Tags

Type your tag names separated by a space and hit enter

Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit.
Proc Natl Acad Sci U S A. 1992 Sep 15; 89(18):8769-73.PN

Abstract

U2 small nuclear ribonucleoprotein auxiliary factor (U2AF), an essential mammalian splicing factor, is composed of two subunits: a 65-kDa protein (U2AF65), which binds the pre-mRNA polypyrimidine tract and is required for in vitro splicing, and an associated 35-kDa protein (U2AF35). Here we report the isolation of a cDNA encoding U2AF35. U2AF35 contains sequence motifs found in several mammalian pre-mRNA splicing factors. We show directly that U2AF65 and U2AF35 interact with each other and delineate the regions of both proteins that mediate this interaction. Using anti-peptide antibodies against U2AF35, we show that the protein has the intracellular distribution characteristic of U2AF65. Both U2AF65 and U2AF35 are concentrated in a small number of nuclear foci corresponding to coiled bodies, subnuclear organelles first identified by light microscopy in 1903.

Authors+Show Affiliations

Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester 01605.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1388271

Citation

Zhang, M, et al. "Cloning and Intracellular Localization of the U2 Small Nuclear Ribonucleoprotein Auxiliary Factor Small Subunit." Proceedings of the National Academy of Sciences of the United States of America, vol. 89, no. 18, 1992, pp. 8769-73.
Zhang M, Zamore PD, Carmo-Fonseca M, et al. Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit. Proc Natl Acad Sci U S A. 1992;89(18):8769-73.
Zhang, M., Zamore, P. D., Carmo-Fonseca, M., Lamond, A. I., & Green, M. R. (1992). Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit. Proceedings of the National Academy of Sciences of the United States of America, 89(18), 8769-73.
Zhang M, et al. Cloning and Intracellular Localization of the U2 Small Nuclear Ribonucleoprotein Auxiliary Factor Small Subunit. Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8769-73. PubMed PMID: 1388271.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit. AU - Zhang,M, AU - Zamore,P D, AU - Carmo-Fonseca,M, AU - Lamond,A I, AU - Green,M R, PY - 1992/9/15/pubmed PY - 1992/9/15/medline PY - 1992/9/15/entrez SP - 8769 EP - 73 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 89 IS - 18 N2 - U2 small nuclear ribonucleoprotein auxiliary factor (U2AF), an essential mammalian splicing factor, is composed of two subunits: a 65-kDa protein (U2AF65), which binds the pre-mRNA polypyrimidine tract and is required for in vitro splicing, and an associated 35-kDa protein (U2AF35). Here we report the isolation of a cDNA encoding U2AF35. U2AF35 contains sequence motifs found in several mammalian pre-mRNA splicing factors. We show directly that U2AF65 and U2AF35 interact with each other and delineate the regions of both proteins that mediate this interaction. Using anti-peptide antibodies against U2AF35, we show that the protein has the intracellular distribution characteristic of U2AF65. Both U2AF65 and U2AF35 are concentrated in a small number of nuclear foci corresponding to coiled bodies, subnuclear organelles first identified by light microscopy in 1903. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/1388271/Cloning_and_intracellular_localization_of_the_U2_small_nuclear_ribonucleoprotein_auxiliary_factor_small_subunit_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=1388271 DB - PRIME DP - Unbound Medicine ER -