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Two anthranilate synthase genes in Arabidopsis: defense-related regulation of the tryptophan pathway.
Plant Cell. 1992 Jun; 4(6):721-33.PC

Abstract

Arabidopsis thaliana has two genes, ASA1 and ASA2, encoding the alpha subunit of anthranilate synthase, the enzyme catalyzing the first reaction in the tryptophan biosynthetic pathway. As a branchpoint enzyme in aromatic amino acid biosynthesis, anthranilate synthase has an important regulatory role. The sequences of the plant genes are homologous to their microbial counterparts. Both predicted proteins have putative chloroplast transit peptides at their amino termini and conserved amino acids involved in feedback inhibition by tryptophan. ASA1 and ASA2 cDNAs complement anthranilate synthase alpha subunit mutations in the yeast Saccharomyces cerevisiae and in Escherichia coli, confirming that both genes encode functional anthranilate synthase proteins. The distributions of ASA1 and ASA2 mRNAs in various parts of Arabidopsis plants are overlapping but nonidentical, and ASA1 mRNA is approximately 10 times more abundant in whole plants. Whereas ASA2 is expressed at a constitutive basal level, ASA1 is induced by wounding and bacterial pathogen infiltration, suggesting a novel role for ASA1 in the production of tryptophan pathway metabolites as part of an Arabidopsis defense response. Regulation of key steps in aromatic amino acid biosynthesis in Arabidopsis appears to involve differential expression of duplicated genes.

Authors+Show Affiliations

Department of Biology, Massachusetts Institute of Technology, Cambridge 02142.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

1392592

Citation

Niyogi, K K., and G R. Fink. "Two Anthranilate Synthase Genes in Arabidopsis: Defense-related Regulation of the Tryptophan Pathway." The Plant Cell, vol. 4, no. 6, 1992, pp. 721-33.
Niyogi KK, Fink GR. Two anthranilate synthase genes in Arabidopsis: defense-related regulation of the tryptophan pathway. Plant Cell. 1992;4(6):721-33.
Niyogi, K. K., & Fink, G. R. (1992). Two anthranilate synthase genes in Arabidopsis: defense-related regulation of the tryptophan pathway. The Plant Cell, 4(6), 721-33.
Niyogi KK, Fink GR. Two Anthranilate Synthase Genes in Arabidopsis: Defense-related Regulation of the Tryptophan Pathway. Plant Cell. 1992;4(6):721-33. PubMed PMID: 1392592.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Two anthranilate synthase genes in Arabidopsis: defense-related regulation of the tryptophan pathway. AU - Niyogi,K K, AU - Fink,G R, PY - 1992/6/1/pubmed PY - 1992/6/1/medline PY - 1992/6/1/entrez SP - 721 EP - 33 JF - The Plant cell JO - Plant Cell VL - 4 IS - 6 N2 - Arabidopsis thaliana has two genes, ASA1 and ASA2, encoding the alpha subunit of anthranilate synthase, the enzyme catalyzing the first reaction in the tryptophan biosynthetic pathway. As a branchpoint enzyme in aromatic amino acid biosynthesis, anthranilate synthase has an important regulatory role. The sequences of the plant genes are homologous to their microbial counterparts. Both predicted proteins have putative chloroplast transit peptides at their amino termini and conserved amino acids involved in feedback inhibition by tryptophan. ASA1 and ASA2 cDNAs complement anthranilate synthase alpha subunit mutations in the yeast Saccharomyces cerevisiae and in Escherichia coli, confirming that both genes encode functional anthranilate synthase proteins. The distributions of ASA1 and ASA2 mRNAs in various parts of Arabidopsis plants are overlapping but nonidentical, and ASA1 mRNA is approximately 10 times more abundant in whole plants. Whereas ASA2 is expressed at a constitutive basal level, ASA1 is induced by wounding and bacterial pathogen infiltration, suggesting a novel role for ASA1 in the production of tryptophan pathway metabolites as part of an Arabidopsis defense response. Regulation of key steps in aromatic amino acid biosynthesis in Arabidopsis appears to involve differential expression of duplicated genes. SN - 1040-4651 UR - https://www.unboundmedicine.com/medline/citation/1392592/Two_anthranilate_synthase_genes_in_Arabidopsis:_defense_related_regulation_of_the_tryptophan_pathway_ DB - PRIME DP - Unbound Medicine ER -