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Interactions of tumor necrosis factor with granulocyte-macrophage colony-stimulating factor and other cytokines in the regulation of dendritic cell growth in vitro from early bipotent CD34+ progenitors in human bone marrow.
J Immunol. 1992 Oct 15; 149(8):2681-8.JI

Abstract

Colonies of CD1a+ HLA-DR+/DQ+ CD4+ cells with the functional and some of the structural attributes of Langerhans cells are observed in human bone marrow cultures in semi-solid media and are assumed to be the progeny of an early progenitor, the dendritic/Langerhans cell CFU (CFU-DL). The cytokine-regulated growth of these cells has been studied using a chemically defined serum-free system to culture both unfractionated and highly enriched bone marrow progenitor cell populations. Although unfractionated cell growth was optimal in serum replete cultures with PHA-stimulated leukocyte-conditioned medium (PHA-LCM) suboptimal proliferation of CFU-DL was observed in serum even in the absence of PHA-LCM. No colonies were observed under serum-free conditions when granulocyte-macrophage CSF (GM-CSF), IL-3, granulocyte CSF (G-CSF), and macrophage CSF (M-CSF) were present at levels optimal for granulocyte colony-forming unit (CFU-G) and macrophage colony-forming unit (CFU-M) growth. Addition of IL-1 alpha to these cytokines stimulated a small number of CFU-DL. However, in the presence of GM-CSF and IL-3, TNF-alpha or TNF-beta (5 U/ml) were both highly effective in promoting growth up to 82% of optimal and CFU-G growth was also enhanced at these concentrations. TNF was only active during the first 3 days of culture and higher concentrations of TNF-alpha but not TNF-beta were inhibitory for both CFU-DL and CFU-G. CD34+ cell-enriched populations were also enriched for both myeloid progenitors (CFU-G + CFU-M) and CFU-DL to 36- and 48-fold, respectively, and single cell cultures of CD34+ cells yielded single colonies containing both CD1a+ dendritic cells and CD1a- macrophages. Thus dendritic/Langerhans progenitors in the bone marrow expresses CD34, have a capacity for both macrophage and dendritic cell differentiation, and depend on hemopoietic growth factors and TNF for their further development in vitro.

Authors+Show Affiliations

Department of Haematology, Northwick Park Hospital and Clinical Research Centre, Harrow, Middlesex, United Kingdom.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1383322

Citation

Reid, C D., et al. "Interactions of Tumor Necrosis Factor With Granulocyte-macrophage Colony-stimulating Factor and Other Cytokines in the Regulation of Dendritic Cell Growth in Vitro From Early Bipotent CD34+ Progenitors in Human Bone Marrow." Journal of Immunology (Baltimore, Md. : 1950), vol. 149, no. 8, 1992, pp. 2681-8.
Reid CD, Stackpoole A, Meager A, et al. Interactions of tumor necrosis factor with granulocyte-macrophage colony-stimulating factor and other cytokines in the regulation of dendritic cell growth in vitro from early bipotent CD34+ progenitors in human bone marrow. J Immunol. 1992;149(8):2681-8.
Reid, C. D., Stackpoole, A., Meager, A., & Tikerpae, J. (1992). Interactions of tumor necrosis factor with granulocyte-macrophage colony-stimulating factor and other cytokines in the regulation of dendritic cell growth in vitro from early bipotent CD34+ progenitors in human bone marrow. Journal of Immunology (Baltimore, Md. : 1950), 149(8), 2681-8.
Reid CD, et al. Interactions of Tumor Necrosis Factor With Granulocyte-macrophage Colony-stimulating Factor and Other Cytokines in the Regulation of Dendritic Cell Growth in Vitro From Early Bipotent CD34+ Progenitors in Human Bone Marrow. J Immunol. 1992 Oct 15;149(8):2681-8. PubMed PMID: 1383322.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interactions of tumor necrosis factor with granulocyte-macrophage colony-stimulating factor and other cytokines in the regulation of dendritic cell growth in vitro from early bipotent CD34+ progenitors in human bone marrow. AU - Reid,C D, AU - Stackpoole,A, AU - Meager,A, AU - Tikerpae,J, PY - 1992/10/15/pubmed PY - 1992/10/15/medline PY - 1992/10/15/entrez SP - 2681 EP - 8 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 149 IS - 8 N2 - Colonies of CD1a+ HLA-DR+/DQ+ CD4+ cells with the functional and some of the structural attributes of Langerhans cells are observed in human bone marrow cultures in semi-solid media and are assumed to be the progeny of an early progenitor, the dendritic/Langerhans cell CFU (CFU-DL). The cytokine-regulated growth of these cells has been studied using a chemically defined serum-free system to culture both unfractionated and highly enriched bone marrow progenitor cell populations. Although unfractionated cell growth was optimal in serum replete cultures with PHA-stimulated leukocyte-conditioned medium (PHA-LCM) suboptimal proliferation of CFU-DL was observed in serum even in the absence of PHA-LCM. No colonies were observed under serum-free conditions when granulocyte-macrophage CSF (GM-CSF), IL-3, granulocyte CSF (G-CSF), and macrophage CSF (M-CSF) were present at levels optimal for granulocyte colony-forming unit (CFU-G) and macrophage colony-forming unit (CFU-M) growth. Addition of IL-1 alpha to these cytokines stimulated a small number of CFU-DL. However, in the presence of GM-CSF and IL-3, TNF-alpha or TNF-beta (5 U/ml) were both highly effective in promoting growth up to 82% of optimal and CFU-G growth was also enhanced at these concentrations. TNF was only active during the first 3 days of culture and higher concentrations of TNF-alpha but not TNF-beta were inhibitory for both CFU-DL and CFU-G. CD34+ cell-enriched populations were also enriched for both myeloid progenitors (CFU-G + CFU-M) and CFU-DL to 36- and 48-fold, respectively, and single cell cultures of CD34+ cells yielded single colonies containing both CD1a+ dendritic cells and CD1a- macrophages. Thus dendritic/Langerhans progenitors in the bone marrow expresses CD34, have a capacity for both macrophage and dendritic cell differentiation, and depend on hemopoietic growth factors and TNF for their further development in vitro. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/1383322/Interactions_of_tumor_necrosis_factor_with_granulocyte_macrophage_colony_stimulating_factor_and_other_cytokines_in_the_regulation_of_dendritic_cell_growth_in_vitro_from_early_bipotent_CD34+_progenitors_in_human_bone_marrow_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=1383322 DB - PRIME DP - Unbound Medicine ER -