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Neuropeptides modulate human eosinophil chemotaxis.
J Immunol. 1992 Nov 15; 149(10):3309-15.JI

Abstract

To investigate the role of neuropeptides in allergic inflammation, we examined the effect of peptides on eosinophil chemotaxis. Eosinophils were purified from the blood of allergic and normal subjects using a discontinuous Percoll density gradients. Chemotaxis was induced by platelet-activating factor (PAF) and leukotriene B4, and was assayed by a modified Boyden's chamber technique. Four neuropeptides were examined in this study: substance P (SP), neurokinin A, calcitonin gene-related peptide (CGRP), and cholecystokinin octapeptide. Peptides alone (10 nM to 10 microM) were not chemotactic for eosinophils. However, when eosinophils were pre-treated with peptides (100 nM) at 37 degrees C for 30 min, chemotactic response to PAF (10 nM) was significantly enhanced (p < 0.01) in allergic subjects; % control by SP, neurokinin A, CGRP and cholecystokinin octapeptide was 269 +/- 42, 243 +/- 32, 227 +/- 21, and 251 +/- 42, respectively (n = 8). Similar results were obtained in leukotriene B4-induced eosinophil chemotaxis. In contrast, no enhancement was observed in normal subjects. Potentiating effect of SP and CGRP on PAF-induced eosinophil chemotaxis in allergic subjects was significantly attenuated by SP antagonist [D-Pro2,D-Trp7,9]-SP and human CGRP (8-37) receptor antagonist, respectively. Neutral endopeptidase inhibitors (phosphoramidon, leupeptin, and bestatin) failed to significantly augment the PAF-induced eosinophil chemotaxis when the cells were pretreated with various peptides and neutral endopeptidase inhibitors. The C-terminal fragment of SP (SP6-11) had an effect similar to that of the intact SP molecule, whereas no potentiating effect by the N-terminal of SP (SP1-9) was observed. These results suggest that neuropeptides may play a significant role in eosinophil infiltration by priming cells in allergic inflammation.

Authors+Show Affiliations

Allergic Disease Center, Creighton University School of Medicine, Omaha, NE 68178.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1385521

Citation

Numao, T, and D K. Agrawal. "Neuropeptides Modulate Human Eosinophil Chemotaxis." Journal of Immunology (Baltimore, Md. : 1950), vol. 149, no. 10, 1992, pp. 3309-15.
Numao T, Agrawal DK. Neuropeptides modulate human eosinophil chemotaxis. J Immunol. 1992;149(10):3309-15.
Numao, T., & Agrawal, D. K. (1992). Neuropeptides modulate human eosinophil chemotaxis. Journal of Immunology (Baltimore, Md. : 1950), 149(10), 3309-15.
Numao T, Agrawal DK. Neuropeptides Modulate Human Eosinophil Chemotaxis. J Immunol. 1992 Nov 15;149(10):3309-15. PubMed PMID: 1385521.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuropeptides modulate human eosinophil chemotaxis. AU - Numao,T, AU - Agrawal,D K, PY - 1992/11/15/pubmed PY - 1992/11/15/medline PY - 1992/11/15/entrez SP - 3309 EP - 15 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 149 IS - 10 N2 - To investigate the role of neuropeptides in allergic inflammation, we examined the effect of peptides on eosinophil chemotaxis. Eosinophils were purified from the blood of allergic and normal subjects using a discontinuous Percoll density gradients. Chemotaxis was induced by platelet-activating factor (PAF) and leukotriene B4, and was assayed by a modified Boyden's chamber technique. Four neuropeptides were examined in this study: substance P (SP), neurokinin A, calcitonin gene-related peptide (CGRP), and cholecystokinin octapeptide. Peptides alone (10 nM to 10 microM) were not chemotactic for eosinophils. However, when eosinophils were pre-treated with peptides (100 nM) at 37 degrees C for 30 min, chemotactic response to PAF (10 nM) was significantly enhanced (p < 0.01) in allergic subjects; % control by SP, neurokinin A, CGRP and cholecystokinin octapeptide was 269 +/- 42, 243 +/- 32, 227 +/- 21, and 251 +/- 42, respectively (n = 8). Similar results were obtained in leukotriene B4-induced eosinophil chemotaxis. In contrast, no enhancement was observed in normal subjects. Potentiating effect of SP and CGRP on PAF-induced eosinophil chemotaxis in allergic subjects was significantly attenuated by SP antagonist [D-Pro2,D-Trp7,9]-SP and human CGRP (8-37) receptor antagonist, respectively. Neutral endopeptidase inhibitors (phosphoramidon, leupeptin, and bestatin) failed to significantly augment the PAF-induced eosinophil chemotaxis when the cells were pretreated with various peptides and neutral endopeptidase inhibitors. The C-terminal fragment of SP (SP6-11) had an effect similar to that of the intact SP molecule, whereas no potentiating effect by the N-terminal of SP (SP1-9) was observed. These results suggest that neuropeptides may play a significant role in eosinophil infiltration by priming cells in allergic inflammation. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/1385521/Neuropeptides_modulate_human_eosinophil_chemotaxis_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&amp;pmid=1385521 DB - PRIME DP - Unbound Medicine ER -