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Regulation of IL-2 receptor subunit genes in human monocytes. Differential effects of IL-2 and IFN-gamma.
J Immunol. 1992 Nov 01; 149(9):2961-8.JI

Abstract

We investigated the effects of IFN-gamma and IL-2 on IL-2R alpha and beta mRNA expression in human monocytes. Low basal expression of IL-2R beta mRNA was detected in fresh monocytes. Stimulation of monocytes with IL-2 induced a significant increase of IL-2R beta mRNA, but did not induce IL-2R alpha mRNA. In contrast, stimulation of monocytes with IFN-gamma-induced IL-2R alpha mRNA, but did not modify IL-2R beta mRNA. Five U/ml of IFN-gamma induced IL-2R alpha mRNA and 2.2 nM of IL-2 induced IL-2R beta mRNA, both within 3 h. Nuclear run-on experiments demonstrated that the induction of IL-2R alpha mRNA by IFN-gamma is controlled, at least in part, at the transcriptional level. In contrast, the enhancement of IL-2R beta mRNA by IL-2 is controlled at a posttranscriptional level and is associated with an increase in the half-life of IL-2R beta mRNA. The results of studies on the cytotoxic activity and on the expression of c-fms mRNA of monocytes activated by the combination of IFN-gamma and IL-2 show that pretreatment with IFN-gamma renders monocytes more sensitive to activation by IL-2. These results demonstrate that the IL-2R alpha and IL-2R beta subunits are induced by different lymphokines through distinct mechanisms and that both receptor subunits can influence the response of monocytes to IL-2.

Authors+Show Affiliations

Immunobiology Section, National Cancer Institute, Frederick Cancer Research Development Center, MD 21702-1201.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1383334

Citation

Espinoza-Delgado, I, et al. "Regulation of IL-2 Receptor Subunit Genes in Human Monocytes. Differential Effects of IL-2 and IFN-gamma." Journal of Immunology (Baltimore, Md. : 1950), vol. 149, no. 9, 1992, pp. 2961-8.
Espinoza-Delgado I, Longo DL, Gusella GL, et al. Regulation of IL-2 receptor subunit genes in human monocytes. Differential effects of IL-2 and IFN-gamma. J Immunol. 1992;149(9):2961-8.
Espinoza-Delgado, I., Longo, D. L., Gusella, G. L., & Varesio, L. (1992). Regulation of IL-2 receptor subunit genes in human monocytes. Differential effects of IL-2 and IFN-gamma. Journal of Immunology (Baltimore, Md. : 1950), 149(9), 2961-8.
Espinoza-Delgado I, et al. Regulation of IL-2 Receptor Subunit Genes in Human Monocytes. Differential Effects of IL-2 and IFN-gamma. J Immunol. 1992 Nov 1;149(9):2961-8. PubMed PMID: 1383334.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of IL-2 receptor subunit genes in human monocytes. Differential effects of IL-2 and IFN-gamma. AU - Espinoza-Delgado,I, AU - Longo,D L, AU - Gusella,G L, AU - Varesio,L, PY - 1992/11/1/pubmed PY - 1992/11/1/medline PY - 1992/11/1/entrez SP - 2961 EP - 8 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 149 IS - 9 N2 - We investigated the effects of IFN-gamma and IL-2 on IL-2R alpha and beta mRNA expression in human monocytes. Low basal expression of IL-2R beta mRNA was detected in fresh monocytes. Stimulation of monocytes with IL-2 induced a significant increase of IL-2R beta mRNA, but did not induce IL-2R alpha mRNA. In contrast, stimulation of monocytes with IFN-gamma-induced IL-2R alpha mRNA, but did not modify IL-2R beta mRNA. Five U/ml of IFN-gamma induced IL-2R alpha mRNA and 2.2 nM of IL-2 induced IL-2R beta mRNA, both within 3 h. Nuclear run-on experiments demonstrated that the induction of IL-2R alpha mRNA by IFN-gamma is controlled, at least in part, at the transcriptional level. In contrast, the enhancement of IL-2R beta mRNA by IL-2 is controlled at a posttranscriptional level and is associated with an increase in the half-life of IL-2R beta mRNA. The results of studies on the cytotoxic activity and on the expression of c-fms mRNA of monocytes activated by the combination of IFN-gamma and IL-2 show that pretreatment with IFN-gamma renders monocytes more sensitive to activation by IL-2. These results demonstrate that the IL-2R alpha and IL-2R beta subunits are induced by different lymphokines through distinct mechanisms and that both receptor subunits can influence the response of monocytes to IL-2. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/1383334/Regulation_of_IL_2_receptor_subunit_genes_in_human_monocytes__Differential_effects_of_IL_2_and_IFN_gamma_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=1383334 DB - PRIME DP - Unbound Medicine ER -