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A colorimetric method for the determination of carboxyhaemoglobin over a wide range of concentrations.
J Clin Pathol. 1962 Jan; 15:82-4.JC

Abstract

A colorimetric technique for the determination of carboxyhaemoglobin in blood is described. Carbon monoxide released from blood in a standard Conway unit reacts with palladous chloride/arsenomolybdate solution to produce a blue colour. Using 0.5 to 2 ml. of blood, the method will estimate carboxyhaemoglobin accurately at levels from 0.1% to 100% of total haemoglobin and in the presence of other abnormal pigments.A number of methods are available for the determination of carboxyhaemoglobin; none is accurate below a concentration of 1.5 g. carboxyhaemoglobin per 100 ml. but for most clinical purposes this is not important. For forensic purposes and occasionally in clinical use, an accurate determination of carboxyhaemoglobin below 750 mg. per 100 ml. may be required and no really satisfactory method is at present available. Some time ago when it was important to know whether a person who was found dead in a burning house had died before or after the fire had started, we became interested in developing a method which would determine accurately carboxyhaemoglobin at levels of 750 mg. per 100 ml.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

13922505

Citation

TRINDER, P, and F E. HARPER. "A Colorimetric Method for the Determination of Carboxyhaemoglobin Over a Wide Range of Concentrations." Journal of Clinical Pathology, vol. 15, 1962, pp. 82-4.
TRINDER P, HARPER FE. A colorimetric method for the determination of carboxyhaemoglobin over a wide range of concentrations. J Clin Pathol. 1962;15:82-4.
TRINDER, P., & HARPER, F. E. (1962). A colorimetric method for the determination of carboxyhaemoglobin over a wide range of concentrations. Journal of Clinical Pathology, 15, 82-4.
TRINDER P, HARPER FE. A Colorimetric Method for the Determination of Carboxyhaemoglobin Over a Wide Range of Concentrations. J Clin Pathol. 1962;15:82-4. PubMed PMID: 13922505.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A colorimetric method for the determination of carboxyhaemoglobin over a wide range of concentrations. AU - TRINDER,P, AU - HARPER,F E, PY - 1962/1/1/pubmed PY - 1962/1/1/medline PY - 1962/1/1/entrez KW - HEMOGLOBIN/chemistry SP - 82 EP - 4 JF - Journal of clinical pathology JO - J Clin Pathol VL - 15 N2 - A colorimetric technique for the determination of carboxyhaemoglobin in blood is described. Carbon monoxide released from blood in a standard Conway unit reacts with palladous chloride/arsenomolybdate solution to produce a blue colour. Using 0.5 to 2 ml. of blood, the method will estimate carboxyhaemoglobin accurately at levels from 0.1% to 100% of total haemoglobin and in the presence of other abnormal pigments.A number of methods are available for the determination of carboxyhaemoglobin; none is accurate below a concentration of 1.5 g. carboxyhaemoglobin per 100 ml. but for most clinical purposes this is not important. For forensic purposes and occasionally in clinical use, an accurate determination of carboxyhaemoglobin below 750 mg. per 100 ml. may be required and no really satisfactory method is at present available. Some time ago when it was important to know whether a person who was found dead in a burning house had died before or after the fire had started, we became interested in developing a method which would determine accurately carboxyhaemoglobin at levels of 750 mg. per 100 ml. SN - 0021-9746 UR - https://www.unboundmedicine.com/medline/citation/13922505/A_colorimetric_method_for_the_determination_of_carboxyhaemoglobin_over_a_wide_range_of_concentrations_ DB - PRIME DP - Unbound Medicine ER -
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