discussed in the context of the functional consequences of different patterns of HLA epitope expression in immune responses.

Authors+Show Affiliations

Department of Immunology, London Hospital Medical College, U.K.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1383136

Citation

Fernandez, N, et al. "A Ligand-epitope in Vitro Analysis of Major Histocompatibility Determinants Expressed On B and T Lymphocytes." Immunology, vol. 77, no. 1, 1992, pp. 116-22.
Fernandez N, Kurpisz M, Labeta M, et al. A ligand-epitope in vitro analysis of major histocompatibility determinants expressed on B and T lymphocytes. Immunology. 1992;77(1):116-22.
Fernandez, N., Kurpisz, M., Labeta, M., Sachs, J., & Pawelec, G. (1992). A ligand-epitope in vitro analysis of major histocompatibility determinants expressed on B and T lymphocytes. Immunology, 77(1), 116-22.
Fernandez N, et al. A Ligand-epitope in Vitro Analysis of Major Histocompatibility Determinants Expressed On B and T Lymphocytes. Immunology. 1992;77(1):116-22. PubMed PMID: 1383136.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A ligand-epitope in vitro analysis of major histocompatibility determinants expressed on B and T lymphocytes. AU - Fernandez,N, AU - Kurpisz,M, AU - Labeta,M, AU - Sachs,J, AU - Pawelec,G, PY - 1992/9/1/pubmed PY - 1992/9/1/medline PY - 1992/9/1/entrez SP - 116 EP - 22 JF - Immunology JO - Immunology VL - 77 IS - 1 N2 - Human histocompatibility leucocyte antigen (HLA)-specific monoclonal antibody probes were used to determine the affinity constant, and cell-surface density of HLA class I and class II determinants. The measurements were estimated for single-cell units of B-lymphoblastoid cell line (B-LCL) and cloned activated T cells in different functional states. Each HLA subset showed unimodal affinity constant values for the interaction with the corresponding HLA-specific antibodies. Such values ranged between 2.2 x 10(7) M-1 (class I) and 4.0 x 10(7) M-1 (class II) for different histocompatibility epitopes. In both B and T cells there was a rank order of epitope expression, class I being highly expressed (5 x 10(6) epitopes/cell) followed by DR, DQ and DP, (1.1-3.0 x 10(6) epitopes/cell). Suppressive clones carrying functionally defined stimulating determinants previously designated 'DY' carried similar numbers of DR, DQ and DP binding sites to DY- non-suppressive clones, but showed selective increases of class II determinants reactive with broad class II-specific antibodies. The results are discussed in the context of the functional consequences of different patterns of HLA epitope expression in immune responses. SN - 0019-2805 UR - https://www.unboundmedicine.com/medline/citation/1383136/A_ligand_epitope_in_vitro_analysis_of_major_histocompatibility_determinants_expressed_on_B_and_T_lymphocytes_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/1383136/ DB - PRIME DP - Unbound Medicine ER -