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Dual role of IL-7 in the growth and differentiation of immature thymocytes.
Exp Hematol. 1992 Sep; 20(8):998-1003.EH

Abstract

The effects of interleukin 7 (IL-7) on subpopulations of CD4-CD8- thymocytes from young adult mice were tested in vitro. When highly purified CD3-CD4-CD8-thymocytes were cultured in the presence of recombinant IL-7, significant proportions of them became CD4+ and/or CD8+ within a day. CD3+ cells were also detected after 2 days. CD3-CD4-CD8- thymocytes were further subdivided into interleukin 2 receptor (IL-2R)- and IL-2R+ populations. The majority of the IL-2R- cells became CD4+ and/or CD8+ in 1 day in the presence of IL-7, and a substantial proportion of them also became CD3+ in 2-3 days. No significant number of CD4+ or CD8+ cells were generated from the IL-2R+ population under the same conditions. However, a small but significant proportion of them became CD3+ in 3-day cultures with IL-7. Although CD4+/CD8+ cells were also generated from the IL-2R- population in 1-day cultures in the absence of IL-7, the viability of the cells declined rapidly, and no significant numbers of CD3+ cells were generated. In proliferation assays, IL-7 alone vigorously stimulated relatively minor subpopulations of CD4-CD8- thymocytes. The IL-7-responsive cells were CD3+, did not express the IL-2R or the heat-stable antigen M1/69, and included both T-cell receptor (TCR)alpha beta + and TCR alpha beta- populations, the latter most likely TCR gamma delta +. The CD3+CD4-CD8- thymocytes, stimulated with IL-7 for 3 days, remained CD4-CD8-. These results demonstrate important roles of IL-7 in the growth and differentiation of CD4-CD8- thymocytes in vitro. It functions as a survival factor and allows CD3-CD4-CD8- cells to undergo their precommitted differentiation without inducing their proliferation, and it also stimulates CD3+CD4-CD8-thymocytes to proliferate without inducing their differentiation.

Authors+Show Affiliations

Department of Pathology, University of British Colombia, Vancouver, Canada.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1387092

Citation

Vissinga, C S., et al. "Dual Role of IL-7 in the Growth and Differentiation of Immature Thymocytes." Experimental Hematology, vol. 20, no. 8, 1992, pp. 998-1003.
Vissinga CS, Fatur-Saunders DJ, Takei F. Dual role of IL-7 in the growth and differentiation of immature thymocytes. Exp Hematol. 1992;20(8):998-1003.
Vissinga, C. S., Fatur-Saunders, D. J., & Takei, F. (1992). Dual role of IL-7 in the growth and differentiation of immature thymocytes. Experimental Hematology, 20(8), 998-1003.
Vissinga CS, Fatur-Saunders DJ, Takei F. Dual Role of IL-7 in the Growth and Differentiation of Immature Thymocytes. Exp Hematol. 1992;20(8):998-1003. PubMed PMID: 1387092.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dual role of IL-7 in the growth and differentiation of immature thymocytes. AU - Vissinga,C S, AU - Fatur-Saunders,D J, AU - Takei,F, PY - 1992/9/1/pubmed PY - 1992/9/1/medline PY - 1992/9/1/entrez SP - 998 EP - 1003 JF - Experimental hematology JO - Exp Hematol VL - 20 IS - 8 N2 - The effects of interleukin 7 (IL-7) on subpopulations of CD4-CD8- thymocytes from young adult mice were tested in vitro. When highly purified CD3-CD4-CD8-thymocytes were cultured in the presence of recombinant IL-7, significant proportions of them became CD4+ and/or CD8+ within a day. CD3+ cells were also detected after 2 days. CD3-CD4-CD8- thymocytes were further subdivided into interleukin 2 receptor (IL-2R)- and IL-2R+ populations. The majority of the IL-2R- cells became CD4+ and/or CD8+ in 1 day in the presence of IL-7, and a substantial proportion of them also became CD3+ in 2-3 days. No significant number of CD4+ or CD8+ cells were generated from the IL-2R+ population under the same conditions. However, a small but significant proportion of them became CD3+ in 3-day cultures with IL-7. Although CD4+/CD8+ cells were also generated from the IL-2R- population in 1-day cultures in the absence of IL-7, the viability of the cells declined rapidly, and no significant numbers of CD3+ cells were generated. In proliferation assays, IL-7 alone vigorously stimulated relatively minor subpopulations of CD4-CD8- thymocytes. The IL-7-responsive cells were CD3+, did not express the IL-2R or the heat-stable antigen M1/69, and included both T-cell receptor (TCR)alpha beta + and TCR alpha beta- populations, the latter most likely TCR gamma delta +. The CD3+CD4-CD8- thymocytes, stimulated with IL-7 for 3 days, remained CD4-CD8-. These results demonstrate important roles of IL-7 in the growth and differentiation of CD4-CD8- thymocytes in vitro. It functions as a survival factor and allows CD3-CD4-CD8- cells to undergo their precommitted differentiation without inducing their proliferation, and it also stimulates CD3+CD4-CD8-thymocytes to proliferate without inducing their differentiation. SN - 0301-472X UR - https://www.unboundmedicine.com/medline/citation/1387092/Dual_role_of_IL_7_in_the_growth_and_differentiation_of_immature_thymocytes_ L2 - http://hivinsite.ucsf.edu/InSite?page=kb-02-01-04 DB - PRIME DP - Unbound Medicine ER -