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Cytokine production by mature and immature CD4-CD8- T cells. Alpha beta-T cell receptor+ CD4-CD8- T cells produce IL-4.
J Immunol. 1992 Aug 15; 149(4):1211-5.JI

Abstract

This study follows our previous investigation describing the production of four cytokines (IL-2, IL-4, IFN-gamma, and TNF-alpha) by subsets of thymocytes defined by the expression of CD3, 4, 8, and 25. Here we investigate in greater detail subpopulations of CD4-CD8- double negative (DN) thymocytes. First we divided immature CD25-CD4-CD8-CD3- (CD25- triple negative) (TN) thymocytes into CD44+ and CD44- subsets. The CD44+ population includes very immature precursor T cells and produced high titers of IL-2, TNF-alpha, and IFN-gamma upon activation with calcium ionophore and phorbol ester. In contrast, the CD44- subset of CD25- TN thymocytes did not produce any of the cytokines studied under similar activation conditions. This observation indicates that the latter subset, which differentiates spontaneously in vitro into CD4+CD8+, already resembles CD4+CD8+ thymocytes (which do not produce any of the tested cytokines). We also subdivided the more mature CD3+ DN thymocytes into TCR-alpha beta- and TCR-gamma delta-bearing subsets. These cells produced cytokines upon activation with solid phase anti-CD3 mAb. gamma delta TCR+ DN thymocytes produced IL-2, IFN-gamma and TNF-alpha, whereas alpha beta TCR+ DN thymocytes produced IL-4, IFN-gamma, and TNF-alpha but not IL-2. We then studied alpha beta TCR+ DN T cells isolated from the spleen and found a similar cytokine production profile. Furthermore, splenic alpha beta TCR+ DN cells showed a TCR V beta gene expression profile reminiscent of alpha beta TCR+ DN thymocytes (predominant use of V beta 8.2). These observations suggest that at least some alpha beta TCR+ DN splenocytes are derived from alpha beta TCR+ DN thymocytes and also raises the possibility that these cells may play a role in the development of Th2 responses through their production of IL-4.

Authors+Show Affiliations

DNAX Research Institute, Palo Alto, CA 94304.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1386860

Citation

Zlotnik, A, et al. "Cytokine Production By Mature and Immature CD4-CD8- T Cells. Alpha beta-T Cell Receptor+ CD4-CD8- T Cells Produce IL-4." Journal of Immunology (Baltimore, Md. : 1950), vol. 149, no. 4, 1992, pp. 1211-5.
Zlotnik A, Godfrey DI, Fischer M, et al. Cytokine production by mature and immature CD4-CD8- T cells. Alpha beta-T cell receptor+ CD4-CD8- T cells produce IL-4. J Immunol. 1992;149(4):1211-5.
Zlotnik, A., Godfrey, D. I., Fischer, M., & Suda, T. (1992). Cytokine production by mature and immature CD4-CD8- T cells. Alpha beta-T cell receptor+ CD4-CD8- T cells produce IL-4. Journal of Immunology (Baltimore, Md. : 1950), 149(4), 1211-5.
Zlotnik A, et al. Cytokine Production By Mature and Immature CD4-CD8- T Cells. Alpha beta-T Cell Receptor+ CD4-CD8- T Cells Produce IL-4. J Immunol. 1992 Aug 15;149(4):1211-5. PubMed PMID: 1386860.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytokine production by mature and immature CD4-CD8- T cells. Alpha beta-T cell receptor+ CD4-CD8- T cells produce IL-4. AU - Zlotnik,A, AU - Godfrey,D I, AU - Fischer,M, AU - Suda,T, PY - 1992/8/15/pubmed PY - 1992/8/15/medline PY - 1992/8/15/entrez SP - 1211 EP - 5 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 149 IS - 4 N2 - This study follows our previous investigation describing the production of four cytokines (IL-2, IL-4, IFN-gamma, and TNF-alpha) by subsets of thymocytes defined by the expression of CD3, 4, 8, and 25. Here we investigate in greater detail subpopulations of CD4-CD8- double negative (DN) thymocytes. First we divided immature CD25-CD4-CD8-CD3- (CD25- triple negative) (TN) thymocytes into CD44+ and CD44- subsets. The CD44+ population includes very immature precursor T cells and produced high titers of IL-2, TNF-alpha, and IFN-gamma upon activation with calcium ionophore and phorbol ester. In contrast, the CD44- subset of CD25- TN thymocytes did not produce any of the cytokines studied under similar activation conditions. This observation indicates that the latter subset, which differentiates spontaneously in vitro into CD4+CD8+, already resembles CD4+CD8+ thymocytes (which do not produce any of the tested cytokines). We also subdivided the more mature CD3+ DN thymocytes into TCR-alpha beta- and TCR-gamma delta-bearing subsets. These cells produced cytokines upon activation with solid phase anti-CD3 mAb. gamma delta TCR+ DN thymocytes produced IL-2, IFN-gamma and TNF-alpha, whereas alpha beta TCR+ DN thymocytes produced IL-4, IFN-gamma, and TNF-alpha but not IL-2. We then studied alpha beta TCR+ DN T cells isolated from the spleen and found a similar cytokine production profile. Furthermore, splenic alpha beta TCR+ DN cells showed a TCR V beta gene expression profile reminiscent of alpha beta TCR+ DN thymocytes (predominant use of V beta 8.2). These observations suggest that at least some alpha beta TCR+ DN splenocytes are derived from alpha beta TCR+ DN thymocytes and also raises the possibility that these cells may play a role in the development of Th2 responses through their production of IL-4. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/1386860/Cytokine_production_by_mature_and_immature_CD4_CD8__T_cells__Alpha_beta_T_cell_receptor+_CD4_CD8__T_cells_produce_IL_4_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=1386860 DB - PRIME DP - Unbound Medicine ER -